LOCUS 40924_16415 3311 bp DNA circular SYN 01-JAN-1980 DEFINITION Vector for expressing rapidly maturing and noncytotoxic E2-Crimson in bacteria. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3311) AUTHORS Clontech TITLE Direct Submission REFERENCE 2 (bases 1 to 3311) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..3311 /mol_type="other DNA" /organism="synthetic DNA construct" protein_bind 107..128 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 143..173 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 181..197 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 205..221 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" CDS 289..963 /codon_start=1 /label=E2-Crimson /note="far-red noncytotoxic tetrameric variant of DsRed fluorescent protein (Strack et al., 2009)" /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGVGEGKPYEGTQTAK LQVTKGGPLPFAWDILSPQFFYGSKAYIKHPADIPDYLKQSFPEGFKWERVMNFEDGGV VTVTQDSSLQDGTLIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERNYPRDGVLKGENHM ALKLKGGGHYLCEFKSIYMAKKPVKLPGYHYVDYKLDITSHNEDYTVVEQYERAEARHH LFQ" promoter 1406..1510 /label=AmpR promoter CDS 1511..2368 /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 2542..3130 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg 241 catgcctgca ggtcgactct agaggatccc cgggtaccgg tcgccaccat ggatagcact 301 gagaacgtca tcaagccctt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc 361 cacgagttcg agatcgaggg cgtgggcgag ggcaagccct acgagggcac ccagaccgcc 421 aagctgcaag tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag 481 ttcttctacg gctccaaggc gtacatcaag caccccgccg acatccccga ctacctcaag 541 cagtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg 601 gtgaccgtga cccaggactc ctccctgcag gacggcaccc tcatctacca cgtgaagttc 661 atcggcgtga acttcccctc cgacggcccc gtaatgcaga agaagactct gggctgggag 721 ccctccactg agcgcaacta cccccgcgac ggcgtgctga agggcgagaa ccacatggcg 781 ctgaagctga agggcggcgg ccactacctg tgtgagttca agtccatcta catggccaag 841 aagcccgtga agctgcccgg ctaccactac gtggactaca agctcgacat cacctcccac 901 aacgaggact acaccgtggt ggagcagtac gagcgcgccg aggcccgcca ccacctgttc 961 cagtagcggc cgcgactcta gaattccaac tgagcgccgg tcgctaccat taccaacttg 1021 tctggtgtca aaaataatag gcctactagt cggccgtacg ggccctttcg tctcgcgcgt 1081 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt 1141 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg 1201 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg 1261 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg gccttaaggg 1321 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc 1381 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca 1441 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa 1501 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt 1561 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca 1621 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag 1681 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc 1741 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca 1801 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt 1861 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct 1921 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt 1981 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga 2041 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact 2101 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc 2161 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga 2221 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt 2281 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga 2341 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact 2401 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga 2461 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt 2521 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca 2581 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct 2641 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta 2701 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct 2761 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc 2821 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca 2881 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga 2941 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg 3001 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt 3061 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag 3121 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt 3181 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt 3241 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga 3301 ggaagcggaa g //