LOCUS       Exported                5438 bp DNA     circular SYN 31-AUG-2024
DEFINITION  Exported.
ACCESSION   V001682
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5438)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5438)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5438
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          644..649
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          1126..1131
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             21..407
                     /gene="cheY"
                     /label=Chemotaxis protein CheY
                     /note="Chemotaxis protein CheY from Escherichia coli
                     (strain K12). Accession#: P0AE67"
     primer_bind     complement(465..481)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      668..1123
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1156..1260
                     /label=AmpR promoter
     CDS             1261..2118
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2292..2880
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(3066..3207)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(3616..3789)
                     /label=lacZ-alpha
                     /note="LacZ-alpha fragment of beta-galactosidase"
     protein_bind    complement(3809..3825)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3833..3863)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3878..3899)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(3915..4994)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(4995..5072)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     protein_bind    5124..5145
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5160..5190
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    5198..5214
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        5269..5297
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    5305..5321
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    5364..5392
                     /label=tac promoter
                     /note="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    5400..5416
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 atcgatgctt aggaggtcat atggcggata aagaacttaa atttttggtt gtggatgact
       61 tttccaccat gcgacgcata gtgcgtaacc tgctgaaaga gctgggattc aataatgttg
      121 aggaagcgga agatggcgtc gacgctctca ataagttgca ggcaggcggt tatggatttg
      181 ttatctccga ctggaacatg cccaatatgg atggcctgga attgctgaaa acaattcgtg
      241 cggatggcgc gatgtcggca ttgccagtgt taatggtgac tgcagaagcg aagaaagaga
      301 acatcattgc tgcggcgcaa gcgggggcca gtggctatgt ggtgaagcca tttaccgccg
      361 cgacgctgga ggaaaaactc aacaaaatct ttgagaaact gggcatgtga ggatgcgact
      421 atgatgcaac catcaatcaa acctgctgac gagcattcag gggggatcca ctagttctag
      481 agtcgacctg cagcccaagc ttatcgatga taagctgtca aacatgagca gatctgagcc
      541 cgcctaatga gcgggctttt ttttcagatc tgcttgaaga cgaaagggcc tcgtgatacg
      601 cctattttta taggttaatg tcatgataat aatggtttct tagacgatgc gtcaaagcaa
      661 ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
      721 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
      781 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
      841 cgatttagag ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt
      901 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
      961 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggg ctattctttt
     1021 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     1081 aaatttaacg cgaattttaa caaaatatta acgtttacaa tttcatcgtc aggtggcact
     1141 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     1201 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     1261 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     1321 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     1381 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     1441 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     1501 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     1561 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     1621 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     1681 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     1741 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     1801 cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     1861 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     1921 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     1981 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     2041 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     2101 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     2161 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     2221 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     2281 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     2341 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     2401 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     2461 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     2521 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     2581 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     2641 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     2701 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     2761 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     2821 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     2881 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     2941 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     3001 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     3061 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcataatg
     3121 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta
     3181 tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc
     3241 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     3301 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagaa cgccatcaaa
     3361 aataattcgc gtctggcctt cctgtagcca gctttcatca acattaaatg tgagcgagta
     3421 acaacccgtc ggattctccg tgggaacaaa cggcggattg accgtaatgg gataggtcac
     3481 gttggtgtag atgggcgcat cgtaaccgtg catctgccag tttgagggga cgacgacagt
     3541 atcggcctca ggaagatcgc actccagcca gctttccggc accgcttctg gtgccggaaa
     3601 ccaggcaaag cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     3661 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     3721 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aatccgtaat
     3781 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacattatac
     3841 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa cttacattaa
     3901 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     3961 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt ggtttttctt
     4021 ttcaccagtg agacgggcaa cagctgattg cccttcaccg cctggccctg agagagttgc
     4081 agcaagcggt ccacgctggt ttgccccagc aggcgaaaat cctgtttgat ggtggttaac
     4141 ggcgggatat aacatgagct gtcttcggta tcgtcgtatc ccactaccga gatatccgca
     4201 ccaacgcgca gcccggactc ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg
     4261 gcaaccagca tcgcagtggg aacgatgccc tcattcagca tttgcatggt ttgttgaaaa
     4321 ccggacatgg cactccagtc gccttcccgt tccgctatcg gctgaatttg attgcgagtg
     4381 agatatttat gccagccagc cagacgcaga cgcgccgaga cagaacttaa tgggcccgct
     4441 aacagcgcga tttgctggtg acccaatgcg accagatgct ccacgcccag tcgcgtaccg
     4501 tcttcatggg agaaaataat actgttgatg ggtgtctggt cagagacatc aagaaataac
     4561 gccggaacat tagtgcaggc agcttccaca gcaatggcat cctggtcatc cagcggatag
     4621 ttaatgatca gcccactgac gcgttgcgcg agaagattgt gcaccgccgc tttacaggct
     4681 tcgacgccgc ttcgttctac catcgacacc accacgctgg cacccagttg atcggcgcga
     4741 gatttaatcg ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg
     4801 ccaatcagca acgactgttt gcccgccagt tgttgtgcca cgcggttggg aatgtaattc
     4861 agctccgcca tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg gctggcctgg
     4921 ttcaccacgc gggaaacggt ctgataagag acaccggcat actctgcgac atcgtataac
     4981 gttactggtt tcacattcac caccctgaat tgactctctt ccgggcgcta tcatgccata
     5041 ccgcgaaagg ttttgcacca ttcgatggtg tcctggcacg acaggtttcc cgactggaaa
     5101 gcgggcagtg agcgcaacgc aattaatgta agttagctca ctcattaggc accccaggct
     5161 ttacacttta tgcttccggc tcgtataatg tgtggaattg tgagcggata acaatttcac
     5221 acaggaaaca ggatcgatcc atcgatgagc ttactcccca tccccctgtt gacaattaat
     5281 catcggctcg tataatgtgt ggaattgtga gcggataaca atttcacaca ggaaacagga
     5341 tcagcttact ccccatcccc ctgttgacaa ttaatcatcg gctcgtataa tgtgtggaat
     5401 tgtgagcgga taacaatttc acacaggaaa caggatcc
//
