LOCUS       V047137                12408 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V047137
VERSION     V047137
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        678..696
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             744..1451
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             1500..7088
                     /label="BRCA1(NM_007294)"
                     /note="BRCA1(NM_007294)"
                     /gene="BRCA1"
     CDS             7095..7160
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     misc_feature    7184..7772
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    7838..7886
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      8088..8516
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        8530..8859
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             8926..9720
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    9896..10017
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(10066..10082)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10090..10106
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10114..10144)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10159..10180
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10468..11056)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11227..12087)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12088..12192)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
      661 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta
      721 aacttaagct tggtaccgcc accatggtga gcaagggcga ggaggataac atggccatca
      781 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg
      841 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
      901 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg
      961 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc
     1021 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga
     1081 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca
     1141 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg
     1201 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga
     1261 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc
     1321 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact
     1381 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg
     1441 agctgtacaa gtggatcgga ggagggggaa gcggtggagg cggtagtgag ctcggatcca
     1501 tggatttatc tgctcttcgc gttgaagaag tacaaaatgt cattaatgct atgcagaaaa
     1561 tcttagagtg tcccatctgt ctggagttga tcaaggaacc tgtctccaca aagtgtgacc
     1621 acatattttg caaattttgc atgctgaaac ttctcaacca gaagaaaggg ccttcacagt
     1681 gtcctttatg taagaatgat ataaccaaaa ggagcctaca agaaagtacg agatttagtc
     1741 aacttgttga agagctattg aaaatcattt gtgcttttca gcttgacaca ggtttggagt
     1801 atgcaaacag ctataatttt gcaaaaaagg aaaataactc tcctgaacat ctaaaagatg
     1861 aagtttctat catccaaagt atgggctaca gaaaccgtgc caaaagactt ctacagagtg
     1921 aacccgaaaa tccttccttg caggaaacca gtctcagtgt ccaactctct aaccttggaa
     1981 ctgtgagaac tctgaggaca aagcagcgga tacaacctca aaagacgtct gtctacattg
     2041 aattgggatc tgattcttct gaagataccg ttaataaggc aacttattgc agtgtgggag
     2101 atcaagaatt gttacaaatc acccctcaag gaaccaggga tgaaatcagt ttggattctg
     2161 caaaaaaggc tgcttgtgaa ttttctgaga cggatgtaac aaatactgaa catcatcaac
     2221 ccagtaataa tgatttgaac accactgaga agcgtgcagc tgagaggcat ccagaaaagt
     2281 atcagggtag ttctgtttca aacttgcatg tggagccatg tggcacaaat actcatgcca
     2341 gctcattaca gcatgagaac agcagtttat tactcactaa agacagaatg aatgtagaaa
     2401 aggctgaatt ctgtaataaa agcaaacagc ctggcttagc aaggagccaa cataacagat
     2461 gggctggaag taaggaaaca tgtaatgata ggcggactcc cagcacagaa aaaaaggtag
     2521 atctgaatgc tgatcccctg tgtgagagaa aagaatggaa taagcagaaa ctgccatgct
     2581 cagagaatcc tagagatact gaagatgttc cttggataac actaaatagc agcattcaga
     2641 aagttaatga gtggttttcc agaagtgatg aactgttagg ttctgatgac tcacatgatg
     2701 gggagtctga atcaaatgcc aaagtagctg atgtattgga cgttctaaat gaggtagatg
     2761 aatattctgg ttcttcagag aaaatagact tactggccag tgatcctcat gaggctttaa
     2821 tatgtaaaag tgaaagagtt cactccaaat cagtagagag taatattgaa gacaaaatat
     2881 ttgggaaaac ctatcggaag aaggcaagcc tccccaactt aagccatgta actgaaaatc
     2941 taattatagg agcatttgtt actgagccac agataataca agagcgtccc ctcacaaata
     3001 aattaaagcg taaaaggaga cctacatcag gccttcatcc tgaggatttt atcaagaaag
     3061 cagatttggc agttcaaaag actcctgaaa tgataaatca gggaactaac caaacggagc
     3121 agaatggtca agtgatgaat attactaata gtggtcatga gaataaaaca aaaggtgatt
     3181 ctattcagaa tgagaaaaat cctaacccaa tagaatcact cgaaaaagaa tctgctttca
     3241 aaacgaaagc tgaacctata agcagcagta taagcaatat ggaactcgaa ttaaatatcc
     3301 acaattcaaa agcacctaaa aagaataggc tgaggaggaa gtcttctacc aggcatattc
     3361 atgcgcttga actagtagtc agtagaaatc taagcccacc taattgtact gaattgcaaa
     3421 ttgatagttg ttctagcagt gaagagataa agaaaaaaaa gtacaaccaa atgccagtca
     3481 ggcacagcag aaacctacaa ctcatggaag gtaaagaacc tgcaactgga gccaagaaga
     3541 gtaacaagcc aaatgaacag acaagtaaaa gacatgacag cgatactttc ccagagctga
     3601 agttaacaaa tgcacctggt tcttttacta agtgttcaaa taccagtgaa cttaaagaat
     3661 ttgtcaatcc tagccttcca agagaagaaa aagaagagaa actagaaaca gttaaagtgt
     3721 ctaataatgc tgaagacccc aaagatctca tgttaagtgg agaaagggtt ttgcaaactg
     3781 aaagatctgt agagagtagc agtatttcat tggtacctgg tactgattat ggcactcagg
     3841 aaagtatctc gttactggaa gttagcactc tagggaaggc aaaaacagaa ccaaataaat
     3901 gtgtgagtca gtgtgcagca tttgaaaacc ccaagggact aattcatggt tgttccaaag
     3961 ataatagaaa tgacacagaa ggctttaagt atccattggg acatgaagtt aaccacagtc
     4021 gggaaacaag catagaaatg gaagaaagtg aacttgatgc tcagtatttg cagaatacat
     4081 tcaaggtttc aaagcgccag tcatttgctc cgttttcaaa tccaggaaat gcagaagagg
     4141 aatgtgcaac attctctgcc cactctgggt ccttaaagaa acaaagtcca aaagtcactt
     4201 ttgaatgtga acaaaaggaa gaaaatcaag gaaagaatga gtctaatatc aagcctgtac
     4261 agacagttaa tatcactgca ggctttcctg tggttggtca gaaagataag ccagttgata
     4321 atgccaaatg tagtatcaaa ggaggctcta ggttttgtct atcatctcag ttcagaggca
     4381 acgaaactgg actcattact ccaaataaac atggactttt acaaaaccca tatcgtatac
     4441 caccactttt tcccatcaag tcatttgtta aaactaaatg taagaaaaat ctgctagagg
     4501 aaaactttga ggaacattca atgtcacctg aaagagaaat gggaaatgag aacattccaa
     4561 gtacagtgag cacaattagc cgtaataaca ttagagaaaa tgtttttaaa gaagccagct
     4621 caagcaatat taatgaagta ggttccagta ctaatgaagt gggctccagt attaatgaaa
     4681 taggttccag tgatgaaaac attcaagcag aactaggtag aaacagaggg ccaaaattga
     4741 atgctatgct tagattaggg gttttgcaac ctgaggtcta taaacaaagt cttcctggaa
     4801 gtaattgtaa gcatcctgaa ataaaaaagc aagaatatga agaagtagtt cagactgtta
     4861 atacagattt ctctccatat ctgatttcag ataacttaga acagcctatg ggaagtagtc
     4921 atgcatctca ggtttgttct gagacacctg atgacctgtt agatgatggt gaaataaagg
     4981 aagatactag ttttgctgaa aatgacatta aggaaagttc tgctgttttt agcaaaagcg
     5041 tccagaaagg agagcttagc aggagtccta gccctttcac ccatacacat ttggctcagg
     5101 gttaccgaag aggggccaag aaattagagt cctcagaaga gaacttatct agtgaggatg
     5161 aagagcttcc ctgcttccaa cacttgttat ttggtaaagt aaacaatata ccttctcagt
     5221 ctactaggca tagcaccgtt gctaccgagt gtctgtctaa gaacacagag gagaatttat
     5281 tatcattgaa gaatagctta aatgactgca gtaaccaggt aatattggca aaggcatctc
     5341 aggaacatca ccttagtgag gaaacaaaat gttctgctag cttgttttct tcacagtgca
     5401 gtgaattgga agacttgact gcaaatacaa acacccagga tcctttcttg attggttctt
     5461 ccaaacaaat gaggcatcag tctgaaagcc agggagttgg tctgagtgac aaggaattgg
     5521 tttcagatga tgaagaaaga ggaacgggct tggaagaaaa taatcaagaa gagcaaagca
     5581 tggattcaaa cttaggtgaa gcagcatctg ggtgtgagag tgaaacaagc gtctctgaag
     5641 actgctcagg gctatcctct cagagtgaca ttttaaccac tcagcagagg gataccatgc
     5701 aacataacct gataaagctc cagcaggaaa tggctgaact agaagctgtg ttagaacagc
     5761 atgggagcca gccttctaac agctaccctt ccatcataag tgactcttct gcccttgagg
     5821 acctgcgaaa tccagaacaa agcacatcag aaaaagcagt attaacttca cagaaaagta
     5881 gtgaataccc tataagccag aatccagaag gcctttctgc tgacaagttt gaggtgtctg
     5941 cagatagttc taccagtaaa aataaagaac caggagtgga aaggtcatcc ccttctaaat
     6001 gcccatcatt agatgatagg tggtacatgc acagttgctc tgggagtctt cagaatagaa
     6061 actacccatc tcaagaggag ctcattaagg ttgttgatgt ggaggagcaa cagctggaag
     6121 agtctgggcc acacgatttg acggaaacat cttacttgcc aaggcaagat ctagagggaa
     6181 ccccttacct ggaatctgga atcagcctct tctctgatga ccctgaatct gatccttctg
     6241 aagacagagc cccagagtca gctcgtgttg gcaacatacc atcttcaacc tctgcattga
     6301 aagttcccca attgaaagtt gcagaatctg cccagagtcc agctgctgct catactactg
     6361 atactgctgg gtataatgca atggaagaaa gtgtgagcag ggagaagcca gaattgacag
     6421 cttcaacaga aagggtcaac aaaagaatgt ccatggtggt gtctggcctg accccagaag
     6481 aatttatgct cgtgtacaag tttgccagaa aacaccacat cactttaact aatctaatta
     6541 ctgaagagac tactcatgtt gttatgaaaa cagatgctga gtttgtgtgt gaacggacac
     6601 tgaaatattt tctaggaatt gcgggaggaa aatgggtagt tagctatttc tgggtgaccc
     6661 agtctattaa agaaagaaaa atgctgaatg agcatgattt tgaagtcaga ggagatgtgg
     6721 tcaatggaag aaaccaccaa ggtccaaagc gagcaagaga atcccaggac agaaagatct
     6781 tcagggggct agaaatctgt tgctatgggc ccttcaccaa catgcccaca gatcaactgg
     6841 aatggatggt acagctgtgt ggtgcttctg tggtgaagga gctttcatca ttcacccttg
     6901 gcacaggtgt ccacccaatt gtggttgtgc agccagatgc ctggacagag gacaatggct
     6961 tccatgcaat tgggcagatg tgtgaggcac ctgtggtgac ccgagagtgg gtgttggaca
     7021 gtgtagcact ctaccagtgc caggagctgg acacctacct gataccccag atcccccaca
     7081 gccactacct cgaggactac aaagaccatg acggtgatta taaagatcat gacatcgact
     7141 acaaggatga cgatgacaag tagtgagggc ccgatatctc gacaatcaac ctctggatta
     7201 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     7261 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     7321 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     7381 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     7441 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     7501 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     7561 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg
     7621 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     7681 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     7741 gagtcggatc tccctttggg ccgcctcccc gcctggaaac gggggaggct aactgaaaca
     7801 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa
     7861 acgcacgggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc
     7921 tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc
     7981 accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg
     8041 ccctgccata gcagatctgc gcagctgggg ctctaggggg tatccccacg cgccctgtag
     8101 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     8161 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     8221 tccccgtcaa gctctaaatc ggggcatccc tttagggttc cgatttagtg ctttacggca
     8281 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     8341 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     8401 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttggg
     8461 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     8521 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     8581 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     8641 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     8701 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     8761 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     8821 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     8881 tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     8941 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     9001 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     9061 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
     9121 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     9181 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     9241 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     9301 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     9361 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     9421 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
     9481 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     9541 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     9601 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     9661 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     9721 gcgggactct ggggttcgcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     9781 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     9841 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     9901 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     9961 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
    10021 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
    10081 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
    10141 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
    10201 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
    10261 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
    10321 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
    10381 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
    10441 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
    10501 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
    10561 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
    10621 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta
    10681 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
    10741 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
    10801 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
    10861 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
    10921 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
    10981 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
    11041 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
    11101 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
    11161 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
    11221 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
    11281 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
    11341 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
    11401 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
    11461 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
    11521 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
    11581 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
    11641 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
    11701 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
    11761 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
    11821 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
    11881 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
    11941 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
    12001 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    12061 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    12121 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    12181 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct
    12241 cccgatcccc tatggtcgac tctcagtaca atctgctctg atgccgcata gttaagccag
    12301 tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct
    12361 acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagg
//
