LOCUS V019938 8393 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V019938 VERSION V019938 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 683..3073 /label="ADGRG7(NM_032787)" /note="ADGRG7(NM_032787)" /gene="ADGRG7" CDS 3080..3145 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" misc_feature 3169..3757 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 3823..3871 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 4073..4501 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4515..4844 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4911..5705 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5881..6002 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(6051..6067) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6075..6091 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6099..6129) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6144..6165 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6453..7041) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7212..8072) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8073..8177) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg 661 taccgagctc ggatccgcca ccatggcttc ctgccgtgcc tggaacctta gggtgctggt 721 ggctgtcgtg tgtggactac tgactggcat cattttggga ctgggcatct ggaggattgt 781 gatcaggatc caaagaggaa aatctacttc ctcatcaagc acccctacag agttctgcag 841 gaatggtgga acctgggaaa atggcagatg tatttgtaca gaagagtgga aaggactgag 901 atgtacaatt gctaattttt gtgaaaatag tacctatatg ggttttactt ttgccagaat 961 cccagtgggc agatatggac catccttgca aacatgtggc aaggatactc caaatgcggg 1021 caatccaatg gcagtccggt tgtgcagtct ctctctatat ggagagatag aattacaaaa 1081 agtgacaata ggaaattgca atgaaaatct ggaaaccctg gaaaagcagg taaaggatgt 1141 cacagcacca cttaataaca tttcttctga agtccagatt ttaacatctg atgccaataa 1201 attaactgct gagaacatca ctagtgctac gcgagtggtt ggacagatat tcaacacttc 1261 cagaaatgct tcacctgagg caaagaaagt tgccatagta acagtgagtc aactcctaga 1321 tgccagtgaa gatgcttttc aaagagttgc tgctactgct aatgatgatg cccttacaac 1381 gcttattgag caaatggaga cttattcctt gtctttgggt aatcaatcag tggtggaacc 1441 taacatagca atacagtcag caaatttctc ttcagaaaat gcggtggggc cttcaaatgt 1501 tcgcttctct gtgcagaaag gagctagcag ttctctagtt tctagttcaa catttataca 1561 tacaaatgtg gatggcctta acccagatgc acagactgag cttcaggtct tgcttaatat 1621 gacgaaaaat tacaccaaga catgcggctt tgtagtttat caaaatgaca agcttttcca 1681 atcaaaaact tttacagcta aatcggattt tagtcaaaaa attatctcaa gcaaaactga 1741 tgaaaatgag caagatcaga gtgcttctgt tgacatggtc tttagtccaa agtacaacca 1801 aaaagaattt caactctatt cctatgcctg tgtctattgg aatttgtcag cgaaggactg 1861 ggacacatat ggctgtcaaa aagacaaggg cactgatgga ttcctgcgct gccgctgcaa 1921 ccatactact aattttgctg tattaatgac tttcaaaaag gattatcaat atcccaaatc 1981 acttgacata ttatccaacg ttggatgtgc actgtctgtt actggtctgg ctctcacagt 2041 tatatttcag attgtcacca ggaaagtcag aaaaacctca gtaacctggg ttttggtcaa 2101 tctgtgcata tcaatgttga ttttcaacct cctctttgtg tttggaattg aaaactccaa 2161 taagaacttg cagacaagtg atggtgacat caataatatt gactttgaca ataatgacat 2221 acccaggaca gacaccatta acatcccgaa tcccatgtgc actgcgattg ccgccttact 2281 gcactatttt ctgttagtga catttacctg gaacgcactc agcgctgcac agctctatta 2341 ccttctaata aggaccatga agcctcttcc tcggcatttc attcttttca tctcattaat 2401 tggatgggga gtcccagcta tagtagtggc tataacagtg ggagttattt attctcagaa 2461 tggaaataat ccacagtggg aattagacta ccggcaagag aaaatctgct ggctggcaat 2521 tccagaaccc aatggtgtta taaaaagtcc gctgttgtgg tcattcatcg tacctgtaac 2581 cattatcctc atcagcaatg ttgttatgtt tattacaatc tcgatcaaag tgctgtggaa 2641 gaataaccag aacctgacaa gcacaaaaaa agtttcatcc atgaagaaga ttgttagcac 2701 attatctgtt gcagttgttt ttggaattac ctggattcta gcatacctga tgctagttaa 2761 tgatgatagc atcaggatcg tcttcagcta catattctgc cttttcaaca ctacacaggg 2821 attgcaaatt tttatcctgt acactgttag aacaaaagtc ttccagagtg aagcttccaa 2881 agtgttgatg ttgctatcgt ctattgggag aaggaagtca ttgccttcag tgacgcggcc 2941 gaggctgcgt gtaaagatgt ataatttcct caggtcattg ccaaccttac atgaacgctt 3001 taggctactg gaaacctctc cgagtactga ggaaatcaca ctctctgaaa gtgacaatgc 3061 aaaggaaagc atcctcgagg actacaaaga ccatgacggt gattataaag atcatgacat 3121 cgactacaag gatgacgatg acaagtagtg agggcccgat atctcgacaa tcaacctctg 3181 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta 3241 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt 3301 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc 3361 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt 3421 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg 3481 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac 3541 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc 3601 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac 3661 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct 3721 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaaacggggg aggctaactg 3781 aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga 3841 ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg 3901 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt 3961 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg 4021 gcaggccctg ccatagcaga tctgcgcagc tggggctcta gggggtatcc ccacgcgccc 4081 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt 4141 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc 4201 ggctttcccc gtcaagctct aaatcggggc atccctttag ggttccgatt tagtgcttta 4261 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc 4321 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg 4381 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt 4441 ttggggattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat 4501 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca 4561 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct 4621 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc 4681 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg 4741 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc 4801 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt 4861 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac 4921 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact 4981 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc 5041 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg 5101 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg 5161 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt 5221 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc 5281 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag 5341 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg 5401 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc 5461 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt 5521 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg 5581 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt 5641 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct 5701 tctgagcggg actctggggt tcgcgaaatg accgaccaag cgacgcccaa cctgccatca 5761 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg 5821 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc 5881 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 5941 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 6001 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg 6061 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 6121 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca 6181 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc 6241 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg 6301 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 6361 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 6421 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 6481 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 6541 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 6601 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt 6661 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 6721 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 6781 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 6841 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta 6901 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 6961 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 7021 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 7081 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 7141 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 7201 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 7261 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 7321 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 7381 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 7441 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 7501 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 7561 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 7621 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 7681 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 7741 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 7801 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 7861 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 7921 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 7981 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 8041 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 8101 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 8161 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga 8221 gatctcccga tcccctatgg tcgactctca gtacaatctg ctctgatgcc gcatagttaa 8281 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt 8341 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agg //