LOCUS       V031214                 5475 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031214
VERSION     V031214
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..1622
                     /label="Aicda(NM_009645)"
                     /note="Aicda(NM_009645)"
                     /gene="Aicda"
     CDS             1641..1670
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             1689..1712
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(1732..1748)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    1759..2381
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(2530..3118)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    3447..3494
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(3726..4520)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(4555..4912)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(4914..5018)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5045..5473
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggacagcctt ctgatgaagc aaaagaagtt tctttaccat ttcaaaaatg
     1081 tccgctgggc caagggacgg catgagacct acctctgcta cgtggtgaag aggagagata
     1141 gtgccacctc ctgctcactg gacttcggcc accttcgcaa caagtctggc tgccacgtgg
     1201 aattgttgtt cctacgctac atctcagact gggacctgga cccgggccgg tgttaccgcg
     1261 tcacctggtt cacctcctgg agcccgtgct atgactgtgc ccggcacgtg gctgagtttc
     1321 tgagatggaa ccctaacctc agcctgagga ttttcaccgc gcgcctctac ttctgtgaag
     1381 accgcaaggc tgagcctgag gggctgcgga gactgcaccg cgctggggtc cagatcggga
     1441 tcatgacctt caaagactat ttttactgct ggaatacatt tgtagaaaat cgtgaaagaa
     1501 ctttcaaagc ctgggaaggg ctacatgaaa attctgtccg gctaaccaga caacttcggc
     1561 gcatcctttt gcccttgtac gaagtcgatg acttgcgaga tgcatttcgt atgttgggat
     1621 ttacgcgtac gcggccgctc gagcagaaac tcatctcaga agaggatctg gcagcaaatg
     1681 atatcctgga ttacaaggat gacgacgata aggtttaaac ggccggccgc ggtcatagct
     1741 gtttcctgaa cagatcccgg gtggcatccc tgtgacccct ccccagtgcc tctcctggcc
     1801 ctggaagttg ccactccagt gcccaccagc cttgtcctaa taaaattaag ttgcatcatt
     1861 ttgtctgact aggtgtcctt ctataatatt atggggtgga ggggggtggt atggagcaag
     1921 gggcaagttg ggaagacaac ctgtagggcc tgcggggtct attgggaacc aagctggagt
     1981 gcagtggcac aatcttggct cactgcaatc tccgcctcct gggttcaagc gattctcctg
     2041 cctcagcctc ccgagttgtt gggattccag gcatgcatga ccaggctcag ctaatttttg
     2101 tttttttggt agagacgggg tttcaccata ttggccaggc tggtctccaa ctcctaatct
     2161 caggtgatct acccaccttg gcctcccaaa ttgctgggat tacaggcgtg aaccactgct
     2221 cccttccctg tccttctgat tttaaaataa ctataccagc aggaggacgt ccagacacag
     2281 cataggctac ctggccatgc ccaaccggtg ggacatttga gttgcttgct tggcactgtc
     2341 ctctcatgcg ttgggtccac tcagtagatg cctgttgaat tgggtacgcg gccagcggcg
     2401 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     2461 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     2521 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     2581 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     2641 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     2701 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     2761 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     2821 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     2881 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     2941 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     3001 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     3061 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     3121 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     3181 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     3241 aagttttaaa tcaatctaaa gtatatatga gtaacctgag gctatggcag ggcctgccgc
     3301 cccgacgttg gctgcgagcc ctgggccttc acccgaactt ggggggtggg gtggggaaaa
     3361 ggaagaaacg cgggcgtatt ggccccaatg gggtctcggt ggggtatcga cagagtgcca
     3421 gccctgggac cgaaccccgc gtttatgaac aaacgaccca acaccgtgcg ttttattctg
     3481 tctttttatt gccgtcatag cgcgggttcc ttccggtatt gtctccttcc gtgtttcagt
     3541 tagcctcccc ctagggtggg cgaagaactc cagcatgaga tccccgcgct ggaggatcat
     3601 ccagccggcg tcccggaaaa cgattccgaa gcccaacctt tcatagaagg cggcggtgga
     3661 atcgaaatct cgtgatggca ggttgggcgt cgcttggtcg gtcatttcga accccagagt
     3721 cccgctcaga agaactcgtc aagaaggcga tagaaggcga tgcgctgcga atcgggagcg
     3781 gcgataccgt aaagcacgag gaagcggtca gcccattcgc cgccaagctc ttcagcaata
     3841 tcacgggtag ccaacgctat gtcctgatag cgatccgcca cacccagccg gccacagtcg
     3901 atgaatccag aaaagcggcc attttccacc atgatattcg gcaagcaggc atcgccatgg
     3961 gtcacgacga gatcctcgcc gtcgggcatg ctcgccttga gcctggcgaa cagttcggct
     4021 ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat cgacaagacc ggcttccatc
     4081 cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca ggtagccgga
     4141 tcaagcgtat gcagccgccg cattgcatca gccatgatgg atactttctc ggcaggagca
     4201 aggtgagatg acaggagatc ctgccccggc acttcgccca atagcagcca gtcccttccc
     4261 gcttcagtga caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc cagccacgat
     4321 agccgcgctg cctcgtcttg cagttcattc agggcaccgg acaggtcggt cttgacaaaa
     4381 agaaccgggc gcccctgcgc tgacagccgg aacacggcgg catcagagca gccgattgtc
     4441 tgttgtgccc agtcatagcc gaatagcctc tccacccaag cggccggaga acctgcgtgc
     4501 aatccatctt gttcaatcat gcgaaacgat cctcatcctg tctcttgatc gatctttgca
     4561 aaagcctagg cctccaaaaa agcctcctca ctacttctgg aatagctcag aggccgaggc
     4621 ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tggggcggag aatgggcgga
     4681 actgggcgga gttaggggcg ggatgggcgg agttaggggc gggactatgg ttgctgacta
     4741 attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacacct
     4801 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga
     4861 ctttccacac cctaactgac acacattcca cagctggttc tttccgcctc aggactcttc
     4921 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     4981 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     5041 cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     5101 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     5161 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     5221 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     5281 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     5341 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     5401 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     5461 tttaacgcga atttt
//
