LOCUS       V020455                11195 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V020455
VERSION     V020455
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             683..5875
                     /label="ARHGEF28(NM_001080479)"
                     /note="ARHGEF28(NM_001080479)"
                     /gene="ARHGEF28"
     CDS             5882..5947
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     misc_feature    5971..6559
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    6625..6673
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6875..7303
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        7317..7646
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             7713..8507
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    8683..8804
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(8853..8869)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8877..8893
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8901..8931)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8946..8967
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9255..9843)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10014..10874)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10875..10979)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccgcca ccatggagtt gagctgcagc gaagcacctc tttacgggca
      721 gatgatgatc tatgcgaagt ttgacaaaaa tgtgtatctt cctgaagatg ctgagtttta
      781 ctttacttat gacggatctc atcagcgaca tgtcatgatt gcagagcgca tcgaggataa
      841 cgttctccag tccagcgtcc caggccatgg gcttcaggag acggtgacgg tatctgtgtg
      901 cctctgctcg gaaggttact ctccggtgac catgggctct ggctcagtga cctacgtgga
      961 caacatggct tgcaggctgg ctcgtctgct ggtgacgcag gccaatcgcc tcacagcctg
     1021 cagccaccag accctgctga ccccatttgc cttgacggca ggagcactgc ctgccttgga
     1081 tgaggagctc gtgctggctc tgacccatct ggaattgcct ctagagtgga ctgtgttggg
     1141 aagttcttca cttgaagtat cttctcacag agaatctctt ctacacctgg ctatgagatg
     1201 gggcctggct aaactttccc agttcttctt gtgtctcccg gggggagtcc aggccttggc
     1261 tttacccaac gaagagggtg ccacaccatt agacttagct ttacgtgaag gacactccaa
     1321 gctggtggaa gacgtcacaa attttcaggg cagatggtcc ccaagcttct cccgagtgca
     1381 gctcagtgaa gaagcctcct tgcattacat tcactcatcg gaaacgctga ccctgaccct
     1441 gaaccacaca gccgagcatt tgttggaggc agatattaaa ctcttccgga aatacttttg
     1501 ggatagagcc tttcttgtca aggcctttga gccagaagcc aggccagagg aaagaacagc
     1561 tatgccctcc agcggtgcag aaactgaaga agagattaag aattcagtgt ccagcagatc
     1621 agcagctgaa aaggaagata taaagcgtgt caaaagcctg gtggttcaac acaatgaaca
     1681 tgaagaccag cacagcctag atttggatcg ctccttcgat atcctaaaaa aatccaagcc
     1741 gccctcgaca ttgcttgctg caggccggct ttcagacatg ctgaatggag gtgatgaagt
     1801 ctacgctaac tgtatggtga ttgatcaggt tggtgatttg gatatcagct atattaatat
     1861 agagggaatc actgccacta ccagccctga atccagaggt tgcactctgt ggcctcagag
     1921 cagcaaacac acccttccta cagaaaccag tcccagtgtg tacccactta gtgaaaatgt
     1981 cgaagggaca gcacacactg aagcccagca gtccttcatg tcaccatcaa gttcgtgtgc
     2041 ttccaacttg aatctttctt ttggttggca tggatttgaa aaggaacaaa gtcatctaaa
     2101 gaaaagaagt tctagccttg atgccttgga cgccgacagt gaaggggaag ggcattctga
     2161 gccatcccac atctgttaca ctccagggtc tcagagctcc tcaagaactg ggattcctag
     2221 tggggatgaa ttggactctt ttgagactaa cactgaaccg gattttaata tctccagggc
     2281 tgaatccctt cctctatcaa gtaatctaca gtcgaaggaa tcactgcttt ctggagttcg
     2341 ctcacgttct tattcttgct catcacccaa aatttcttta ggaaaaactc gtttggtgcg
     2401 tgaattaaca gtatgcagtt caagtgaaga gcaaagagct tacagcttat cggagccacc
     2461 aagagaaaac agaattcagg aagaagaatg ggataaatac atcatacctg ccaaatcaga
     2521 gtctgaaaaa tataaagtga gtcgaacttt cagtttcctc atgaatagga tgactagccc
     2581 tcggaataaa tcaaagacaa aaagcaagga tgccaaagat aaagagaagc tgaatcgaca
     2641 tcagtttgcc ccaggaacat tctctggggt tctgcagtgt ttggtttgtg ataaaacact
     2701 cctggggaaa gagtcactgc agtgttctaa ctgtaatgca aatgtgcaca aaggttgtaa
     2761 agatgctgcg cctgcatgca ccaagaaatt ccaagagaaa tataacaaga acaaaccaca
     2821 gaccatcctt ggaaattctt catttagaga catcccacag cctggtctct ccttgcaccc
     2881 ttcttcctcc gtgcctgttg gattgccgac tggaaggagg gagactgtgg gacaggtcca
     2941 tccattgtcc agaagtgttc caggcaccac cttggaaagc ttcaggaggt cagccacatc
     3001 cttggagtct gagagtgacc ataacagctg cagaagcagg tctcattctg atgagctgct
     3061 acagtccatg ggctcttctc cctctacaga gtctttcata atggaagatg ttgtggattc
     3121 ttctctgtgg agtgacctca gcagtgatgc ccaggagttt gaagcagaat cttggagtct
     3181 tgtggtggat ccctcatttt gtaataggca ggagaaggat gtcatcaaaa gacaggatgt
     3241 catttttgag ctaatgcaaa cagagatgca tcacatccag accctgttca tcatgtctga
     3301 gatcttcagg aaaggcatga aagaggagct gcagctggac cacagcaccg tggataaaat
     3361 tttcccctgt ttagatgagt tgcttgaaat ccacaggcat ttcttctaca gtatgaagga
     3421 acgaaggcag gaatcctgtg ctggcagcga caggaatttt gtgatcgacc gaattggaga
     3481 tattttggta caacagtttt cagaagaaaa tgcaagtaaa atgaagaaaa tatatggaga
     3541 attctgttgc catcataaag aagctgttaa cctctttaaa gaactccagc agaataaaaa
     3601 gtttcagaat tttattaagc tccgaaatag taatcttttg gctcgacgcc gaggaattcc
     3661 agaatgcatt ctgttggtca ctcagcgtat tacaaaatac cctgtcttgg tggaaaggat
     3721 attgcagtac acaaaggaaa gaactgagga acataaagac ttacgcaaag cgctttgctt
     3781 aattaaagac atgattgcaa cagtggattt aaaagtcaat gaatatgaga aaaaccaaaa
     3841 atggcttgag atcctaaata agattgaaaa caaaacatac acgaagctca aaaatggaca
     3901 tgtgtttagg aagcaggcac tgatgagtga agaaaggact ctgttatatg atggccttgt
     3961 ttactggaaa actgctacag gtcgtttcaa agatatccta gctctacttc taactgatgt
     4021 gctgctcttt ttacaagaaa aagaccagaa atacatcttt gcagccgttg atcagaagcc
     4081 atcagttatt tcccttcaaa agcttattgc tagagaagtt gctaatgagg agagaggaat
     4141 gtttctgatc agtgcttcat ctgctggtcc tgagatgtat gaaattcaca ccaattccaa
     4201 ggaggaacgc aataactgga tgagacggat ccagcaggct gtagaaagtt gtcctgaaga
     4261 aaaaggggga aggacaagtg aatctgatga agacaagagg aaagctgaag ccagagtggc
     4321 caaaattcag caatgtcaag aaatactcac taaccaagac caacaaattt gtgcgtattt
     4381 ggaggagaag ctgcatatct atgctgaact tggagaactg agcggatttg aggacgtcca
     4441 tctagagccc cacctcctta ttaaacctga cccaggcgag cctccccagg cagcctcatt
     4501 actggcagca gcactgaaag aagctgagag cctacaagtt gcagtgaagg cctcacagat
     4561 gggcgccgtg agtcaatcat gtgaggacag ttgtggagac tctgtcttgg cggacacact
     4621 cagttctcat gatgtaccag gatcaccgac tgcctcatta gtcacaggag ggagagaagg
     4681 aagaggctgt tcggatgtgg atcccgggat ccagggtgtg gtaaccgact tggccgtctc
     4741 tgatgcaggg gagaaggtgg aatgtagaaa ttttccaggt tcttcacaat cagagattat
     4801 acaagccata cagaatttaa cccgtctctt atacagcctt caggccgcct tgaccattca
     4861 ggacagccac attgagatcc acaggctggt tctccagcag caggagggcc tgtctctcgg
     4921 ccactctatc ctccgaggcg gccccttgca ggaccagaag tctcgcgacg cggacaggca
     4981 gcatgaggag ctggccaatg tgcaccagct tcagcaccag ctccagcagg agcagcggcg
     5041 ctggctgcgc aggtgtgagc agcagcagcg ggcgcaggcg accagggaga gctggctgca
     5101 ggagcgggag cgggagtgcc agtcgcagga ggagctgctg ctgcggagcc ggggcgagct
     5161 ggacctccag ctccaggagt accagcacag cctggagcgg ctgagggagg gccagcgcct
     5221 ggtggagagg gagcaggcga ggatgcgggc ccagcagagc ctgctgggcc actggaagca
     5281 cggccggcag aggagcctgc ccgcggtgct ccttccgggt ggccccgagg taatggaact
     5341 taatcgatct gagagtttat gtcatgaaaa ctcattcttc atcaatgaag ctttagtaca
     5401 aatgtcattt aacactttca acaaactgaa tccatcagtt atccatcagg atgccactta
     5461 ccctacaact caatctcatt ctgacttggt gaggactagt gaacatcaag tagacctcaa
     5521 ggtggaccct tctcagcctt cgaatgtcag tcacaaactg tggacagccg ctggttccgg
     5581 ccatcagata cttcctttcc atgaaagcag caaggattct tgtaaaaatg gctccagtat
     5641 gacaaagtgc agttgtacgt tgacatctcc cccgggactg tggactggaa ccacatctac
     5701 tttgaaagat ttggacacct cccacactga gtccccaacc ccccatgact caaattcaca
     5761 ccgccctcaa ctgcaggcgt ttataacaga agcaaagcta aatctaccga caaggacaat
     5821 gaccagacaa gatggggaaa ctggagatgg agccaaagaa aatattgttt acctcctcga
     5881 ggactacaaa gaccatgacg gtgattataa agatcatgac atcgactaca aggatgacga
     5941 tgacaagtag tgagggcccg atatctcgac aatcaacctc tggattacaa aatttgtgaa
     6001 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     6061 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     6121 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     6181 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     6241 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     6301 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     6361 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     6421 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     6481 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     6541 ctttgggccg cctccccgcc tggaaacggg ggaggctaac tgaaacacgg aaggagacaa
     6601 taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacgggtgtt
     6661 gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca
     6721 ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca
     6781 agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagca
     6841 gatctgcgca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     6901 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     6961 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     7021 ctaaatcggg gcatcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     7081 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     7141 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     7201 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttggggat ttcggcctat
     7261 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
     7321 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     7381 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     7441 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     7501 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     7561 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
     7621 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
     7681 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     7741 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     7801 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     7861 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     7921 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     7981 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     8041 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     8101 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     8161 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     8221 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
     8281 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     8341 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     8401 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     8461 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     8521 gttcgcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
     8581 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     8641 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     8701 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     8761 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     8821 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     8881 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     8941 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     9001 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     9061 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     9121 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     9181 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     9241 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     9301 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     9361 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     9421 ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg
     9481 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     9541 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     9601 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     9661 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     9721 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     9781 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     9841 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     9901 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     9961 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
    10021 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
    10081 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
    10141 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
    10201 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
    10261 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
    10321 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
    10381 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
    10441 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
    10501 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    10561 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    10621 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    10681 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    10741 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    10801 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    10861 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    10921 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    10981 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat
    11041 ggtcgactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
    11101 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
    11161 gcttgaccga caattgcatg aagaatctgc ttagg
//
