LOCUS       V030162                11148 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030162
VERSION     V030162
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..7295
                     /label="Arhgap32(NM_001195632)"
                     /note="Arhgap32(NM_001195632)"
                     /gene="Arhgap32"
     CDS             7314..7343
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             7362..7385
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(7405..7421)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    7432..8054
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(8203..8791)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    9120..9167
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(9399..10193)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(10228..10585)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(10587..10691)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      10718..11146
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggagacagaa agtgagacta gctctttggg ggatgacagt gtgttttggt
     1081 tggactgtga aggtgtaacc cagctgactg atggtgacga ggaagaaagg gaagagagtt
     1141 tcagaaagat gaagtcctca atacattctg aagaggatga ttttgttcca gagctacata
     1201 gaaatgttca cccccgagag cggcctgatt gggaggaaac actcagcgca atggcaagag
     1261 gagcagatgt ccctgagatc cctggagacc tgactcttaa gtcctgtggc agtacagcga
     1321 gcacgaaggt taagcatgtg aagaagttac ccttcacaaa gggacacttt ccaaagatgg
     1381 ctgaatgtgc acattttcat tatgagaacg tggagtttgg cagcatacag ctctcactat
     1441 cagaagaaca gaatgaagta atgaaaaatg gctgtgaatc taaagagctg gtctacctcg
     1501 tgcagattgc ctgtcagggc aagagttgga ttgttaaaag aagttatgaa gattttcggg
     1561 tactcgacaa acatcttcat ctctgcattt atgaccgccg attctcccag ctcacagagc
     1621 ttccccgctc tgacgtcctg aaggacagtc ctgagtcggt tactcagatg cttacagctt
     1681 acctgtcacg cctttcaact atcgctggca acaagatcaa ctgtgggcct gcccttacct
     1741 ggatggagat cgacaataag ggaaaccatc ttctagttca cgaggagtcg tccatcaaca
     1801 ctcctgctgt cggtgctgcc catgttatca agcgctacac cgctcgggcc cctgatgaac
     1861 tgacattaga ggtgggagac atcgtttctg ttattgacat gcctcccaaa gtattaagca
     1921 catggtggag aggcaagcat ggatttcagg tgggactctt ccctggtcat tgtgtcgagt
     1981 taattaacca gaaagttcct cagtcagtaa ccaactcagt gccaaaacca gtgtctaaaa
     2041 agcacggcaa gctcattact ttcttgcgaa cattcatgaa gtctcgccca acaaaacaga
     2101 agctaaagca gcggggcatc ttgaaagaga gagtattcgg ttgtgacttg ggggagcacc
     2161 tcctcaattc tgggtttgaa gtgccccagg ttcttcagag ctgcacagcg ttcatcgaga
     2221 ggtatggcat tgtggatggg atatatcgtc tctctggcgt tgcctccaat atccagagac
     2281 ttcgtcatga atttgattct gagcatgtcc ccgacctgac aaaagaacct tatgttcaag
     2341 acatccattc tgtgggctcc ctctgtaagc tgtacttccg agaactccca aaccctctgc
     2401 tcacctacca gctatacgag aagttctctg atgctgtttc agcagcaacg gatgaagaac
     2461 ggttgataaa aatccatgat gtcatccagc aactcccccc accacactat agaaccctgg
     2521 agttcctgat gagacacctg tctcttctag cagactattg ctccatcaca aatatgcatg
     2581 cgaaaaacct tgccattgtg tgggcaccaa acctgctgag atcaaagcag atagagtcag
     2641 cttgcttcag tgggacagca gctttcatgg aagtgaggat tcagtctgtg gttgttgaat
     2701 tcattctcaa tcatgtggat gtgctcttca gtggcaaaat cagcgctgtc atgcaggaag
     2761 gagcagcttc tctgtcaagg cccaagtccc tgctggtctc gtctccatct accaagctgc
     2821 tgacattgga ggaagcccag gcaaggacgc aggctcaggt cagttctcct attgtgacgg
     2881 aaaataagta catcgaagtc ggagaagggc ctgctgcgct gcaagggaag tttcacaccg
     2941 tcattgagtt cccacttgaa agaaagaggc ctcagaataa gatgaaaaaa tctcccgttg
     3001 gcagctggcg ttcctttttc aatttgggga agtcctcttc tgtctccaaa cgcaagttgc
     3061 agcgtaatga aagtgagccc tcagaaatga aagccatggc tttgaaaggt ggcagggcag
     3121 aaggaaccct ccgctcagct aaaagtgaag agtcccttac ttctctccat gcagttgacg
     3181 gtgattccaa gttgttccga cctagaagac ccagatccag cagcgatgcg ctctctgcct
     3241 ctttcaatgg cgacgtgctg gggaaccggt gcaattccta tgacaatctg ccccacgaca
     3301 acgagagtga agaggaagtg gggctgctcc acattccagc tcttgtgtct cctcattcgg
     3361 ctgaggatgt tgacttgagt ccaccagaca tcggagtggc cagtctggac tttgatccca
     3421 tgtcatttca atgtagtcct ccaaaagctg agtcagagtg tctggagagt ggtgcttcct
     3481 ttctggattc attaggttat accagggata aactgagtcc cagtaagaag gatgcagaag
     3541 caggtggtag ccagtcacag actccaggaa gcaccgcaag ttccgaacct gtatctccag
     3601 tccaggagaa actgagtccg tttttcaccc tggacttgag ccccactgac gacaagtctt
     3661 ctaagccatc ttcatttaca gaaaaggttg tttatgcttt ctctccgaag attgggcgga
     3721 agctaagcaa atctccttcc atgaacatat ctgagcctat ttcagtgact ctcccacctc
     3781 gggtatcaga agtcatcggt acggtctcca atactgtagc tcagaatgca tcacctacat
     3841 cttgggacaa aagtgtggaa gaaagagatg tcataaatag atcccccacc cagctgcagc
     3901 taggaaagat gaaagcaggt gagagagagg cacaggagac ctgtgaacca gaagcacagc
     3961 ccctggaaca gggggcagct gaagaagtag agttgccagg gacagaggag cggcctgtcc
     4021 tgagcagtca gagcaaggct gtaccgtctg gacagagtca gacaggagca gttacccatg
     4081 acccccctca ggatcccgtt cctgtcagtt cagtctctct tatcccacca ccaccgcctc
     4141 cgaaaaatgt cgcccgaatg ttggcactag cgttagctga gtctgcacag caagcctcaa
     4201 gtcagacact gaagagacca ggggcttccc aggctgggtg cactagttat ggagacacgg
     4261 cagtcgttcc atctgaagag aagctgccta gttcctactc tagccttact ctagataaaa
     4321 cctgtttcca aacggaccgg ccagcagagc agttccaccc gcagatcaat ggactcggaa
     4381 actgtaacca gcctctccct gaggcagcag ctatgggagg tcccacccag tccaacacaa
     4441 ctgactctgg ggagcaactc caccaagtag acttaatagg gaattcgcta catcgaaacc
     4501 atatatctgg ggatccagaa aaggctagaa gcacttcagc tcccttaaca gactcagaga
     4561 agtctgatga tcatggaagt ttccctgaag accacgctgg gaagagcagc gtgtccactg
     4621 tctccttttt ggagcaggac cagtctccac tccatttctc ctgtggagat cagcccctct
     4681 cttaccttgg taccagtgtg gataaacccc accattcctc agaacttaca gacaaatctc
     4741 ccatgccttc tactttgcct agggacaaag cccaccaccc tctctctggg tcccctgaag
     4801 agaattccag cacagccacc atggcttaca tgatggcaac tccagcaaga gcggaaccga
     4861 gcaacagcga ggccagcagg gtcctggctg agcagccctc ggctgctgat tttgtggctg
     4921 ccacccttca gcgcacgcac agaactaatc ggcccctccc tccacctcct tcccagaggc
     4981 ctgcagagca gccaccagtt gtggggcagg tacaagaagc accaagtata ggattaaata
     5041 attcccacaa ggtccaggga acggctccag ctcccgagag gccacctgag tctcgggcca
     5101 tgggtgaccc cgcacccatc ttcctcagtg atggcactgc tgctgcccag tgtcccatgg
     5161 gcgcctctgc tcctcagcct ggactgcctg agaaggttcg ggaaagcagc agggctccac
     5221 cactccactt acgtgctgag tcttttcctg gccattcctg tggctttgct gccccagttc
     5281 ccccaacacg gacaatggag agcaagatgg ctgctgcctt gcactccagc gctgcagatg
     5341 ccaccagcag ttcaaattac cactcctttg tcccttcgtc agcctcagtg gatgatgtga
     5401 tgcccgtgcc actccccgtc tcacagccta agcatgcttc tcagaagata gcctattcct
     5461 cctttgctag gcctgatgtc accgcagagc cctttggtcc agaaaactgt ttgcatttca
     5521 atatgactcc aaactgccag tttcgccccc agagtgttcc tccacaccac aataagttgg
     5581 agccacatca ggtatatggt gcccggtcag agccaccagc ctccatgggt cctcgttata
     5641 acacctatgt ggcgccggga agaaacatgt ctggacacca ctccaagccg tgtagtcggg
     5701 ttgagtatgt ctcttctctg ggctcctcgg ttaggaatcc ttgttgccct gaagacattc
     5761 taccttaccc taccatccgg agggtacagt ccctccatgc acccccacct tccatgatcc
     5821 gctctgttcc catttcacgg acagaagttc ccccagatga tgaaccagcc tactgcccaa
     5881 gaccagtcta ccagtataag ccataccagt cctcccaggc ccgctcagac tatcacgtaa
     5941 ctcagcttca gccttacttt gagaatggac gggtccatta tcgctatagt ccgtactcca
     6001 gctcctccag ctcctattac agtcctgagg gggccttgtg tgatgtcgat gcctatggca
     6061 cagtccagct gcggcccctc caccgcctgt ccagtcggga ctttgctttc tacaatccaa
     6121 ggctacaagg aaagaacgtg tacaactatg ctggtttgcc tccacgccca cgggccaatg
     6181 caactggcta tttctctggt aatgaccata atgtagtcac tatgcccccc actgctgacg
     6241 gaaagcacac ctacacctca tgggaccttg aggacatgga aaaataccgc atgcagtcca
     6301 tccgccgaga gagccgagca cggcagaagg taaaagggcc tatcatgtcg cagtatgaca
     6361 acatgacacc agctgtgcaa gaagacttgg gagggatcta tgtcatccat ctgcgcagca
     6421 aatcagatcc tgggaaaact ggacttctct ctgtggcaga ggggaaagag ggacggcacc
     6481 cagccaaggc tgtcagtcct gagggggacg agcgtttcta caggaagcac ccagagtccg
     6541 aatttgacag agcccaccat catggagggt atggcagcac acaggcagaa aagccatccc
     6601 tcccgcagaa gcaaagcagc cttaggaaca gaaagcttca cgatatgggt tgtagcctcc
     6661 cagagcacag ggcgcatcag gaagcaagcc ataggcaatt atgtgaatca aaaaatggac
     6721 caccttatcc ccagggagct ggccagttag attatgggtc caaagggatg ccagacactt
     6781 ctgagccaag caactaccat aactctggga aatatatgac atcagggcag gggtctctaa
     6841 ccctgaacca caaggaagtg aggcttccca aagatctgga cagacctcga gccaggcagc
     6901 cacctggccc tgagaaacac tccagagact gctacaagga ggaggaacac ttctctcagt
     6961 ctatggtccc accccccaaa ccagagagga gtcacagcct aaaacttcac catacccaga
     7021 acctggagag ggaccccagc gtgctatacc agtatcagac acacagcaag cgccagagca
     7081 gcatgactgt tgtgtcccag tatgataacc tggaggatta ccactccctg ccccagcacc
     7141 agcgaggagg ctttggaggg gcgggcatgg gggcctatgt gccctctggc tttgtccatc
     7201 cacagagcag gacatacgcc acagcattgg gtcagggggc cttcctgccc acagagttgt
     7261 ccttgccaca tcccgacacg cagatccatg cagaaacgcg tacgcggccg ctcgagcaga
     7321 aactcatctc agaagaggat ctggcagcaa atgatatcct ggattacaag gatgacgacg
     7381 ataaggttta aacggccggc cgcggtcata gctgtttcct gaacagatcc cgggtggcat
     7441 ccctgtgacc cctccccagt gcctctcctg gccctggaag ttgccactcc agtgcccacc
     7501 agccttgtcc taataaaatt aagttgcatc attttgtctg actaggtgtc cttctataat
     7561 attatggggt ggaggggggt ggtatggagc aaggggcaag ttgggaagac aacctgtagg
     7621 gcctgcgggg tctattggga accaagctgg agtgcagtgg cacaatcttg gctcactgca
     7681 atctccgcct cctgggttca agcgattctc ctgcctcagc ctcccgagtt gttgggattc
     7741 caggcatgca tgaccaggct cagctaattt ttgttttttt ggtagagacg gggtttcacc
     7801 atattggcca ggctggtctc caactcctaa tctcaggtga tctacccacc ttggcctccc
     7861 aaattgctgg gattacaggc gtgaaccact gctcccttcc ctgtccttct gattttaaaa
     7921 taactatacc agcaggagga cgtccagaca cagcataggc tacctggcca tgcccaaccg
     7981 gtgggacatt tgagttgctt gcttggcact gtcctctcat gcgttgggtc cactcagtag
     8041 atgcctgttg aattgggtac gcggccagcg gcgagcggta tcagctcact caaaggcggt
     8101 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     8161 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     8221 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     8281 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     8341 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     8401 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     8461 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     8521 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     8581 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     8641 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     8701 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     8761 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     8821 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     8881 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     8941 tgagtaacct gaggctatgg cagggcctgc cgccccgacg ttggctgcga gccctgggcc
     9001 ttcacccgaa cttggggggt ggggtgggga aaaggaagaa acgcgggcgt attggcccca
     9061 atggggtctc ggtggggtat cgacagagtg ccagccctgg gaccgaaccc cgcgtttatg
     9121 aacaaacgac ccaacaccgt gcgttttatt ctgtcttttt attgccgtca tagcgcgggt
     9181 tccttccggt attgtctcct tccgtgtttc agttagcctc cccctagggt gggcgaagaa
     9241 ctccagcatg agatccccgc gctggaggat catccagccg gcgtcccgga aaacgattcc
     9301 gaagcccaac ctttcataga aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg
     9361 cgtcgcttgg tcggtcattt cgaaccccag agtcccgctc agaagaactc gtcaagaagg
     9421 cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg
     9481 tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga
     9541 tagcgatccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc
     9601 accatgatat tcggcaagca ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc
     9661 atgctcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc
     9721 agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt
     9781 ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca
     9841 tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc
     9901 ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct
     9961 gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ttgcagttca
    10021 ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc
    10081 cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc
    10141 ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcgaaac
    10201 gatcctcatc ctgtctcttg atcgatcttt gcaaaagcct aggcctccaa aaaagcctcc
    10261 tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa
    10321 aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg
    10381 cggagttagg ggcgggacta tggttgctga ctaattgaga tgcatgcttt gcatacttct
    10441 gcctgctggg gagcctgggg actttccaca cctggttgct gactaattga gatgcatgct
    10501 ttgcatactt ctgcctgctg gggagcctgg ggactttcca caccctaact gacacacatt
    10561 ccacagctgg ttctttccgc ctcaggactc ttcctttttc aatattattg aagcatttat
    10621 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    10681 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta
    10741 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
    10801 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
    10861 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
    10921 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
    10981 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
    11041 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
    11101 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaatttt
//
