LOCUS V041217 7626 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V041217 VERSION V041217 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 678..696 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 756..2306 /label="ALDH2(NM_000690)" /note="ALDH2(NM_000690)" /gene="ALDH2" CDS 2313..2378 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" misc_feature 2402..2990 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 3056..3104 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 3306..3734 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3748..4077 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4144..4938 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5114..5235 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5284..5300) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5308..5324 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5332..5362) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5377..5398 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5686..6274) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6445..7305) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7306..7410) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct 661 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta 721 aacttaagct tggtaccgag ctcgaattcg ccaccatgtt gcgcgctgcc gcccgcttcg 781 ggccccgcct gggccgccgc ctcttgtcag ccgccgccac ccaggccgtg cctgccccca 841 accagcagcc cgaggtcttc tgcaaccaga ttttcataaa caatgaatgg cacgatgccg 901 tcagcaggaa aacattcccc accgtcaatc cgtccactgg agaggtcatc tgtcaggtag 961 ctgaagggga caaggaagat gtggacaagg cagtgaaggc cgcccgggcc gccttccagc 1021 tgggctcacc ttggcgccgc atggacgcat cacacagggg ccggctgctg aaccgcctgg 1081 ccgatctgat cgagcgggac cggacctacc tggcggcctt ggagaccctg gacaatggca 1141 agccctatgt catctcctac ctggtggatt tggacatggt cctcaaatgt ctccggtatt 1201 atgccggctg ggctgataag taccacggga aaaccatccc cattgacgga gacttcttca 1261 gctacacacg ccatgaacct gtgggggtgt gcgggcagat cattccgtgg aatttcccgc 1321 tcctgatgca agcatggaag ctgggcccag ccttggcaac tggaaacgtg gttgtgatga 1381 aggtagctga gcagacaccc ctcaccgccc tctatgtggc caacctgatc aaggaggctg 1441 gctttccccc tggtgtggtc aacattgtgc ctggatttgg ccccacggct ggggccgcca 1501 ttgcctccca tgaggatgtg gacaaagtgg cattcacagg ctccactgag attggccgcg 1561 taatccaggt tgctgctggg agcagcaacc tcaagagagt gaccttggag ctggggggga 1621 agagccccaa catcatcatg tcagatgccg atatggattg ggccgtggaa caggcccact 1681 tcgccctgtt cttcaaccag ggccagtgct gctgtgccgg ctcccggacc ttcgtgcagg 1741 aggacatcta tgatgagttt gtggagcgga gcgttgcccg ggccaagtct cgggtggtcg 1801 ggaacccctt tgatagcaag accgagcagg ggccgcaggt ggatgaaact cagtttaaga 1861 agatcctcgg ctacatcaac acggggaagc aagagggggc gaagctgctg tgtggtgggg 1921 gcattgctgc tgaccgtggt tacttcatcc agcccactgt gtttggagat gtgcaggatg 1981 gcatgaccat cgccaaggag gagatcttcg ggccagtgat gcagatcctg aagttcaaga 2041 ccatagagga ggttgttggg agagccaaca attccacgta cgggctggcc gcagctgtct 2101 tcacaaagga tttggacaag gccaattacc tgtcccaggc cctccaggcg ggcactgtgt 2161 gggtcaactg ctatgatgtg tttggagccc agtcaccctt tggtggctac aagatgtcgg 2221 ggagtggccg ggagttgggc gagtacgggc tgcaggcata cactgaagtg aaaactgtca 2281 cagtcaaagt gcctcagaag aactcaggat ccgactacaa agaccatgac ggtgattata 2341 aagatcatga catcgactac aaggatgacg atgacaagta gtgagggccc gatatctcga 2401 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc 2461 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg 2521 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt 2581 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac 2641 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc 2701 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct 2761 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct 2821 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct 2881 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct 2941 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaaacgg 3001 gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca 3061 ataaaaagac agaataaaac gcacgggtgt tgggtcgttt gttcataaac gcggggttcg 3121 gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg 3181 cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc 3241 caacgtcggg gcggcaggcc ctgccatagc agatctgcgc agctggggct ctagggggta 3301 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt 3361 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct 3421 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg ggcatccctt tagggttccg 3481 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag 3541 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa 3601 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga 3661 tttataaggg attttgggga tttcggccta ttggttaaaa aatgagctga tttaacaaaa 3721 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc 3781 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga 3841 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 3901 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat 3961 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc 4021 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag 4081 ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt 4141 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta 4201 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg 4261 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa 4321 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct 4381 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg 4441 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca 4501 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat 4561 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac 4621 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc 4681 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa 4741 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag 4801 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc 4861 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt 4921 cttgacgagt tcttctgagc gggactctgg ggttcgcgaa atgaccgacc aagcgacgcc 4981 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg 5041 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt 5101 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 5161 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 5221 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc 5281 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg 5341 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat 5401 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 5461 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct 5521 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 5581 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 5641 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 5701 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 5761 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 5821 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 5881 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 5941 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 6001 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 6061 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 6121 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 6181 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 6241 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 6301 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 6361 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 6421 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 6481 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 6541 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 6601 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 6661 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 6721 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 6781 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 6841 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 6901 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 6961 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 7021 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 7081 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 7141 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 7201 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 7261 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 7321 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 7381 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 7441 cgacggatcg ggagatctcc cgatccccta tggtcgactc tcagtacaat ctgctctgat 7501 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc 7561 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg 7621 cttagg //