LOCUS       V046898                 9496 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046898
VERSION     V046898
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             925..5247
                     /label="NCOA1(NM_003743)"
                     /note="NCOA1(NM_003743)"
                     /gene="NCOA1"
     CDS             5260..5325
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     polyA_signal    5360..5584
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      5630..6058
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        6072..6401
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6413..7012
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     polyA_signal    7172..7293
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(7342..7358)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7366..7382
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7390..7420)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7435..7456
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7744..8329)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8500..9360)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9361..9465)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 aagctttcta gagaattcgc caccatgagt ggcctcgggg acagttcatc cgaccctgct
      961 aacccagact cacataagag gaaaggatcg ccatgtgaca cactggcatc aagcacggaa
     1021 aagaggcgca gggagcaaga aaataaatat ttagaagaac tagctgagtt actgtctgcc
     1081 aacattagtg acattgacag cttgagtgta aaaccagaca aatgcaagat tttgaagaaa
     1141 acagtcgatc agatacagct aatgaagaga atggaacaag agaaatcaac aactgatgac
     1201 gatgtacaga aatcagacat ctcatcaagt agtcaaggag tgatagaaaa ggaatccttg
     1261 ggacctcttc ttttggaggc tttggatgga tttttctttg ttgtgaactg tgaagggaga
     1321 attgtatttg tgtcagagaa tgtaaccagc tacttaggtt acaatcagga ggaattaatg
     1381 aatacgagcg tctacagcat actgcacgtg ggggatcatg cagaatttgt gaagaatctg
     1441 ctaccaaaat cactagtaaa tggagttcct tggcctcaag aggcaacacg acgaaatagc
     1501 cataccttta actgcaggat gctaattcac cctccagatg agccagggac cgagaaccaa
     1561 gaagcttgcc agcgttatga agtaatgcag tgtttcactg tgtcacagcc aaaatcaatt
     1621 caagaggatg gagaagattt ccagtcatgt ctgatttgta ttgcacggcg attacctcgg
     1681 cctccagcta ttacgggtgt agaatccttt atgaccaagc aagatactac aggtaaaatc
     1741 atctctattg atactagttc cctgagagct gctggcagaa ctggttggga agatttagtg
     1801 aggaagtgca tttatgcttt tttccaacct cagggcagag aaccatctta tgccagacag
     1861 ctgttccaag aagtgatgac tcgtggcact gcctccagcc cctcctatag attcatattg
     1921 aatgatggga caatgcttag cgcccacacc aagtgtaaac tttgctaccc tcaaagtcca
     1981 gacatgcaac ctttcatcat gggaattcat atcatcgaca gggagcacag tgggctttct
     2041 cctcaagatg acactaattc tggaatgtca attccccgag taaatccctc ggtcaatcct
     2101 agtatctctc cagctcatgg tgtggctcgt tcatccacat tgccaccatc caacagcaac
     2161 atggtatcca ccagaataaa ccgccagcag agctcagacc ttcatagcag cagtcatagt
     2221 aattctagca acagccaagg aagtttcgga tgctcacccg gaagtcagat tgtagccaat
     2281 gttgccttaa accaaggaca ggccagttca cagagcagta atccctcttt aaacctcaat
     2341 aattctccta tggaaggtac aggaatatcc ctagcacagt tcatgtctcc aaggagacag
     2401 gttacttctg gattggcaac aaggcccagg atgccaaaca attcctttcc tcctaatatt
     2461 tcgacattaa gctctcccgt tggcatgaca agtagtgcct gtaataataa taaccgatct
     2521 tattcaaaca tcccagtaac atctttacag ggtatgaatg aaggacccaa taactccgtt
     2581 ggcttctctg ccagttctcc agtcctcagg cagatgagct cacagaattc acctagcaga
     2641 ttaaatatac aaccagcaaa agctgagtcc aaagataaca aagagattgc ctcaatttta
     2701 aatgaaatga ttcaatctga caacagctct agtgatggca aacctctgga ttcagggctt
     2761 ctgcataaca atgacagact ttcagatgga gacagtaaat actctcaaac cagtcacaaa
     2821 ctagtgcagc ttttgacaac aactgccgaa cagcagttac ggcatgctga tatagacaca
     2881 agctgcaaag atgtcctgtc ttgcacaggc acttccaact ctgcctctgc taactcttca
     2941 ggaggttctt gtccctcttc tcatagctca ttgacagaac ggcataaaat tctacaccgg
     3001 ctcttacagg agggtagccc ctcagatatc accactttgt ctgtcgagcc tgataaaaag
     3061 gacagtgcat ctacttctgt gtcagtgact ggacaggtac aaggaaactc cagtataaaa
     3121 ctagaactgg atgcttcaaa gaaaaaagaa tcaaaagacc atcagctcct acgctatctt
     3181 ttagataaag atgagaaaga tttaagatca actccaaacc tgagcctgga tgatgtaaag
     3241 gtgaaagtgg aaaagaaaga acagatggat ccatgtaata caaacccaac cccaatgacc
     3301 aaacccactc ctgaggaaat aaaactggag gcccagagcc agtttacagc tgaccttgac
     3361 cagtttgatc agttactgcc cacgctggag aaggcagcac agttgccagg cttatgtgag
     3421 acagacagga tggatggtgc ggtcaccagt gtaaccatca aatcggagat cctgccagct
     3481 tcacttcagt ccgccactgc cagacccact tccaggctaa atagattacc tgagctggaa
     3541 ttggaagcaa ttgataacca atttggacaa ccaggaacag gcgatcagat tccatggaca
     3601 aataatacag tgacagctat aaatcagagt aaatcagaag accagtgtat tagctcacaa
     3661 ttagatgagc ttctctgtcc acccacaaca gtagaaggga gaaatgatga gaaggctctt
     3721 cttgaacagc tggtatcctt ccttagtggc aaagatgaaa ctgagctagc tgaactagac
     3781 agagctctgg gaattgacaa acttgttcag gggggtggat tagatgtatt atcagagaga
     3841 tttccaccac aacaagcaac gccacctttg atcatggaag aaagacccaa cctttattcc
     3901 cagccttact cttctccttc tcctactgcc aatctcccta gccctttcca aggcatggtc
     3961 aggcaaaaac cttcactggg gacgatgcct gttcaagtaa cacctccccg aggtgctttt
     4021 tcacctggca tgggcatgca gcccaggcaa actctaaaca gacctccggc tgcacctaac
     4081 cagcttcgac ttcaactaca gcagcgatta cagggacaac agcagttgat acaccaaaat
     4141 cggcaagcta tcttaaacca gtttgcagca actgctcctg ttggcatcaa tatgagatca
     4201 ggcatgcaac agcaaattac acctcagcca cccctgaatg ctcaaatgtt ggcacaacgt
     4261 cagcgggaac tgtacagtca acagcaccga cagaggcagc taatacagca gcaaagagcc
     4321 atgcttatga ggcagcaaag ctttgggaac aacctccctc cctcatctgg actaccagtt
     4381 caaatgggga acccccgtct tcctcagggt gctccacagc aattccccta tccaccaaac
     4441 tatggtacaa atccaggaac cccacctgct tctaccagcc cgttttcaca actagcagca
     4501 aatcctgaag catccttggc caaccgcaac agcatggtga gcagaggcat gacaggaaac
     4561 ataggaggac agtttggcac tggaatcaat cctcagatgc agcagaatgt cttccagtat
     4621 ccaggagcag gaatggttcc ccaaggtgag gccaactttg ctccatctct aagccctggg
     4681 agctccatgg tgccgatgcc aatccctcct cctcagagtt ctcttctcca gcaaactcca
     4741 cctgcctccg ggtatcagtc accagacatg aaggcctggc agcaaggagc gataggaaac
     4801 aacaatgtgt tcagtcaagc tgtccagaac cagcccacgc ctgcacagcc aggagtatac
     4861 aacaacatga gcatcaccgt ttccatggca ggtggaaata cgaatgttca gaacatgaac
     4921 ccaatgatgg cccagatgca gatgagctct ttgcagatgc caggaatgaa cactgtgtgc
     4981 cctgagcaga taaatgatcc cgcactgaga cacacaggcc tctactgcaa ccagctctca
     5041 tccactgacc ttctcaaaac agaagcagat ggaacccagc aggtgcaaca ggttcaggtg
     5101 tttgctgacg tccagtgtac agtgaatctg gtaggcgggg acccttacct gaaccagcct
     5161 ggtccactgg gaactcaaaa gcccacgtca ggaccacaga ccccccaggc ccagcagaag
     5221 agcctccttc agcagctact gactgaactc gagaccggtg actacaaaga ccatgacggt
     5281 gattataaag atcatgacat cgattacaag gatgacgatg acaagtgacc ggtaagttta
     5341 aaccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     5401 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     5461 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     5521 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     5581 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     5641 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     5701 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     5761 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     5821 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     5881 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     5941 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     6001 gccatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     6061 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     6121 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     6181 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     6241 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     6301 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     6361 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggatgaccga
     6421 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     6481 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     6541 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     6601 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     6661 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     6721 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     6781 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     6841 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     6901 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     6961 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gattcgaaat
     7021 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
     7081 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     7141 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     7201 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     7261 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     7321 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     7381 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     7441 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     7501 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     7561 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     7621 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     7681 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     7741 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     7801 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     7861 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     7921 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     7981 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     8041 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     8101 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     8161 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     8221 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     8281 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     8341 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     8401 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     8461 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     8521 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     8581 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     8641 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     8701 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     8761 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     8821 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     8881 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     8941 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     9001 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     9061 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     9121 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     9181 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     9241 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     9301 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     9361 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     9421 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     9481 agtgccacct gacgtc
//
