LOCUS       V029934                 5883 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029934
VERSION     V029934
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2030
                     /label="Bdkrb1(NM_007539)"
                     /note="Bdkrb1(NM_007539)"
                     /gene="Bdkrb1"
     CDS             2049..2078
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2097..2120
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2140..2156)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2167..2789
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(2938..3526)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    3855..3902
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4134..4928)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(4963..5320)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5322..5426)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5453..5881
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggcgtcccag gcctcgctga agctacagcc ttctaaccaa agccagcagg
     1081 cccctcccaa catcacctcc tgcgagggcg ccccggaagc ctgggatctg ctgtgtcggg
     1141 tgctgccagg gtttgtcatc actgtctgtt tctttggcct cctggggaac cttttagtcc
     1201 tgtccttctt ccttttgcct tggcgacgat ggtggcagca gcggcggcag cgcctaacca
     1261 tagcagaaat ctacctggct aacttggcag cttctgatct ggtgtttgtg ctgggcctgc
     1321 ccttctgggc agagaacgtt gggaaccgtt tcaactggcc ctttggaagt gacctctgcc
     1381 gggtggtcag cggggtcatc aaggccaacc tgttcatcag catcttcctg gtggtggcca
     1441 tcagtcagga ccgctacagg ttgctggtat accccatgac cagctggggg aaccggcggc
     1501 gacggcaagc ccaagtgacc tgcctgctca tctgggtagc tgggggcctc ttgagcaccc
     1561 ccacgttcct tctgcgttcc gtcaaagtcg tccctgatct gaacatctct gcctgcatcc
     1621 tgcttttccc ccacgaagct tggcactttg taaggatggt ggagttgaac gttttgggtt
     1681 tcctcctccc attggctgcc atcctctact tcaactttca catcctggcc tccctgagag
     1741 gacagaagga ggccagcaga acccggtgtg ggggacccaa ggacagcaag acaatggggc
     1801 tgatcctcac actggtagcc tccttcctgg tctgctgggc cccttaccac ttctttgcct
     1861 tcctggattt cctggtccag gtgagagtga tccaggactg cttctggaag gagctcacag
     1921 acctgggcct gcagctggcc aacttctttg cttttgtcaa cagctgcctg aacccactga
     1981 tttatgtctt tgcaggccgg ctctttaaga ccagggttct gggaacttta acgcgtacgc
     2041 ggccgctcga gcagaaactc atctcagaag aggatctggc agcaaatgat atcctggatt
     2101 acaaggatga cgacgataag gtttaaacgg ccggccgcgg tcatagctgt ttcctgaaca
     2161 gatcccgggt ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc
     2221 actccagtgc ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag
     2281 gtgtccttct ataatattat ggggtggagg ggggtggtat ggagcaaggg gcaagttggg
     2341 aagacaacct gtagggcctg cggggtctat tgggaaccaa gctggagtgc agtggcacaa
     2401 tcttggctca ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc
     2461 gagttgttgg gattccaggc atgcatgacc aggctcagct aatttttgtt tttttggtag
     2521 agacggggtt tcaccatatt ggccaggctg gtctccaact cctaatctca ggtgatctac
     2581 ccaccttggc ctcccaaatt gctgggatta caggcgtgaa ccactgctcc cttccctgtc
     2641 cttctgattt taaaataact ataccagcag gaggacgtcc agacacagca taggctacct
     2701 ggccatgccc aaccggtggg acatttgagt tgcttgcttg gcactgtcct ctcatgcgtt
     2761 gggtccactc agtagatgcc tgttgaattg ggtacgcggc cagcggcgag cggtatcagc
     2821 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     2881 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     2941 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     3001 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     3061 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     3121 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     3181 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     3241 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     3301 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     3361 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     3421 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     3481 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     3541 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     3601 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     3661 aatctaaagt atatatgagt aacctgaggc tatggcaggg cctgccgccc cgacgttggc
     3721 tgcgagccct gggccttcac ccgaacttgg ggggtggggt ggggaaaagg aagaaacgcg
     3781 ggcgtattgg ccccaatggg gtctcggtgg ggtatcgaca gagtgccagc cctgggaccg
     3841 aaccccgcgt ttatgaacaa acgacccaac accgtgcgtt ttattctgtc tttttattgc
     3901 cgtcatagcg cgggttcctt ccggtattgt ctccttccgt gtttcagtta gcctccccct
     3961 agggtgggcg aagaactcca gcatgagatc cccgcgctgg aggatcatcc agccggcgtc
     4021 ccggaaaacg attccgaagc ccaacctttc atagaaggcg gcggtggaat cgaaatctcg
     4081 tgatggcagg ttgggcgtcg cttggtcggt catttcgaac cccagagtcc cgctcagaag
     4141 aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa
     4201 agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc acgggtagcc
     4261 aacgctatgt cctgatagcg atccgccaca cccagccggc cacagtcgat gaatccagaa
     4321 aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgggt cacgacgaga
     4381 tcctcgccgt cgggcatgct cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc
     4441 tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg agtacgtgct
     4501 cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc aagcgtatgc
     4561 agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag gtgagatgac
     4621 aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc ttcagtgaca
     4681 acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc
     4741 tcgtcttgca gttcattcag ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc
     4801 ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg ttgtgcccag
     4861 tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt
     4921 tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcga tctttgcaaa agcctaggcc
     4981 tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc
     5041 tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt
     5101 taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat tgagatgcat
     5161 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacctgg ttgctgacta
     5221 attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacaccc
     5281 taactgacac acattccaca gctggttctt tccgcctcag gactcttcct ttttcaatat
     5341 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     5401 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc
     5461 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     5521 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     5581 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     5641 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     5701 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     5761 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     5821 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     5881 ttt
//
