LOCUS Exported 6706 bp ds-DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pESC-HIS, complete sequence. ACCESSION AF063850 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6706) AUTHORS Lu Q. TITLE pESC-HIS JOURNAL Unpublished REFERENCE 2 (bases 1 to 6706) AUTHORS Lu Q. TITLE Direct Submission JOURNAL Submitted (08-MAY-1998) Technical Services, Stratagene, 11011 N. Torrey Pines Rd., La Jolla, CA 92037, USA REFERENCE 3 (bases 1 to 6706) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..6706 /organism="Cloning vector pESC-HIS" /host="Escherichia coli K12" /mol_type="genomic DNA" /db_xref="taxon:76667" promoter 317..503 /gene="S. cerevisiae HIS3" /label=HIS3 promoter CDS 504..1163 /codon_start=1 /gene="S. cerevisiae HIS3" /product="imidazoleglycerol-phosphate dehydratase, required for histidine biosynthesis" /label=HIS3 /note="yeast auxotrophic marker" /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF KEALLARGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHFL ESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM" rep_origin complement(1423..1878) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" terminator 1961..2126 /gene="S. cerevisiae ADH1" /label=ADH1 terminator /note="transcription terminator for the S. cerevisiae alcohol dehydrogenase 1 (ADH1) gene" CDS complement(2274..2297) /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" promoter 2344..3008 /gene="GAL1 and GAL10 genes of S. cerevisiae" /label=GAL1,10 promoter /note="divergent inducible promoter, regulated by Gal4" protein_bind 2560..2677 /label=UAS /bound_moiety="Gal4" /note="upstream activating sequence mediating Gal4-dependent induction" promoter 3019..3037 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 3055..3084 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" terminator 3114..3303 /gene="S. cerevisiae CYC1" /label=CYC1 terminator /note="transcription terminator for CYC1" rep_origin complement(3546..4134) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4305..5165) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(5166..5270) /gene="bla" /label=AmpR promoter rep_origin 5297..6639 /label=2u ori /note="yeast 2u plasmid origin of replication" protein_bind complement(6260..6307) /label=FRT /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accataaatt cccgttttaa gagcttggtg agcgctagga gtcactgcca ggtatcgttt 241 gaacacggca ttagtcaggg aagtcataac acagtccttt cccgcaattt tctttttcta 301 ttactcttgg cctcctctag tacactctat atttttttat gcctcggtaa tgattttcat 361 tttttttttt cccctagcgg atgactcttt ttttttctta gcgattggca ttatcacata 421 atgaattata cattatataa agtaatgtga tttcttcgaa gaatatacta aaaaatgagc 481 aggcaagata aacgaaggca aagatgacag agcagaaagc cctagtaaag cgtattacaa 541 atgaaaccaa gattcagatt gcgatctctt taaagggtgg tcccctagcg atagagcact 601 cgatcttccc agaaaaagag gcagaagcag tagcagaaca ggccacacaa tcgcaagtga 661 ttaacgtcca cacaggtata gggtttctgg accatatgat acatgctctg gccaagcatt 721 ccggctggtc gctaatcgtt gagtgcattg gtgacttaca catagacgac catcacacca 781 ctgaagactg cgggattgct ctcggtcaag cttttaaaga ggccctactg gcgcgtggag 841 taaaaaggtt tggatcagga tttgcgcctt tggatgaggc actttccaga gcggtggtag 901 atctttcgaa caggccgtac gcagttgtcg aacttggttt gcaaagggag aaagtaggag 961 atctctcttg cgagatgatc ccgcattttc ttgaaagctt tgcagaggct agcagaatta 1021 ccctccacgt tgattgtctg cgaggcaaga atgatcatca ccgtagtgag agtgcgttca 1081 aggctcttgc ggttgccata agagaagcca cctcgcccaa tggtaccaac gatgttccct 1141 ccaccaaagg tgttcttatg tagtgacacc gattatttaa agctgcagca tacgatatat 1201 atacatgtgt atatatgtat acctatgaat gtcagtaagt atgtatacga acagtatgat 1261 actgaagatg acaaggtaat gcatcattct atacgtgtca ttctgaacga ggcgcgcttt 1321 ccttttttct ttttgctttt tctttttttt tctcttgaac tcgacggatc tatgcggtgt 1381 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta aacgttaata 1441 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg 1501 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc 1561 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa 1621 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt 1681 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac 1741 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta 1801 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg 1861 cgccgctaca gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt tgggaagggc 1921 gatcggtgcg ggcctcttcg ctattacgcc agctgaattg gagcgacctc atgctatacc 1981 tgagaaagca acctgaccta caggaaagag ttactcaaga ataagaattt tcgttttaaa 2041 acctaagagt cactttaaaa tttgtataca cttatttttt ttataactta tttaataata 2101 aaaatcataa atcataagaa attcgcttat ttagaagtgt caacaacgta tctaccaacg 2161 atttgaccct tttccatctt ttcgtaaatt tctggcaagg tagacaagcc gacaaccttg 2221 attggagact tgaccaaacc tctggcgaag aattgttaat taagagctca gatcttatcg 2281 tcgtcatcct tgtaatccat cgatactagt gcggccgccc tttagtgagg gttgaattcg 2341 aattttcaaa aattcttact ttttttttgg atggacgcaa agaagtttaa taatcatatt 2401 acatggcatt accaccatat acatatccat atacatatcc atatctaatc ttacttatat 2461 gttgtggaaa tgtaaagagc cccattatct tagcctaaaa aaaccttctc tttggaactt 2521 tcagtaatac gcttaactgc tcattgctat attgaagtac ggattagaag ccgccgagcg 2581 ggtgacagcc ctccgaagga agactctcct ccgtgcgtcc tcgtcttcac cggtcgcgtt 2641 cctgaaacgc agatgtgcct cgcgccgcac tgctccgaac aataaagatt ctacaatact 2701 agcttttatg gttatgaaga ggaaaaattg gcagtaacct ggccccacaa accttcaaat 2761 gaacgaatca aattaacaac cataggatga taatgcgatt agttttttag ccttatttct 2821 ggggtaatta atcagcgaag cgatgatttt tgatctatta acagatatat aaatgcaaaa 2881 actgcataac cactttaact aatactttca acattttcgg tttgtattac ttcttattca 2941 aatgtaataa aagtatcaac aaaaaattgt taatatacct ctatacttta acgtcaagga 3001 gaaaaaaccc cggatccgta atacgactca ctatagggcc cgggcgtcga catggaacag 3061 aagttgattt ccgaagaaga cctcgagtaa gcttggtacc gcggctagct aagatccgct 3121 ctaaccgaaa aggaaggagt tagacaacct gaagtctagg tccctattta tttttttata 3181 gttatgttag tattaagaac gttatttata tttcaaattt ttcttttttt tctgtacaga 3241 cgcgtgtacg catgtaacat tatactgaaa accttgcttg agaaggtttt gggacgctcg 3301 aagatccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 3361 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 3421 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 3481 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 3541 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 3601 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 3661 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 3721 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 3781 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 3841 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 3901 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 3961 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca 4021 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 4081 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 4141 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 4201 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 4261 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 4321 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 4381 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 4441 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 4501 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 4561 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 4621 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 4681 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 4741 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 4801 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 4861 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 4921 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 4981 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 5041 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 5101 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 5161 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 5221 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 5281 cgaaaagtgc cacctgaacg aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc 5341 gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa 5401 cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc 5461 aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa 5521 tgcaacgcga gagcgctatt ttaccaacaa agaatctata cttctttttt gttctacaaa 5581 aatgcatccc gagagcgcta tttttctaac aaagcatctt agattacttt ttttctcctt 5641 tgtgcgctct ataatgcagt ctcttgataa ctttttgcac tgtaggtccg ttaaggttag 5701 aagaaggcta ctttggtgtc tattttctct tccataaaaa aagcctgact ccacttcccg 5761 cgtttactga ttactagcga agctgcgggt gcattttttc aagataaagg catccccgat 5821 tatattctat accgatgtgg attgcgcata ctttgtgaac agaaagtgat agcgttgatg 5881 attcttcatt ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta tatactacgt 5941 ataggaaatg tttacatttt cgtattgttt tcgattcact ctatgaatag ttcttactac 6001 aatttttttg tctaaagagt aatactagag ataaacataa aaaatgtaga ggtcgagttt 6061 agatgcaagt tcaaggagcg aaaggtggat gggtaggtta tatagggata tagcacagag 6121 atatatagca aagagatact tttgagcaat gtttgtggaa gcggtattcg caatatttta 6181 gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc agagcgcttt 6241 tggttttcaa aagcgctctg aagttcctat actttctaga gaataggaac ttcggaatag 6301 gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag ctgcgcacat 6361 acagctcact gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat atatacatga 6421 gaagaacggc atagtgcgtg tttatgctta aatgcgtact tatatgcgtc tatttatgta 6481 ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg ggtatcgtat 6541 gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa ttggattagt 6601 ctcatccttc aatgctatca tttcctttga tattggatca tctaagaaac cattattatc 6661 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc //