LOCUS       V019963                 8213 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V019963
VERSION     V019963
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             683..2869
                     /label="BAP1(NM_004656)"
                     /note="BAP1(NM_004656)"
                     /gene="BAP1"
     CDS             2876..2965
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     misc_feature    2989..3577
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    3643..3691
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      3893..4321
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4335..4664
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4731..5525
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5701..5822
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5871..5887)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5895..5911
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5919..5949)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5964..5985
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6273..6861)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7032..7892)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7893..7997)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccgcca ccatgaataa gggctggctg gagctggaga gcgacccagg
      721 cctcttcacc ctgctcgtgg aagatttcgg tgtcaagggg gtgcaagtgg aggagatcta
      781 cgaccttcag agcaaatgtc agggccctgt atatggattt atcttcctgt tcaaatggat
      841 cgaagagcgc cggtcccggc gaaaggtctc taccttggtg gatgatacgt ccgtgattga
      901 tgatgatatt gtgaataaca tgttctttgc ccaccagctg atacccaact cttgtgcaac
      961 tcatgccttg ctgagcgtgc tcctgaactg cagcagcgtg gacctgggac ccaccctgag
     1021 tcgcatgaag gacttcacca agggtttcag ccctgagagc aaaggatatg cgattggcaa
     1081 tgccccggag ttggccaagg cccataatag ccatgccagg cccgagccac gccacctccc
     1141 tgagaagcag aatggcctta gtgcagtgcg gaccatggag gcgttccact ttgtcagcta
     1201 tgtgcctatc acaggccggc tctttgagct ggatgggctg aaggtctacc ccattgacca
     1261 tgggccctgg ggggaggacg aggagtggac agacaaggcc cggcgggtca tcatggagcg
     1321 tatcggcctc gccactgcag gggagcccta ccacgacatc cgcttcaacc tgatggcagt
     1381 ggtgcccgac cgcaggatca agtatgaggc caggctgcat gtgctgaagg tgaaccgtca
     1441 gacagtacta gaggctctgc agcagctgat aagagtaaca cagccagagc tgattcagac
     1501 ccacaagtct caagagtcac agctgcctga ggagtccaag tcagccagca acaagtcccc
     1561 gctggtgctg gaagcaaaca gggcccctgc agcctctgag ggcaaccaca cagatggtgc
     1621 agaggaggcg gctggttcat gcgcacaagc cccatcccac agccctccca acaaacccaa
     1681 gctagtggtg aagcctccag gcagcagcct caatggggtt caccccaacc ccactcccat
     1741 tgtccagcgg ctgccggcct ttctagacaa tcacaattat gccaagtccc ccatgcagga
     1801 ggaagaagac ctggcggcag gtgtgggccg cagccgagtt ccagtccgcc caccccagca
     1861 gtactcagat gatgaggatg actatgagga tgacgaggag gatgacgtgc agaacaccaa
     1921 ctctgccctt aggtataagg ggaagggaac agggaagcca ggggcattga gcggttctgc
     1981 tgatgggcaa ctgtcagtgc tgcagcccaa caccatcaac gtcttggctg agaagctcaa
     2041 agagtcccag aaggacctct caattcctct gtccatcaag actagcagcg gggctgggag
     2101 tccggctgtg gcagtgccca cacactcgca gccctcaccc acccccagca atgagagtac
     2161 agacacggcc tctgagatcg gcagtgcttt caactcgcca ctgcgctcgc ctatccgctc
     2221 agccaacccg acgcggccct ccagccctgt cacctcccac atctccaagg tgctttttgg
     2281 agaggatgac agcctgctgc gtgttgactg catacgctac aaccgtgctg tccgtgatct
     2341 gggtcctgtc atcagcacag gcctgctgca cctggctgag gatggggtgc tgagtcccct
     2401 ggcgctgaca gagggtggga agggttcctc gccctccatc agaccaatcc aaggcagcca
     2461 ggggtccagc agcccagtgg agaaggaggt cgtggaagcc acggacagca gagagaagac
     2521 ggggatggtg aggcctggcg agcccttgag tggggagaaa tactcaccca aggagctgct
     2581 ggcactgctg aagtgtgtgg aggctgagat tgcaaactat gaggcgtgcc tcaaggagga
     2641 ggtagagaag aggaagaagt tcaagattga tgaccagaga aggacccaca actacgatga
     2701 gttcatctgc acctttatct ccatgctggc tcaggaaggc atgctggcca acctagtgga
     2761 gcagaacatc tccgtgcggc ggcgccaagg ggtcagcatc ggccggctcc acaagcagcg
     2821 gaagcctgac cggcggaaac gctctcgccc ctacaaggcc aagcgccagc tcgaggaaca
     2881 aaaactaata tcagaggaag atttggaaca gaaactaatc agtgaagaag atttagagca
     2941 aaagttaatt tccgaggagg acttatagtg agggcccgat atctcgacaa tcaacctctg
     3001 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     3061 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     3121 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     3181 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     3241 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     3301 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     3361 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     3421 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     3481 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     3541 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaaacggggg aggctaactg
     3601 aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga
     3661 ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg
     3721 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt
     3781 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg
     3841 gcaggccctg ccatagcaga tctgcgcagc tggggctcta gggggtatcc ccacgcgccc
     3901 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     3961 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     4021 ggctttcccc gtcaagctct aaatcggggc atccctttag ggttccgatt tagtgcttta
     4081 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     4141 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     4201 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     4261 ttggggattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     4321 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     4381 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     4441 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     4501 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     4561 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     4621 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     4681 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
     4741 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     4801 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     4861 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     4921 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     4981 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     5041 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     5101 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     5161 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     5221 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     5281 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     5341 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     5401 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     5461 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     5521 tctgagcggg actctggggt tcgcgaaatg accgaccaag cgacgcccaa cctgccatca
     5581 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     5641 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     5701 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     5761 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     5821 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     5881 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     5941 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     6001 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     6061 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     6121 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     6181 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     6241 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     6301 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     6361 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     6421 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt
     6481 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     6541 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     6601 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     6661 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     6721 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     6781 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     6841 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     6901 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     6961 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     7021 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     7081 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     7141 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     7201 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     7261 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     7321 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     7381 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     7441 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     7501 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     7561 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     7621 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     7681 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     7741 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     7801 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     7861 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     7921 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     7981 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga
     8041 gatctcccga tcccctatgg tcgactctca gtacaatctg ctctgatgcc gcatagttaa
     8101 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt
     8161 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agg
//
