LOCUS       pENTR1A.        3754 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Gateway(R) Dual Selection Vector for creating an entry vector by 
            restriction cloning. Other reading frames are available with 
            pENTR(TM)2B and pENTR(TM)3C.
ACCESSION   .
VERSION     .
KEYWORDS    pENTR1A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     To create a Kozak sequence, clone a blunt fragment starting with 
            ATGG (where ATG is the start codon) into the XmnI site.
FEATURES             Location/Qualifiers
     source          1..3754
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     protein_bind    358..457
                     /label=attL1
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    457..507
                     /label=MCS 1
                     /note="MCS 1"
                     /note="multiple cloning site, part 1"
     RBS             461..466
                     /note="ribosome binding site"
     promoter        524..554
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             608..1285
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             1608..1910
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     misc_feature    1948..1979
                     /label=MCS 2
                     /note="MCS 2"
                     /note="multiple cloning site, part 2"
     protein_bind    complement(1983..2082)
                     /label=attL2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2205..3011
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      3104..3692
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggcttta aaggaaccaa ttcagtcgac
      481 tggatccggt accgaattcg cggccgcatt aggcacccca ggctttacac tttatgcttc
      541 cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg agattttcag gagctaagga
      601 agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg
      661 taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca
      721 gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc
      781 ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattccgta tggcaatgaa
      841 agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca
      901 aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca
      961 catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt
     1021 tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt
     1081 aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac
     1141 gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg tttgtgatgg
     1201 cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcaggcggg
     1261 gcgtaatcta gaggatccgg cttactaaaa gccagataac agtatgcgta tttgcgcgct
     1321 gatttttgcg gtataagaat atatactgat atgtataccc gaagtatgtc aaaaagaggt
     1381 atgctatgaa gcagcgtatt acagtgacag ttgacagcga cagctatcag ttgctcaagg
     1441 catatatgat gtcaatatct ccggtctggt aagcacaacc atgcagaatg aagcccgtcg
     1501 tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt
     1561 tattgaaatg aacggctctt ttgctgacga gaacaggggc tggtgaaatg cagtttaagg
     1621 tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag agtgatatta
     1681 ttgacacgcc cgggcgacgg atggtgatcc ccctggccag tgcacgtctg ctgtcagata
     1741 aagtcccccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg cgcatgatga
     1801 ccaccgatat ggccagtgtg ccggtctccg ttatcgggga agaagtggct gatctcagcc
     1861 accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata taaatgtcag
     1921 gctcccttat acacagccag tctgcaggtc gacgaattcg cggccgcact cgagatatct
     1981 agacccagct ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg
     2041 caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag ctgcagctct
     2101 ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa
     2161 caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac
     2221 gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat
     2281 gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg
     2341 atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg
     2401 agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta
     2461 tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc
     2521 aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgtccc
     2581 tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc
     2641 gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg
     2701 acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat
     2761 tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg
     2821 aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg
     2881 atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt
     2941 ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg
     3001 atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ttattcagat tgggccccgt
     3061 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     3121 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     3181 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     3241 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     3301 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     3361 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     3421 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     3481 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     3541 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     3601 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     3661 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     3721 tcctggcctt ttgctggcct tttgctcaca tgtt
//
