LOCUS V040640 8028 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V040640 VERSION V040640 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 706..2754 /label="Acsl5(NM_027976)" /note="Acsl5(NM_027976)" /gene="Acsl5" CDS 2761..2784 /codon_start=1 /product="8xHis affinity tag" /transl_table=1 /translation="HHHHHHHH" /label="8xHis" misc_feature 2804..3392 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 3458..3506 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 3708..4136 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4150..4479 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4546..5340 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5516..5637 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5686..5702) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5710..5726 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5734..5764) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5779..5800 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6088..6676) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6847..7707) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7708..7812) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg 661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgct ttttattttt 721 aacttcttgt tttccccact tccaactccg gccctgatct gcctcctgac gtttggaacg 781 gccatcttcc tgtggctgat caacagacct cagccagttt tacctctcat tgacttggac 841 aaccagtctg tggggattga gggaggagca cggaggggcg ctttccagaa gaacaatgac 901 ctaatccttt attacttctc agacgccaag acgttgtatg aaaatttcca aagaggactt 961 gctgtgtctg acaatgggcc ttgcttggga tacagaaagc caaaccagcc ctacaaatgg 1021 atctcctaca aacaggtgtc tgatcgagca gagtacctgg gctcctgtct tttgcataaa 1081 ggatataagt catcacagga ccaatttgtt ggcatctttg ctcaaaatag gccagagtgg 1141 gtcatctctg agttagcctg ttacacatat tccatggtag ctgtccccct gtatgacacg 1201 ctgggtacag aagccatcat ctttgtcatc aacagggctg atatccctgt ggtcatctgt 1261 gatacacccc aaaaggcaac aatgctagta gaaaatgtgg aaaagggcct cacaccaggc 1321 ctgaagacga tcatcctcat ggaccctttt gatgacgacc tgatgaaaag aggagaaaag 1381 tgtggagttg agatgttatc cctgcatgat gcggagaata taggcaaaga gaacttcaaa 1441 aaaccagtgc ctccgaagcc agaggatctg agcgtcatct gtttcaccag tggaactaca 1501 ggtgacccca aaggagctat gctaacccat gaaaatgttg tttcaaacat ggctgctttc 1561 ctcaaatttc tggagcctat cttccagccc acctctgatg atgtgaccat atcctacctt 1621 cccttggctc atatgtttga gaggcttgtc cagggtatct tattttcctg tggaggcaaa 1681 attgggttct tccaaggaga tatccggttg ctacctgatg acatgaaggc tttgaaaccc 1741 acagtgtttc ctacagtgcc tcgactcctt aacagggtct atgataaggt acaaaatgaa 1801 gccaagacac ctttgaagaa gttcttgttg aacttggcta tcatcagtaa attcaatgaa 1861 gtgaaaaatg gcatcattcg gcgggacagt ttgtgggaca agctcgtgtt ttcaaagatt 1921 caaggaagcc tgggggggaa ggttcgcctc atgatcactg gagccgcccc catctccact 1981 ccagtcttga cattcttcag ggctgcaatg ggatgttggg tgtttgaagc ttatggccaa 2041 acagaatgca caggtggatg ttcaattaca tcacctgggg actggacagc aggtcatgtt 2101 gggactccag tggcttgcaa ttttgtaaag ctggaagatg tggctgacat gaactacttt 2161 tcagtaaaca acgaaggcga gatctgcatc aaaggtaaca atgtgttcaa aggctaccta 2221 aaggacccag agaaaacaca ggaagtactg gacaaggatg gatggcttca cactggggac 2281 attggtcgct ggcttccaaa tggaactctg aaaattgttg accggaagaa gaatattttc 2341 aagttggcac aaggagaata cattgcccca gagaagattg aaaatgttta ttccaggagc 2401 agaccagtat tgcaagtttt tgtccatggg gagagcttac ggtcattcct gatcggagtg 2461 gtggttcccg acccagattc acttccctca tttgcagcca agatcggggt aaagggatca 2521 tttgaagaac tgtgcaaaaa ccaatgtgta aaggaagcca ttttagaaga cttgcagaaa 2581 attgggaaag agggtggcct gaaatccttt gagcaggtca aaagtatctt tgtgcatcca 2641 gagcccttca ctattgaaaa cggacttctg acaccgacac tgaaagccaa acgagtagag 2701 cttgccaagt tcttccagac acaaatcaag agcctctatg agagcatcga ggagctcgag 2761 caccatcacc atcaccatca ccattagggc ccgatatctc gacaatcaac ctctggatta 2821 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg 2881 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc 2941 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca 3001 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac 3061 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact 3121 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc 3181 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg 3241 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc 3301 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac 3361 gagtcggatc tccctttggg ccgcctcccc gcctggaaac gggggaggct aactgaaaca 3421 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa 3481 acgcacgggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc 3541 tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc 3601 accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg 3661 ccctgccata gcagatctgc gcagctgggg ctctaggggg tatccccacg cgccctgtag 3721 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag 3781 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt 3841 tccccgtcaa gctctaaatc ggggcatccc tttagggttc cgatttagtg ctttacggca 3901 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata 3961 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca 4021 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttggg 4081 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt 4141 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt 4201 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca 4261 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta 4321 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 4381 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag 4441 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata 4501 tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat 4561 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca 4621 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg 4681 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg 4741 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact 4801 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct 4861 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg 4921 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt 4981 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc 5041 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc 5101 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga 5161 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc 5221 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt 5281 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga 5341 gcgggactct ggggttcgcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga 5401 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc 5461 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt 5521 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 5581 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 5641 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc 5701 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 5761 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 5821 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 5881 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 5941 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 6001 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 6061 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 6121 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 6181 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 6241 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta 6301 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 6361 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 6421 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 6481 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 6541 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 6601 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 6661 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 6721 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 6781 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 6841 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 6901 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 6961 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 7021 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 7081 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 7141 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 7201 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 7261 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 7321 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 7381 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 7441 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 7501 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 7561 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 7621 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 7681 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 7741 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 7801 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct 7861 cccgatcccc tatggtcgac tctcagtaca atctgctctg atgccgcata gttaagccag 7921 tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct 7981 acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagg //