LOCUS V042603 6288 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V042603 VERSION V042603 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers promoter 123..1301 /note="strong constitutive promoter for human elongation factor EF-1α" /label="EF-1α promoter" CDS 1352..2245 /label="MPG(NM_002434)" /note="MPG(NM_002434)" /gene="MPG" CDS 2288..3007 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK*" /label="EGFP" polyA_signal 3129..3250 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(3257..3712) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3739..3843 /gene="bla" /label="AmpR promoter" promoter 3845..4202 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4237..5031 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5263..5310 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 5639..6227 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 cgcgttgaca ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt 61 tttaaacaga gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa 121 ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 181 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa 241 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 301 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg 361 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa 421 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt 481 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct 541 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg 601 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct 661 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg 721 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc 781 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc 841 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac 901 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga 961 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc 1021 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg 1081 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga 1141 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt 1201 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga 1261 cagtggttca aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtacgct 1321 agcgtttaaa cttaagcttg gtaccgccac catggtcacc cccgctttgc agatgaagaa 1381 accaaagcag ttttgccgac ggatggggca aaagaagcag cgaccagcta gagcagggca 1441 gccacacagc tcgtccgacg cagcccaggc acctgcagag cagccacaca gctcgtccga 1501 tgcagcccag gcaccttgcc ccagggagcg ctgcttggga ccgcccacca ctccgggccc 1561 ataccgcagc atctatttct caagcccaaa gggccacctt acccgactgg ggttggagtt 1621 cttcgaccag ccggcagtcc ccctggcccg ggcatttctg ggacaggtcc tagtccggcg 1681 acttcctaat ggcacagaac tccgaggccg catcgtggag accgaggcat acctggggcc 1741 agaggatgaa gccgcccact caaggggtgg ccggcagacc ccccgcaacc gaggcatgtt 1801 catgaagccg gggaccctgt acgtgtacat catttacggc atgtacttct gcatgaacat 1861 ctccagccag ggggacgggg cttgcgtctt gctgcgagca ctggagcccc tggaaggtct 1921 ggagaccatg cgtcagcttc gcagcaccct ccggaaaggc accgccagcc gtgtcctcaa 1981 ggaccgcgag ctctgcagtg gcccctccaa gctgtgccag gccctggcca tcaacaagag 2041 ctttgaccag agggacctgg cacaggatga agctgtatgg ctggagcgtg gtcccctgga 2101 gcccagtgag ccggctgtag tggcagcagc ccgggtgggc gtcggccatg caggggagtg 2161 ggcccggaaa cccctccgct tctatgtccg gggcagcccc tgggtcagtg tggtcgacag 2221 agtggctgag caggacacac aggccggtgg aggcggatca ggaggcggtg gatctggatc 2281 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga 2341 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc 2401 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg 2461 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca 2521 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac 2581 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga 2641 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct 2701 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca 2761 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca 2821 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga 2881 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca 2941 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta 3001 caagtaagcg gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac 3061 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg 3121 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 3181 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 3241 atgtatctta aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt 3301 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca 3361 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta 3421 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta 3481 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg 3541 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga 3601 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg 3661 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc 3721 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat 3781 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag 3841 agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc 3901 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt 3961 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 4021 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg 4081 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct 4141 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca 4201 aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac 4261 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca 4321 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt 4381 gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg 4441 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga 4501 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct 4561 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg 4621 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg 4681 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc 4741 gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat 4801 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac 4861 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt 4921 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct 4981 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc 5041 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca 5101 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga 5161 tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg 5221 aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga 5281 ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc 5341 actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt 5401 ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg 5461 caggccctgc catagcctca ggttactcat atatacttta gattgattta aaacttcatt 5521 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 5581 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 5641 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 5701 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 5761 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca 5821 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 5881 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 5941 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 6001 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 6061 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 6121 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 6181 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 6241 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt //