LOCUS       40924_12005        6507 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for constitutive expression of VSV-G glycoprotein. Also known
            as pHCMV-G or pHCMV-VSV-G.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6507)
  AUTHORS   Yee JK, Miyanohara A, LaPorte P, Bouic K, Burns JC, Friedmann T.
  TITLE     A general method for the generation of high-titer, pantropic 
            retroviral vectors: highly efficient infection of primary 
            hepatocytes.
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 1994;91:9564-8.
  PUBMED    7937806
REFERENCE   2  (bases 1 to 6507)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6507)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "1994"; volume: "91"; pages: "9564-8"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     The sequence from GenBank accession number AJ318514 was edited to 
            match data associated with Addgene plasmid number 8454.
FEATURES             Location/Qualifiers
     source          1..6507
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        83..462
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        463..666
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          778..1350
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             1436..2968
                     /codon_start=1
                     /label=VSV-G
                     /note="vesicular stomatitis virus G glycoprotein"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     polyA_signal    3263..3318
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3676..3692)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3700..3716)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3724..3754)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3769..3790)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4078..4666)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4840..5697)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5698..5802)
                     /label=AmpR promoter
     rep_origin      complement(5828..6283)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     6425..6441
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6451..6469
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gagcttggcc cattgcatac gttgtatcca tatcataata tgtacattta tattggctca
       61 tgtccaacat taccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt
      121 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
      181 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
      241 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
      301 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
      361 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
      421 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
      481 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
      541 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
      601 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
      661 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
      721 catagaagac accgggaccg atccagcctc cggtcgaccg atcctgagaa cttcagggtg
      781 agtttgggga cccttgattg ttctttcttt ttcgctattg taaaattcat gttatatgga
      841 gggggcaaag ttttcagggt gttgtttaga atgggaagat gtcccttgta tcaccatgga
      901 ccctcatgat aattttgttt ctttcacttt ctactctgtt gacaaccatt gtctcctctt
      961 attttctttt cattttctgt aactttttcg ttaaacttta gcttgcattt gtaacgaatt
     1021 tttaaattca cttttgttta tttgtcagat tgtaagtact ttctctaatc actttttttt
     1081 caaggcaatc agggtatatt atattgtact tcagcacagt tttagagaac aattgttata
     1141 attaaatgat aaggtagaat atttctgcat ataaattctg gctggcgtgg aaatattctt
     1201 attggtagaa acaactacac cctggtcatc atcctgcctt tctctttatg gttacaatga
     1261 tatacactgt ttgagatgag gataaaatac tctgagtcca aaccgggccc ctctgctaac
     1321 catgttcatg ccttcttctc tttcctacag ctcctgggca acgtgctggt tgttgtgctg
     1381 tctcatcatt ttggcaaaga attcctcgac ggatccctcg aggaattctg acactatgaa
     1441 gtgccttttg tacttagcct ttttattcat tggggtgaat tgcaagttca ccatagtttt
     1501 tccacacaac caaaaaggaa actggaaaaa tgttccttct aattaccatt attgcccgtc
     1561 aagctcagat ttaaattggc ataatgactt aataggcaca gccttacaag tcaaaatgcc
     1621 caagagtcac aaggctattc aagcagacgg ttggatgtgt catgcttcca aatgggtcac
     1681 tacttgtgat ttccgctggt atggaccgaa gtatataaca cattccatcc gatccttcac
     1741 tccatctgta gaacaatgca aggaaagcat tgaacaaacg aaacaaggaa cttggctgaa
     1801 tccaggcttc cctcctcaaa gttgtggata tgcaactgtg acggatgccg aagcagtgat
     1861 tgtccaggtg actcctcacc atgtgctggt tgatgaatac acaggagaat gggttgattc
     1921 acagttcatc aacggaaaat gcagcaatta catatgcccc actgtccata actctacaac
     1981 ctggcattct gactataagg tcaaagggct atgtgattct aacctcattt ccatggacat
     2041 caccttcttc tcagaggacg gagagctatc atccctggga aaggagggca cagggttcag
     2101 aagtaactac tttgcttatg aaactggagg caaggcctgc aaaatgcaat actgcaagca
     2161 ttggggagtc agactcccat caggtgtctg gttcgagatg gctgataagg atctctttgc
     2221 tgcagccaga ttccctgaat gcccagaagg gtcaagtatc tctgctccat ctcagacctc
     2281 agtggatgta agtctaattc aggacgttga gaggatcttg gattattccc tctgccaaga
     2341 aacctggagc aaaatcagag cgggtcttcc aatctctcca gtggatctca gctatcttgc
     2401 tcctaaaaac ccaggaaccg gtcctgcttt caccataatc aatggtaccc taaaatactt
     2461 tgagaccaga tacatcagag tcgatattgc tgctccaatc ctctcaagaa tggtcggaat
     2521 gatcagtgga actaccacag aaagggaact gtgggatgac tgggcaccat atgaagacgt
     2581 ggaaattgga cccaatggag ttctgaggac cagttcagga tataagtttc ctttatacat
     2641 gattggacat ggtatgttgg actccgatct tcatcttagc tcaaaggctc aggtgttcga
     2701 acatcctcac attcaagacg ctgcttcgca acttcctgat gatgagagtt tattttttgg
     2761 tgatactggg ctatccaaaa atccaatcga gcttgtagaa ggttggttca gtagttggaa
     2821 aagctctatt gcctcttttt tctttatcat agggttaatc attggactat tcttggttct
     2881 ccgagttggt atccatcttt gcattaaatt aaagcacacc aagaaaagac agatttatac
     2941 agacatagag atgaaccgac ttggaaagta actcaaatcc tgcacaacag attcttcatg
     3001 tttggaccaa atcaacttgt gataccatgc tcaaagaggc ctcaattata tttgagtttt
     3061 taatttttat gaaaaaaaaa aaaaaaaacg gaattcctcg agggatccgt cgaggaattc
     3121 actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa tgccctggct
     3181 cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat catgaagccc
     3241 cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg
     3301 aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag
     3361 aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa
     3421 ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc
     3481 catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt
     3541 tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc
     3601 tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga cggatcggcc
     3661 gcaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     3721 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     3781 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     3841 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     3901 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     3961 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     4021 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     4081 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     4141 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     4201 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     4261 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     4321 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     4381 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     4441 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     4501 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     4561 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     4621 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     4681 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     4741 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     4801 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     4861 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     4921 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     4981 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     5041 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     5101 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     5161 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     5221 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     5281 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     5341 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     5401 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     5461 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     5521 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     5581 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     5641 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     5701 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     5761 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     5821 gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     5881 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     5941 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     6001 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     6061 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     6121 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     6181 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     6241 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg
     6301 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     6361 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     6421 cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg cgaattggag
     6481 ctccaccgcg gtggcggccg ctctaga
//
