LOCUS       V042605                 8952 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042605
VERSION     V042605
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        123..1301
                     /note="strong constitutive promoter for human elongation
                     factor EF-1α"
                     /label="EF-1α promoter"
     CDS             1352..4909
                     /label="ERCC5(NM_000123)"
                     /note="ERCC5(NM_000123)"
                     /gene="ERCC5"
     CDS             4952..5671
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    5793..5914
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(5921..6376)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        6403..6507
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        6509..6866
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6901..7695
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    7927..7974
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      8303..8891
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 cgcgttgaca ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt
       61 tttaaacaga gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa
      121 ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
      181 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
      241 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
      301 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
      361 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
      421 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
      481 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
      541 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
      601 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
      661 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
      721 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
      781 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
      841 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
      901 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
      961 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     1021 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     1081 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     1141 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     1201 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     1261 cagtggttca aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtacgct
     1321 agcgtttaaa cttaagcttg gtaccgccac catgggggtc caggggctct ggaagctgct
     1381 ggagtgctcc gggcggcagg tcagccccga agcgctggaa gggaagatcc tggctgttga
     1441 tattagcatt tggttaaacc aagcacttaa aggagtccgg gatcgccatg ggaactcaat
     1501 agaaaatcct catcttctca ctttgtttca tcggctctgc aaactcttat tttttcgaat
     1561 tcgtcctatt tttgtgtttg atggggatgc tccactattg aagaaacaga ctttggtgaa
     1621 gagaaggcag agaaaggact tagcgtccag tgactccagg aaaacgacag agaagcttct
     1681 gaaaacattt ttgaaaagac aagccatcaa aactgccttc agaagcaaaa gagatgaagc
     1741 actacccagt cttacccaag ttcgaagaga aaacgacctc tatgttttgc ctcctttaca
     1801 agaggaagaa aaacacagtt cagaagagga agatgaaaaa gaatggcaag aaagaatgaa
     1861 tcaaaaacaa gcattacagg aagagttctt tcataatcct caagcgatag atattgagtc
     1921 tgaggacttc agcagcctgc cccctgaagt aaagcatgaa atcttgactg atatgaaaga
     1981 gttcaccaag cgcagaagaa cattatttga agcaatgcca gaggagtctg atgacttttc
     2041 acagtaccaa ctcaaaggct tgcttaaaaa gaactatctg aaccagcata tagaacatgt
     2101 ccaaaaggaa atgaatcagc aacattcagg acacatccga aggcagtatg aagatgaagg
     2161 gggctttctg aaggaggtag agtcaaggag agtggtctct gaagacactt cacattacat
     2221 cttgataaaa ggtattcaag ctaagacagt tgcagaagtg gattcagagt ctcttccttc
     2281 ttccagcaaa atgcacggca tgtcttttga cgtgaagtca tctccatgtg aaaaactgaa
     2341 gacagagaaa gagcctgatg ctacccctcc ttctccaaga actttactag ctatgcaagc
     2401 tgccctgctg ggaagtagct cagaagagga gctggagagt gaaaatcgaa ggcaggcccg
     2461 tgggaggaac gcacctgctg ctgtagacga aggctccata tcaccccgga ctctttcagc
     2521 cattaagaga gctcttgacg atgacgaaga tgtaaaagtg tgtgctgggg atgatgtgca
     2581 gacgggaggg ccaggagcag aagaaatgcg tataaacagc tccaccgaga acagtgatga
     2641 aggacttaaa gtgagagatg gaaaaggaat accgtttact gcaacacttg cgtcatctag
     2701 tgtgaactct gcagaggagc acgtagccag cactaatgag gggagagagc ccacagactc
     2761 agttccaaaa gaacaaatgt cacttgttca cgtggggact gaagcctttc cgataagtga
     2821 tgagtctatg attaaggaca gaaaagatcg gctgcctctg gagagtgcag tggttagaca
     2881 tagtgacgca cctgggctcc cgaatggaag ggaactgaca ccggcatctc caacttgtac
     2941 aaattctgtg tcaaagaatg aaacacatgc tgaagtgctt gagcagcaga acgaactttg
     3001 cccatatgag agtaaattcg attcttctct tctttcaagt gatgatgaaa caaaatgtaa
     3061 accgaattct gcttctgaag tcattggccc tgtcagtttg caagaaacaa gtagcatagt
     3121 aagtgtccct tcagaggcag tagataatgt ggaaaatgtg gtgtcattta atgctaaaga
     3181 gcatgagaat tttctggaaa ccatccaaga acagcagacc actgaatctg caggccagga
     3241 tttaatttcc attccaaagg ccgtggaacc aatggaaatt gactcggaag aaagtgaatc
     3301 tgatggaagt ttcattgaag tgcaaagtgt gattagtgat gaggaacttc aagcagaatt
     3361 ccctgaaact tccaaacctc cctcagaaca aggcgaagag gaactggtag gaactaggga
     3421 gggagaagcc cctgctgagt ccgagagcct cctgagggac aactctgaga gggacgacgt
     3481 ggatggtgag ccacaggaag ctgagaaaga tgcggaagat tcgctccatg aatggcaaga
     3541 tattaatttg gaggagttgg aaactctgga gagcaacctc ttagcacagc agaattcact
     3601 gaaagctcaa aaacagcagc aagaacggat cgctgctact gtcaccggac agatgttcct
     3661 ggaaagccag gaactcctgc gcctgttcgg cattccctac atccaggctc ccatggaagc
     3721 agaggcgcag tgcgccatcc tggacctgac tgatcagact tccggaacca tcactgatga
     3781 cagtgatatc tggctgtttg gagcgcggca tgtctataga aactttttta ataaaaacaa
     3841 gtttgtagaa tattatcaat atgtggactt tcacaatcaa ttgggattgg accggaataa
     3901 gttaataaat ttggcttatt tgcttggaag tgattatacc gaaggaatac caactgtggg
     3961 ttgtgtaacc gccatggaaa ttctcaatga attccctggg catggcctgg aacctctcct
     4021 aaaattctca gaatggtggc atgaagctca aaaaaatcca aagataagac ctaatcctca
     4081 tgacaccaaa gtgaaaaaaa aattacggac attgcaactc acccctggct ttcctaaccc
     4141 agctgttgcc gaggcctacc tcaaacccgt ggtggatgac tcgaagggat cctttctgtg
     4201 ggggaaacct gatctcgaca aaattagaga attttgtcag cggtatttcg gctggaacag
     4261 aacgaagaca gatgaatctc tgtttcctgt attaaagcaa ctcgatgccc agcagacaca
     4321 gctccgaatt gattccttct ttagattagc acaacaggag aaagaagatg ctaaacgtat
     4381 taagagccag agactaaaca gagctgtgac atgtatgcta aggaaagaga aagaagcagc
     4441 agccagcgaa atagaagcag tttctgttgc catggagaaa gaatttgagc tacttgataa
     4501 ggcaaaagga aaaacccaga agagaggcat aacaaatacc ttagaagagt catcaagcct
     4561 gaaaagaaag aggctttcag attctaaagg aaagaataca tgcggtggat ttttggggga
     4621 gacctgcctc tcagaatcat ctgatggatc ttcaagtgaa gatgctgaaa gttcatcttt
     4681 aatgaatgta caaaggagaa cagctgcgaa agagccaaaa accagtgctt cagattcgca
     4741 gaactcagtg aaggaagctc ccgtgaagaa tggaggtgcg accaccagca gctctagtga
     4801 tagtgatgac gatggaggga aagagaagat ggtcctcgtg accgccagat ctgtgtttgg
     4861 gaagaaaaga aggaaactaa gacgtgcgag gggaagaaaa aggaaaaccg gtggaggcgg
     4921 atcaggaggc ggtggatctg gatccgccac catggtgagc aagggcgagg agctgttcac
     4981 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     5041 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     5101 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     5161 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     5221 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     5281 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     5341 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     5401 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     5461 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     5521 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     5581 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     5641 cactctcggc atggacgagc tgtacaagta agcggccgcg actctagatc ataatcagcc
     5701 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     5761 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     5821 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     5881 gttgtggttt gtccaaactc atcaatgtat cttaaggcgt aaattgtaag cgttaatatt
     5941 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     6001 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     6061 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     6121 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     6181 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     6241 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     6301 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     6361 ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     6421 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     6481 cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt
     6541 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     6601 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     6661 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     6721 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     6781 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     6841 tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg atcgtttcgc
     6901 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     6961 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     7021 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     7081 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     7141 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     7201 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     7261 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     7321 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     7381 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac
     7441 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     7501 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     7561 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     7621 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     7681 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
     7741 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     7801 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     7861 cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg
     7921 ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcataaac
     7981 gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc
     8041 aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag
     8101 ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac tcatatatac
     8161 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     8221 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     8281 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     8341 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     8401 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     8461 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     8521 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     8581 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     8641 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     8701 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     8761 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     8821 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     8881 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     8941 ttgctcacat gt
//
