LOCUS       pCMV6-AC-3HA.        5989 bp DNA     circular SYN 01-JAN-1980
DEFINITION  PrecisionShuttle(TM) mammalian expression vector for fusing an ORF 
            to a C-terminal 3xHA tag.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV6-AC-3HA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5989)
  AUTHORS   OriGene
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5989)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Inserts are typically cloned between the SgfI (AsiSI) and MluI 
            sites.
FEATURES             Location/Qualifiers
     source          1..5989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     166..182
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        343..722
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        723..926
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        952..970
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    979..1087
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             1088..1177
                     /label=3xHA
                     /note="three tandem HA epitope tags"
     polyA_signal    1224..1846
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     promoter        1875..2204
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2271..3062
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    3239..3372
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3409..3425)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3433..3449)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3457..3487)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3502..3523)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3811..4396)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4570..5427)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5428..5532)
                     /label=AmpR promoter
     rep_origin      5559..5989
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccgg cgcgccagat ctcaagctta actagctagc ggaccgacgc gtacgcggcc
     1081 gctcgagtac ccatacgatg ttccagatta cgctggctat ccctatgacg tcccggacta
     1141 tgcaggatcc tatccatatg acgttccaga ttacgctgtt taaacggccg gccgcggtca
     1201 tagctgtttc ctgaacagat cccgggtggc atccctgtga cccctcccca gtgcctctcc
     1261 tggccctgga agttgccact ccagtgccca ccagccttgt cctaataaaa ttaagttgca
     1321 tcattttgtc tgactaggtg tccttctata atattatggg gtggaggggg gtggtatgga
     1381 gcaaggggca agttgggaag acaacctgta gggcctgcgg ggtctattgg gaaccaagct
     1441 ggagtgcagt ggcacaatct tggctcactg caatctccgc ctcctgggtt caagcgattc
     1501 tcctgcctca gcctcccgag ttgttgggat tccaggcatg catgaccagg ctcagctaat
     1561 ttttgttttt ttggtagaga cggggtttca ccatattggc caggctggtc tccaactcct
     1621 aatctcaggt gatctaccca ccttggcctc ccaaattgct gggattacag gcgtgaacca
     1681 ctgctccctt ccctgtcctt ctgattttaa aataactata ccagcaggag gacgtccaga
     1741 cacagcatag gctacctggc catgcccaac cggtgggaca tttgagttgc ttgcttggca
     1801 ctgtcctctc atgcgttggg tccactcagt agatgcctgt tgaattgggt acgcggccag
     1861 cttggctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     1921 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     1981 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     2041 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     2101 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     2161 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     2221 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
     2281 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     2341 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     2401 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     2461 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     2521 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     2581 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     2641 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     2701 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     2761 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     2821 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     2881 ctggattcat cgactgtggc cggctgggtg tggccgaccg ctatcaggac atagcgttgg
     2941 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     3001 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     3061 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     3121 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     3181 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     3241 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     3301 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     3361 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     3421 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     3481 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     3541 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     3601 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     3661 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     3721 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     3781 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     3841 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     3901 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     3961 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     4021 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     4081 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     4141 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     4201 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     4261 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     4321 cggcaaacaa accaccgctg gtagcggttt ttttgtttgc aagcagcaga ttacgcgcag
     4381 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     4441 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     4501 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     4561 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     4621 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     4681 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     4741 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     4801 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     4861 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     4921 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     4981 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     5041 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     5101 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     5161 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     5221 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     5281 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     5341 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     5401 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     5461 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     5521 aggggttccg cgcacatttc cccgaaaagt gccacctgac gcgccctgta gcggcgcatt
     5581 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     5641 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     5701 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     5761 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     5821 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     5881 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     5941 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttt
//
