LOCUS Exported 3355 bp ds-DNA circular SYN 04-1-2013 DEFINITION Vector for expressing EYFP in bacteria. ACCESSION . VERSION . KEYWORDS pEYFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3355) AUTHORS Clontech TITLE Direct Submission COMMENT Translation from the lacZ start codon produces a fusion protein in E. coli. FEATURES Location/Qualifiers source 1..3355 /organism="synthetic DNA construct" /lab_host="Escherichia coli" /mol_type="other DNA" promoter 143..173 /note="lac promoter" /note="promoter for the E. coli lac operon" protein_bind 181..197 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 205..221 /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" CDS 217..219 /codon_start=1 /product="lacZ start codon" /note="ATG" /translation="M" CDS 289..1008 /codon_start=1 /product="enhanced YFP" /note="EYFP" /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" promoter 1450..1554 /gene="bla" /note="AmpR promoter" CDS 1555..2415 /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 2586..3174 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg 241 catgcctgca ggtcgactct agaggatccc cgggtaccgg tcgccaccat ggtgagcaag 301 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac 361 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc 421 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc 481 ttcggctacg gcctgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc 541 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac 601 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc 661 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac 721 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg 781 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag 841 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac 901 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc 961 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaag cggccgcgac 1021 tctagaattc caactgagcg ccggtcgcta ccattaccaa cttgtctggt gtcaaaaata 1081 ataggcctac tagtcggccg tacgggccct ttcgtctcgc gcgtttcggt gatgacggtg 1141 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg 1201 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta 1261 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc 1321 acagatgcgt aaggagaaaa taccgcatca ggcggcctta agggcctcgt gatacgccta 1381 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg 1441 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 1501 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt 1561 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt 1621 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg 1681 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa 1741 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt 1801 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag 1861 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 1921 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 1981 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt 2041 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta 2101 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 2161 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 2221 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 2281 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 2341 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 2401 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 2461 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 2521 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 2581 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 2641 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 2701 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac 2761 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 2821 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 2881 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 2941 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 3001 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 3061 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 3121 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 3181 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 3241 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc 3301 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaag //