LOCUS       V033739                 8760 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033739
VERSION     V033739
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        123..1301
                     /note="strong constitutive promoter for human elongation
                     factor EF-1α"
                     /label="EF-1α promoter"
     CDS             1352..4006
                     /label="Pml(NM_178087)"
                     /note="Pml(NM_178087)"
                     /gene="Pml"
     CDS             4037..5467
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="tandem dimeric (pseudo-monomeric) derivative of
                     DsRed (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK*"
                     /label="tdTomato"
     polyA_signal    5601..5722
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(5729..6184)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        6211..6315
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        6317..6674
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6709..7503
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    7735..7782
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      8111..8699
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 cgcgttgaca ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt
       61 tttaaacaga gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa
      121 ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
      181 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
      241 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
      301 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
      361 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
      421 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
      481 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
      541 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
      601 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
      661 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
      721 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
      781 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
      841 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
      901 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
      961 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     1021 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     1081 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     1141 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     1201 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     1261 cagtggttca aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtacgct
     1321 agcgtttaaa cttaagcttg gtaccgccac catggaaact gaaccagttt ccgtgcagaa
     1381 ggtacctgca ccccctggat ctccctgtcg acaacaggac tctgccctga cccccacgcc
     1441 caccatgcct cccccagagg aaccctccga agactatgaa cacagccaaa gccctgcaga
     1501 gcaagccata caggaggaat ttcagtttct gcgctgcccg agctgccagg cccaagccaa
     1561 gtgccccaaa ctgctgcctt gcctgcacac gctgtgctcc ggatgcctgg aggcgcctgg
     1621 cctgcagtgc cccatctgca aggctcctgg gcaggccgat gctaatgggg aggccctgga
     1681 taacgtgttc ttcgagagcc tgcagcggcg cctggcggtg ttccggcaga tcgtggatgc
     1741 tcaggctgcg tgcacccgct gcaaaggctt ggccgacttc tggtgtttcg agtgtgaaca
     1801 gctcatttgc agcaagtgct tcgaagcaca ccagtggtac ctcaagcatg aggcccggcc
     1861 cctggctgat ctccgcgaca attcagtgag cagcttcctc gacagtacgc gcaagtccaa
     1921 tatcttctgc tccaatacca accaccgcaa ccctgcgctg actgacatct actgccgagg
     1981 ctgcgccaag cctctgtgtt gcacatgcgc gctcctggac cgcaaccaca gccatctcca
     2041 ttgcgatatt ggtgaggaga ttcagcagtg gcatgaggaa ctaggcacca tgacacagac
     2101 tctggaggag cagggcagaa ccttcgacag tgcccatgca cagatgtgct cagctatagg
     2161 acagctggac cacgcacgcg cagacattga gaagcagatc cgcgcacgcg tgcgccaggt
     2221 ggtagactac gtgcaggctc aggagcgcga gctgctcgag gcggtgaatg accgctacca
     2281 gcgcgactac caggaaatag ctggccagct gagctgcctg gaagctgtgc tgcagcgcat
     2341 ccgcactagt ggggcgctgg tcaagaggat gaagctctat gcctccgacc aggaggtgct
     2401 ggatatgcac agctttctgc gcaaggcact ctgtagcttg cgccaggagg agccccagaa
     2461 ccagaaagtc cagctgctca ccagaggttt cgaggagttc aagctgtgcc tgcaggactt
     2521 catctcctgc atcacccaga ggataaatgc agctgtagcc agcccagagg cagccagcaa
     2581 tcaaccagag gcagccagca ctcacccagt gacaaccagc acgcctgagg accttgagca
     2641 gcccaaagaa gtgcagagtg tacaggctca ggccctagag ctgtctaaga cccaacctgt
     2701 ggctatggta aaaacagtgc ctggagcaca ccctgtacca gtgtatgcct tttcaatgca
     2761 aggccccacc tacagagagg aggcttctca gacagtcggc tccatgaaga ggaagtgctc
     2821 ccacgaagat tgctccagga agatcatcaa gatggagtcc acagaggaga acgaggacag
     2881 gttggccaca agctcccccg agcagtcctg gcccagcact ttcaaggcca cctcccctcc
     2941 ccatctggat gggacttcca accctgagag caccgtccct gaaaaaaaga tcctcctgcc
     3001 caacaacaat catgttacca gtgacacagg ggaaacagag gagcgagttg tggtgatcag
     3061 cagctcagag gattcggaca ccgagaatct gtcctcccac gagttggacg atagcagcag
     3121 tgagtccagc agcctgcagc tggagggccc caattccctc aaggccttgg atgagagcct
     3181 cgccgaaccc cacttagaag acaggaccct ggttttcttt gacctcaaga ttgacaatga
     3241 aacccagaaa attagccagc tggccgcggt gaaccgggaa agcaagttcc gtgtgctcat
     3301 ccagcccgag gccttcagtg tctactccaa agctgtctcc ctggaggcgg ggctccggca
     3361 cttcctcagc ttcctcacca ccatgcaccg tcccatcttg gcgtgttcta ggctgtgggg
     3421 gccgggactc cccatcttct tccagaccct gagtgatatt aacaagctgt gggaattcca
     3481 ggacaccatc tcaggtttct tggccgtgct gcccctcatc cgggaacgca tacccggcgc
     3541 tagcagcttc aaacttggga acctagccaa gacctacctg gcgagaaaca tgagcgaacg
     3601 cagcgctctg gcttctgtgc tggccatgag ggacttgtgc tgcctccttg agatctcccc
     3661 aggactgccg ctggcccagc atatctactc ctttagtagc ttgcagtgtt ttgcttccct
     3721 gcagccccta attcaggcca gcgtcctgcc acagtccgag gcccgcctct tggccctcca
     3781 caatgtgagc tttgtagagt tactgaatgc atatcgcacc aacaggcaag aaggcttgaa
     3841 gaagtatgtc cactatctga gcctgcagac caccccgttg tcatcgtcgg cttccaccca
     3901 agttgcccaa ttcctgcagg ctcttagcac ccacatggaa ggactgttgg aaggccatgc
     3961 ccctgctggg gcagaaggca aagctgaaag taagggatgc ctggccggtg gaggcggatc
     4021 aggaggcggt ggatccatgg tgagcaaggg cgaggaggtc atcaaagagt tcatgcgctt
     4081 caaggtgcgc atggagggct ccatgaacgg ccacgagttc gagatcgagg gcgagggcga
     4141 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct
     4201 gcccttcgcc tgggacatcc tgtcccccca gttcatgtac ggctccaagg cgtacgtgaa
     4261 gcaccccgcc gacatccccg attacaagaa gctgtccttc cccgagggct tcaagtggga
     4321 gcgcgtgatg aacttcgagg acggcggtct ggtgaccgtg acccaggact cctccctgca
     4381 ggacggcacg ctgatctaca aggtgaagat gcgcggcacc aacttccccc ccgacggccc
     4441 cgtaatgcag aagaagacca tgggctggga ggcctccacc gagcgcctgt acccccgcga
     4501 cggcgtgctg aagggcgaga tccaccaggc cctgaagctg aaggacggcg gccactacct
     4561 ggtggagttc aagaccatct acatggccaa gaagcccgtg caactgcccg gctactacta
     4621 cgtggacacc aagctggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     4681 cgagcgctcc gagggccgcc accacctgtt cctggggcat ggcaccggca gcaccggcag
     4741 cggcagctcc ggcaccgcct cctccgagga caacaacatg gccgtcatca aagagttcat
     4801 gcgcttcaag gtgcgcatgg agggctccat gaacggccac gagttcgaga tcgagggcga
     4861 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg
     4921 ccccctgccc ttcgcctggg acatcctgtc cccccagttc atgtacggct ccaaggcgta
     4981 cgtgaagcac cccgccgaca tccccgatta caagaagctg tccttccccg agggcttcaa
     5041 gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg accgtgaccc aggactcctc
     5101 cctgcaggac ggcacgctga tctacaaggt gaagatgcgc ggcaccaact tcccccccga
     5161 cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc
     5221 ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg aagctgaagg acggcggcca
     5281 ctacctggtg gagttcaaga ccatctacat ggccaagaag cccgtgcaac tgcccggcta
     5341 ctactacgtg gacaccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga
     5401 acagtacgag cgctccgagg gccgccacca cctgttcctg tacggcatgg acgagctgta
     5461 caagtaggga tcctcagacg cggccgcgac tctagatcat aatcagccat accacatttg
     5521 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     5581 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     5641 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     5701 ccaaactcat caatgtatct taaggcgtaa attgtaagcg ttaatatttt gttaaaattc
     5761 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     5821 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     5881 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     5941 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     6001 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     6061 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     6121 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     6181 gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     6241 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     6301 tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt
     6361 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     6421 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     6481 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     6541 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     6601 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     6661 taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
     6721 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     6781 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     6841 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca
     6901 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     6961 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     7021 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     7081 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     7141 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     7201 ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc
     7261 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     7321 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     7381 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     7441 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     7501 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
     7561 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     7621 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg
     7681 ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa
     7741 taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt
     7801 cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg
     7861 tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca
     7921 acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt tagattgatt
     7981 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     8041 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     8101 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     8161 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     8221 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     8281 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     8341 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     8401 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     8461 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     8521 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     8581 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     8641 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     8701 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
