LOCUS       V031190                 6222 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031190
VERSION     V031190
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2369
                     /label="Agmo(NM_178767)"
                     /note="Agmo(NM_178767)"
                     /gene="Agmo"
     CDS             2388..2417
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2436..2459
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2479..2495)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2506..3128
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3277..3865)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4194..4241
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4473..5267)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5302..5659)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5661..5765)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5792..6220
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gaggagccca ggagcccaag acaatgtttc agtttctcag ggtatgcgag
     1081 cgatgtttta catgatggag cccagtgaaa ctgccttcca aactgttgaa gaggtgcctg
     1141 attacgtaaa aaaggcgact ccatttttta tcttcttgat tctgcttgaa ctagtcatca
     1201 gctggattct caaagggaaa ccatcaggtc gtttggatga tgctctcaca tctatatcag
     1261 ctggtgttgt gtctcggctt ccaagtctgt tcttcaggag ccttgaagtg accagttata
     1321 tttatatctg ggaaaactac agacttttgg aattgccttg ggattctacc tggacgtggt
     1381 atttcacttt cttaggagtt gactttggct actactggtt ccaccgcatg gctcatgaga
     1441 ttaatatttt ctgggcagca caccaagcac accacagttc tgaagactat aacttatcca
     1501 cagcactgag acagtctgtc ttgcagcagt atagttcctg ggttttctac tgtccactgg
     1561 cccttttcat accaccttca gtgtttgctg ttcatattca attcaatctc ctctaccaat
     1621 tttggatcca cacagagatc atcagaaccc ttggcccttt ggaagtgatc cttaatactc
     1681 ctagccatca cagagttcat catggtagaa atcgttactg cattgataaa aactatgctg
     1741 gcactcttat tatttgggat agaatttttg ggacatttga agcagagaat gagcaggtca
     1801 tatatggtct aacccatccc attggtacat ttgagccatt caacgtgcag tttcaccatt
     1861 tactttatat atggactaca ttctggacca cacctggatt ctgccataag ttttctgtcc
     1921 tatttaaagg accaggatgg ggtccaggta aaccaagact tggcctgagt gaagaaattc
     1981 cagaggttac aggacaggaa gtccccttct catcctcagc atcccagctt ttaaagatct
     2041 acactgtcct ccagtttgca gtaatgctgg cattttatga agagaccttt gcaaacacag
     2101 cggtactgtc ccaggtcact cttcttctga gaatcttctt ctttatcctg accttgacgt
     2161 ccattggttt tcttctggac caaagatcca aggcagctac tatggaaact ttccggtgcc
     2221 tgttgttcct aaccctgcac agatttggtc acttaaagcc tcttattcca tctttatctt
     2281 ttgcatttga gattttcttt tcggtttgca ttgctttctg gggcgtaaga agcataacac
     2341 aactcacctc tggctcttgg aaaaagccta cgcgtacgcg gccgctcgag cagaaactca
     2401 tctcagaaga ggatctggca gcaaatgata tcctggatta caaggatgac gacgataagg
     2461 tttaaacggc cggccgcggt catagctgtt tcctgaacag atcccgggtg gcatccctgt
     2521 gacccctccc cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt
     2581 gtcctaataa aattaagttg catcattttg tctgactagg tgtccttcta taatattatg
     2641 gggtggaggg gggtggtatg gagcaagggg caagttggga agacaacctg tagggcctgc
     2701 ggggtctatt gggaaccaag ctggagtgca gtggcacaat cttggctcac tgcaatctcc
     2761 gcctcctggg ttcaagcgat tctcctgcct cagcctcccg agttgttggg attccaggca
     2821 tgcatgacca ggctcagcta atttttgttt ttttggtaga gacggggttt caccatattg
     2881 gccaggctgg tctccaactc ctaatctcag gtgatctacc caccttggcc tcccaaattg
     2941 ctgggattac aggcgtgaac cactgctccc ttccctgtcc ttctgatttt aaaataacta
     3001 taccagcagg aggacgtcca gacacagcat aggctacctg gccatgccca accggtggga
     3061 catttgagtt gcttgcttgg cactgtcctc tcatgcgttg ggtccactca gtagatgcct
     3121 gttgaattgg gtacgcggcc agcggcgagc ggtatcagct cactcaaagg cggtaatacg
     3181 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     3241 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     3301 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     3361 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     3421 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     3481 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     3541 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     3601 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     3661 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     3721 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     3781 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     3841 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     3901 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     3961 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     4021 acctgaggct atggcagggc ctgccgcccc gacgttggct gcgagccctg ggccttcacc
     4081 cgaacttggg gggtggggtg gggaaaagga agaaacgcgg gcgtattggc cccaatgggg
     4141 tctcggtggg gtatcgacag agtgccagcc ctgggaccga accccgcgtt tatgaacaaa
     4201 cgacccaaca ccgtgcgttt tattctgtct ttttattgcc gtcatagcgc gggttccttc
     4261 cggtattgtc tccttccgtg tttcagttag cctcccccta gggtgggcga agaactccag
     4321 catgagatcc ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc
     4381 caacctttca tagaaggcgg cggtggaatc gaaatctcgt gatggcaggt tgggcgtcgc
     4441 ttggtcggtc atttcgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag
     4501 aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc
     4561 cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcga
     4621 tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg
     4681 atattcggca agcaggcatc gccatgggtc acgacgagat cctcgccgtc gggcatgctc
     4741 gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca
     4801 tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct
     4861 tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc
     4921 atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact
     4981 tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa
     5041 ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcttgcag ttcattcagg
     5101 gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac
     5161 acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc
     5221 acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct
     5281 catcctgtct cttgatcgat ctttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta
     5341 cttctggaat agctcagagg ccgaggcggc ctcggcctct gcataaataa aaaaaattag
     5401 tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt
     5461 taggggcggg actatggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc
     5521 tggggagcct ggggactttc cacacctggt tgctgactaa ttgagatgca tgctttgcat
     5581 acttctgcct gctggggagc ctggggactt tccacaccct aactgacaca cattccacag
     5641 ctggttcttt ccgcctcagg actcttcctt tttcaatatt attgaagcat ttatcagggt
     5701 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     5761 ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg
     5821 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     5881 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     5941 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     6001 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     6061 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     6121 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     6181 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt tt
//
