LOCUS       V034857                 4668 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034857
VERSION     V034857
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        complement(join(4636..4668,1..72))
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     546..562
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        589..892
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        893..1096
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     intron          1234..1330
                     /note="modified SV40 intron with splice donor and acceptor
                     sites"
                     /label="SV40 intron"
     CDS             1394..1420
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             1448..2344
                     /label="ZDHHC3(NM_001135179)"
                     /note="ZDHHC3(NM_001135179)"
                     /gene="ZDHHC3"
     polyA_signal    2370..2504
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(2614..2630)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    2638..2654
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(2662..2692)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    2707..2728
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(3016..3604)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(3775..4635)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
ORIGIN
        1 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
       61 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
      121 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
      181 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
      241 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
      301 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
      361 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag
      421 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
      481 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
      541 acgttgtaaa acgacggcca gtgagttcga gcttgcatgc ctgcaggtcg ttacataact
      601 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
      661 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
      721 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
      781 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
      841 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
      901 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
      961 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     1021 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     1081 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
     1141 ttttgacctc catagaagac accgggaccg atccagcctc cggactctag aggatccggt
     1201 actagaggaa ctgaaaaacc agaaagttaa ctggtaagtt tagtcttttt gtcttttatt
     1261 tcaggtcccg gatccggtgg tggtgcaaat caaagaactg ctcctcagtg gatgttgcct
     1321 ttacttctag gcctgtacgg aagtgttact tctgctctaa aagctgcgga attgtacccg
     1381 cgggcccacc atgtacccat acgatgttcc agattacgct cttatggcca tggaggcccg
     1441 aattcccatg atgcttatcc ccacccacca cttccgaaac attgagcgga aaccagaata
     1501 cctccagcca gagaagtgtg tcccaccccc ctaccctggt cctgtgggaa ccatgtggtt
     1561 tatccgtgac ggctgtggca tcgcctgtgc catcgttacc tggtttctgg tcctctatgc
     1621 ggagttcgtg gtcctctttg tcatgctgat tccatctcga gactacgtgt atagcatcat
     1681 caacggaatt gtgttcaacc tgctggcctt cttggccctg gcctcccact gccgggccat
     1741 gctgacggac cccggggcag tgcccaaagg aaatgccact aaagaattca tcgagagttt
     1801 acagttgaag cctgggcagg tggtgtacaa gtgccccaaa tgctgcagca tcaagcccga
     1861 ccgagcccac cactgcagtg tttgtaagcg gtgcattcgg aagatggacc accactgtcc
     1921 ctgggtcaac aactgtgtag gcgagaacaa ccagaagtac ttcgtcctgt ttacaatgta
     1981 catagctctc atttccttgc acgccctcat catggtggga ttccacttcc tgcattgctt
     2041 tgaagaagat tggacaaagt gcagctcctt ctctccaccc accacagtga ttctccttat
     2101 cctgctgtgc tttgagggcc tgctcttcct cattttcaca tcagtgatgt ttgggaccca
     2161 ggtgcactcc atctgcacag atgagacggg aatagaacaa ttgaaaaagg aagagagaag
     2221 atgggctaaa aaaacaaaat ggatgaacat gaaagccgtt tttggccacc ccttctctct
     2281 aggctgggcc agcccctttg ccacgccaga ccaagggaag gcagacccgt accagtatgt
     2341 ggtctgactc gaggtaccgc ggccgcgggg atccagacat gataagatac attgatgagt
     2401 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     2461 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
     2521 ttcattttat gtttcaggtt cagggggagg tgtgggaggt ttttcggatc ctctagagtc
     2581 gatctgcagg catgctagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     2641 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     2701 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     2761 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     2821 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     2881 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     2941 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     3001 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     3061 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     3121 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     3181 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     3241 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     3301 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     3361 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     3421 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     3481 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     3541 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     3601 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     3661 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     3721 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     3781 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     3841 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     3901 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     3961 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     4021 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     4081 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     4141 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     4201 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     4261 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     4321 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     4381 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     4441 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     4501 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     4561 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     4621 atgttgaata ctcatactct tcctttttca atattattga agcattta
//
