LOCUS       V008013                10407 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V008013
VERSION     V008013
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10407)
  AUTHORS   Krasny L, Gourse RL.
  TITLE     rRNA promoter strength and regulation in Bacillus subtilis: an
            alternative strategy for the modulation of ribosome synthesis in
            bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10407)
  AUTHORS   Krasny L, Gourse RL.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2004) Bacteriology, University of Wisconsin,
            Madison, 420 Henry Mall, Madison, WI 53706, USA
REFERENCE   3  (bases 1 to 10407)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10407)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-MAY-2004) Bacteriology, University of Wisconsin, Madison, 420
            Henry Mall, Madison, WI 53706, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10407
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            115..635
                     /gene="amyE"
                     /label="amyE"
                     /note="amyE front; partial alpha-amylase; similar to
                     amyE-front in GenBank Accession Number U46196"
     regulatory      751..837
                     /note="derived from Bacillus subtilis rrnB; terminates
                     transcription from amyE front"
                     /regulatory_class="terminator"
     regulatory      complement(844..932)
                     /note="derived from Bacillus subtilis rrnO; terminates lacZ
                     transcription"
                     /regulatory_class="terminator"
     CDS             complement(950..3982)
                     /label="lacZ"
                     /note="beta-galactosidase"
     CDS             4391..5038
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     gene            5412..6440
                     /gene="amyE"
                     /label="amyE"
                     /note="amyE back; partial alpha-amylase; similar to
                     amyE-back in GenBank Accession Number U46196"
     CDS             7549..8328
                     /gene="ant1"
                     /label="Spectinomycin 9-adenylyltransferase"
                     /note="Spectinomycin 9-adenylyltransferase from
                     Staphylococcus aureus (strain N315). Accession#: P0A0D1"
     rep_origin      complement(8578..9166)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9340..10197)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(10198..10302)
                     /label="AmpR promoter"
ORIGIN
        1 aacaaaattc tccagtcttc acatcggttt gaaaggagga agcggaagaa tgaagtaaga
       61 gggatttttg actccgaagt aagtcttcaa aaaatcaaat aaggagtgtc aagaatgttt
      121 gcaaaacgat tcaaaacctc tttactgccg ttattcgctg gatttttatt gctgtttcat
      181 ttggttctgg caggaccggc ggctgcgagt gctgaaacgg cgaacaaatc gaatgagctt
      241 acagcaccgt cgatcaaaag cggaaccatt cttcatgcat ggaattggtc gttcaatacg
      301 ttaaaacaca atatgaagga tattcatgat gcaggatata cagccattca gacatctccg
      361 attaaccaag taaaggaagg gaatcaagga gataaaagca tgtcgaactg gtactggctg
      421 tatcagccga catcgtatca aattggcaac cgttacttag gtactgaaca agaatttaaa
      481 gaaatgtgtg cagccgctga agaatatggc ataaaggtca ttgttgacgc ggtcatcaat
      541 cataccacca gtgattatgc cgcgatttcc aatgaggtta agagtattcc aaactggaca
      601 catggaaaca cacaaattaa aaactggtct gatcgatagt acataatgga tttccttacg
      661 cgaaatacgg gcagacatgg cctgcccggt tattattatt tttgacacca gaccaactgg
      721 taatggtagc gaccggcgct cacatccgga gttcgaatcc cgtacgggtc atccagaagc
      781 cttgcatatc ctgcaaggtt tttttgtttt tataaatcat gatatgtctt agattttact
      841 agttaaatac gcttcacagt ttcttcttca tttttaacaa acaaaaaacc cagctcattg
      901 agctgggttt aagctggctt ggcggcgtcc taggatccgg ttattattat ttttgacacc
      961 agaccaactg gtaatggtag cgaccggcgc tcagctgaaa ttccgccgat actgacgggc
     1021 tccaggagtc gtcgccacca atccccatat ggaaaccgtc gatattcagc catgtgcctt
     1081 cttccgcgtg cagcagatgg cgatggctgg tttccatcag ttgctgttga ctgtagcggc
     1141 tgatgttgaa ctggaagtcg ccgcgccact ggtgtgggcc ataattcaat tcgcgcgtcc
     1201 cgcagcgcag accgttttcg ctcgggaaga cgtacggggt atacatgtct gacaatggca
     1261 gatcccagcg gtcaaaacag gcggcagtaa ggcggtcggg atagttttct tgcggcccta
     1321 atccgagcca gtttacccgc tctgctacct gcgccagctg gcagttcagg ccaatccgcg
     1381 ccggatgcgg tgtatcgctc gccacttcaa catcaacggt aatcgccatt tgaccactac
     1441 catcaatccg gtaggttttc cggctgataa ataaggtttt cccctgatgc tgccacgcgt
     1501 gagcggtcgt aatcagcacc gcatcagcaa gtgtatctgc cgtgcactgc aacaacgctg
     1561 cttcggcctg gtaatggccc gccgccttcc agcgttcgac ccaggcgtta gggtcaatgc
     1621 gggtcgcttc acttacgcca atgtcgttat ccagcggtgc acgggtgaac tgatcgcgca
     1681 gcggcgtcag cagttgtttt ttatcgccaa tccacatctg tgaaagaaag cctgactggc
     1741 ggttaaattg ccaacgctta ttacccagct cgatgcaaaa atccatttcg ctggtggtca
     1801 gatgcgggat ggcgtgggac gcggcgggga gcgtcacact gaggttttcc gccagacgcc
     1861 actgctgcca ggcgctgatg tgcccggctt ctgaccatgc ggtcgcgttc ggttgcacta
     1921 cgcgtactgt gagccagagt tgcccggcgc tctccggctg cggtagttca ggcagttcaa
     1981 tcaactgttt accttgtgga gcgacatcca gaggcacttc accgcttgcc agcggcttac
     2041 catccagcgc caccatccag tgcaggagct cgttatcgct atgacggaac aggtattcgc
     2101 tggtcacttc gatggtttgc ccggataaac ggaactggaa aaactgctgc tggtgttttg
     2161 cttccgtcag cgctggatgc ggcgtgcggt cggcaaagac cagaccgttc atacagaact
     2221 ggcgatcgtt cggcgtatcg ccaaaatcac cgccgtaagc cgaccacggg ttgccgtttt
     2281 catcatattt aatcagcgac tgatccaccc agtcccagac gaagccgccc tgtaaacggg
     2341 gatactgacg aaacgcctgc cagtatttag cgaaaccgcc aagactgtta cccatcgcgt
     2401 gggcgtattc gcaaaggatc agcgggcgcg tctctccagg tagcgaaagc cattttttga
     2461 tggaccattt cggcacagcc gggaagggct ggtcttcatc cacgcgcgcg tacatcgggc
     2521 aaataatatc ggtggccgtg gtgtcggctc cgccgccttc atactgcacc gggcgggaag
     2581 gatcgacaga tttgatccag cgatacagcg cgtcgtgatt agcgccgtgg cctgattcat
     2641 tccccagcga ccagatgatc acactcgggt gattacgatc gcgctgcacc attcgcgtta
     2701 cgcgttcgct catcgccggt agccagcgcg gatcatcggt cagacgattc attggcacca
     2761 tgccgtgggt ttcaatattg gcttcatcca ccacatacag gccgtagcgg tcgcacagcg
     2821 tgtaccacag cggatggttc ggataatgcg aacagcgcac ggcgttaaag ttgttctgct
     2881 tcatcagcag gatatcctgc accatcgtct gctcatccat gacctgacca tgcagaggat
     2941 gatgctcgtg acggttaacg cctcgaatca gcaacggctt gccgttcagc agcagcagac
     3001 cattttcaat ccgcacctcg cggaaaccga catcgcaggc ttctgcttca atcagcgtgc
     3061 cgtcggcggt gtgcagttca accaccgcac gatagagatt cgggatttcg gcgctccaca
     3121 gtttcgggtt ttcgacgttc agacgtagtg tgacgcgatc ggcataacca ccacgctcat
     3181 cgataatttc accgccgaaa ggcgcggtgc cgctggcgac ctgcgtttca ccctgccata
     3241 aagaaactgt tacccgtagg tagtcacgca actcgccgca catctgaact tcagcctcca
     3301 gtacagcgcg gctgaaatca tcattaaagc gagtggcaac atggaaatcg ctgatttgtg
     3361 tagtcggttt atgcagcaac gagacgtcac ggaaaatgcc gctcatccgc cacatatcct
     3421 gatcttccag ataactgccg tcactccaac gcagcaccat caccgcgagg cggttttctc
     3481 cggcgcgtaa aaatgcgctc aggtcaaatt cagacggcaa acgactgtcc tggccgtaac
     3541 cgacccagcg cccgttgcac cacagatgaa acgccgagtt aacgccatca aaaataattc
     3601 gcgtctggcc ttcctgtagc cagctttcat caacattaaa tgtgagcgag taacaacccg
     3661 tcggattctc cgtgggaaca aacggcggat tgaccgtaat gggataggtt acgttggtgt
     3721 agatgggcgc atcgtaaccg tgcatctgcc agtttgaggg gacgacgaca gtatcggcct
     3781 caggaagatc gcactccagc cagctttccg gcaccgcttc tggtgccgga aaccaggcaa
     3841 agcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     3901 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     3961 cagggttttc ccggtcgacc cgtaatctta cgtcagtaac ttccacagta gttcaccacc
     4021 ttttccctat ataaaagcat tagtgtatca acaagctggg gatcctagaa gcttatcgaa
     4081 ttctcatgtt tgacagctta tcatcggcaa tagttaccct tattatcaag ataagaaaga
     4141 aaaggatttt tcgctacgct caaatccttt aaaaaaacac aaaagaccac attttttaat
     4201 gtggtcttta ttcttcaact aaagcaccca ttagttcaac aaacgaaaat tggataaagt
     4261 gggatatttt taaaatatat atttatgtta cagtaatatt gacttttaaa aaaggattga
     4321 ttctaatgaa gaaagcagac aagtaagcct cctaaattca ctttagataa aaatttagga
     4381 ggcatatcaa atgaacttta ataaaattga tttagacaat tggaagagaa aagagatatt
     4441 taatcattat ttgaaccaac aaacgacttt tagtataacc acagaaattg atattagtgt
     4501 tttataccga aacataaaac aagaaggata taaattttac cctgcattta ttttcttagt
     4561 gacaagggtg ataaactcaa atacagcttt tagaactggt tacaatagcg acggagagtt
     4621 aggttattgg gataagttag agccacttta tacaattttt gatggtgtat ctaaaacatt
     4681 ctctggtatt tggactcctg taaagaatga cttcaaagag ttttatgatt tatacctttc
     4741 tgatgtagag aaatataatg gttcggggaa attgtttccc aaaacaccta tacctgaaaa
     4801 tgctttttct ctttctatta ttccatggac ttcatttact gggtttaact taaatatcaa
     4861 taataatagt aattaccttc tacccattat tacagcagga aaattcatta ataaaggtaa
     4921 ttcaatatat ttaccgctat ctttacaggt acatcattct gtttgtgatg gttatcatgc
     4981 aggattgttt atgaactcta ttcaggaatt gtcagatagg cctaatgact ggcttttata
     5041 atatgagata atgccgactg tactttttac agtcggtttt ctaatgtcac taacctgccc
     5101 cgttagttga agaaggtttt tatattacag ctccagatcc tctacgccgg acgcatcgtg
     5161 gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat
     5221 ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg
     5281 gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg
     5341 gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat
     5401 aagggagagc gtcgacatgg atgagcgatg atgatatccg tttaggctgg gcggtgatag
     5461 cttctcgttc aggcagtacg cctcttttct tttccagacc tgagggaggc ggaaatggtg
     5521 tgaggttccc ggggaaaagc caaataggcg atcgcgggag tgctttattt gaagatcagg
     5581 ctatcactgc ggtcaataga tttcacaatg tgatggctgg acagcctgag gaactctcga
     5641 acccgaatgg aaacaaccag atatttatga atcagcgcgg ctcacatggc gttgtgctgg
     5701 caaatgcagg ttcatcctct gtctctatca atacggcaac aaaattgcct gatggcaggt
     5761 atgacaataa agctggagcg ggttcatttc aagtgaacga tggtaaactg acaggcacga
     5821 tcaatgccag gtctgtagct gtgctttatc ctgatgatat tgcaaaagcg cctcatgttt
     5881 tccttgagaa ttacaaaaca ggtgtaacac attctttcaa tgatcaactg acgattacct
     5941 tgcgtgcaga tgcgaataca acaaaagccg tttatcaaat caataatgga ccagacgaca
     6001 ggcgtttaag gatggagatc aattcacaat cggaaaagga gatccaattt ggcaaaacat
     6061 acaccatcat gttaaaagga acgaacagtg atggtgtaac gaggaccgag aaatacagtt
     6121 ttgttaaaag agatccagcg tcggccaaaa ccatcggcta tcaaaatccg aatcattgga
     6181 gccaggtaaa tgcttatatc tataaacatg atgggagccg agtaattgaa ttgaccggat
     6241 cttggcctgg aaaaccaatg actaaaaatg cagacggaat ttacacgctg acgctgcctg
     6301 cggacacgga tacaaccaac gcaaaagtga tttttaataa tggcagcgcc caagtgcccg
     6361 gtcagaatca gcctggcttt gattacgtgc taaatggttt atataatgac tcgggcttaa
     6421 gcggttctct tccccattga gggcaaggct agacgggact taccgaaaga aaccatcaat
     6481 gatggtttct tttttgttca taaatcagac aaaacttttc tcttgcaaaa gtttgtgaag
     6541 tgttgcacaa tataaatgtg aaatacttca caaacaaaaa gacatcaaag agaaacatac
     6601 cctgcaagga tgctgatatt gtctgcattt gcgccggagc aaaccaaaaa cctggtgaga
     6661 cacgccttga attagtagaa aagaacttga agattttcaa aggcatcgtt agtgaagtca
     6721 tggcgagcgg atttgacggc attttcttag tcgcgacgcg aggctggatg gccttcccca
     6781 ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca
     6841 ggcaggtaga tgacgaccat cagggacagc ttcaaggatc gctcgcggct cttaccagcc
     6901 taacttcgat cactggaccg ctgatcgtca cggcgattta tgccgcctcg gcgagcacat
     6961 ggaacgggtt ggcatggatt gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc
     7021 gtcgcggtgc atggagccgg gccacctact gaagtggatt tctttaagag ctcctttaac
     7081 ttcctcacca gtagttgtat cggtaccata agtagaagca gcaacccaag tagctttacc
     7141 agcatccggt tcaaccagca tagtaagaat cttactggac atcggcagtt cttcgaacag
     7201 tgcgccaact accagctctt tctgcagttc attcagggca ccggagaacc tgcgtgcaat
     7261 ccatcttgtt caatcatgcg aaacgatcct catcctgtct cttgatccat ggattacgcg
     7321 ttaacccggg cccgcggatg catatgatca gatcttaagg cctaggtcta gaggatcgat
     7381 ctgtataata aagaataatt attaatctgt agacaaattg tgaaaggatg tacttaaacg
     7441 ctaacggtca gctttattga acagtaattt aagtatatgt ccaatctagg gtaagtaaat
     7501 tgagtatcaa tataaacttt atatgaacat aatcaacgag gtgaaatcat gagcaatttg
     7561 attaacggaa aaataccaaa tcaagcgatt caaacattaa aaatcgtaaa agatttattt
     7621 ggaagttcaa tagttggagt atatctattt ggttcagcag taaatggtgg tttacgcatt
     7681 aacagcgatg tagatgttct agtcgtcgtg aatcatagtt tacctcaatt aactcgaaaa
     7741 aaactaacag aaagactaat gactatatca ggaaagattg gaaatacgga ttctgttaga
     7801 ccacttgaag ttacggttat aaataggagt gaagttgtcc cttggcaata tcctccaaaa
     7861 agagaattta tatacggtga gtggctcagg ggtgaatttg agaatggaca aattcaggaa
     7921 ccaagctatg atcctgattt ggctattgtt ttagcacaag caagaaagaa tagtatttct
     7981 ctatttggtc ctgattcttc aagtatactt gtctccgtac ctttgacaga tattcgaaga
     8041 gcaattaagg attctttgcc agaactaatt gaggggataa aaggtgatga gcgtaatgta
     8101 attttaaccc tagctcgaat gtggcaaaca gtgactactg gtgaaattac ctcgaaagat
     8161 gtcgctgcag aatgggctat acctctttta cctaaagagc atgtaacttt actggatata
     8221 gctagaaaag gctatcgggg agagtgtgat gataagtggg aaggactata ttcaaaggtg
     8281 aaagcactcg ttaagtatat gaaaaattct atagaaactt ctctcaatta ggctaatttt
     8341 attgcaataa caggtgctta cttttctgga gttctttagc aaattttttt attagctgaa
     8401 cttagtatta gtggccatac tcctccaatc caaagctatt tagaaagatt actatatcct
     8461 caaacaggcg gtaaccggcc tcttcatcgg gaatgcgcgc gaccttcagc atcgccggca
     8521 tgtccccctg gcggacggga agtatccagc tcgaggtcgg gccgcgttgc tggcgttttt
     8581 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     8641 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     8701 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     8761 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     8821 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     8881 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     8941 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     9001 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     9061 cggaaaaaga gttgatagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     9121 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     9181 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     9241 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     9301 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     9361 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     9421 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     9481 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     9541 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     9601 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat
     9661 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     9721 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     9781 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     9841 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     9901 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga
     9961 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    10021 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    10081 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    10141 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    10201 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    10261 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    10321 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
    10381 cacgaggccc tttcgtcttc aagaatt
//
