LOCUS       V031046                 6057 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031046
VERSION     V031046
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2204
                     /label="Bdkrb2(NM_009747)"
                     /note="Bdkrb2(NM_009747)"
                     /gene="Bdkrb2"
     CDS             2223..2252
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2271..2294
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2314..2330)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2341..2963
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3112..3700)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4029..4076
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4308..5102)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5137..5494)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5496..5600)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5627..6055
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gccctgctcc tggaagctac tcgggtttct gtcggtgcat gagcccatgc
     1081 ccaccgcggc ctcctttggc atcgaaatgt tcaacgtcac cacacaagtc ctcgggtctg
     1141 ctcttaacgg gaccctttcg aaggacaact gcccagacac cgagtggtgg agttggctca
     1201 atgccatcca ggcccccttc ctctgggtcc tcttcctgct ggccgcactg gagaacctct
     1261 ttgtcctcag cgtgttcttc ctgcacaaaa acagctgcac tgtggccgag atctacctgg
     1321 gcaacctggc agcggcggac ctcatcctgg cctgcgggtt acctttctgg gccatcacca
     1381 tcgccaataa ctttgactgg gtgtttggag aggtgttgtg ccgggtggtg aacaccatga
     1441 tctacatgaa cctgtacagc agcatctgct tcctgatgct cgtgagtatc gaccgctacc
     1501 tggcgctggt gaagaccatg tccatgggcc ggatgcgcgg ggtgcgctgg gccaaactct
     1561 acagcctggt gatctggggc tgtacactgc ttctgagttc acccatgttg gtgttcagga
     1621 ccatgaggga atacagcgaa gagggccaca atgtcaccgc ctgcgtcatc gtctacccgt
     1681 cccgttcctg ggaggtgttc accaacgtgc tgctgaacct ggtgggtttc ctcctgcccc
     1741 tgagcgtcat caccttctgc acggtgcgca tcttgcaggt gctgaggaac aacgagatga
     1801 agaagttcaa ggaggtccag acggagagga aggccaccgt gctagtgctg gccgtcctgg
     1861 ggctctttgt gctgtgttgg gtgcctttcc agatcagcac cttcctggac acgctgctgc
     1921 gcctcggcgt gctgtccgga tgctgggacg agcacgccgt agacgtcatc acgcagatca
     1981 gttcctacgt ggcctacagc aacagcggcc tcaacccact ggtgtacgtg atcgtgggca
     2041 agcgcttccg gaagaagtcc cgagaggtgt accgggtgct gtgccagaaa ggaggctgca
     2101 tgggagaacc cgtccagatg gagaactcca tggggacttt gagaacctcg atctccgtgg
     2161 aacggcagat ccacaagctg caggactggg cagggaagaa acagacgcgt acgcggccgc
     2221 tcgagcagaa actcatctca gaagaggatc tggcagcaaa tgatatcctg gattacaagg
     2281 atgacgacga taaggtttaa acggccggcc gcggtcatag ctgtttcctg aacagatccc
     2341 gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca
     2401 gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc
     2461 ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca
     2521 acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg
     2581 ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg
     2641 ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg
     2701 ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct
     2761 tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg
     2821 attttaaaat aactatacca gcaggaggac gtccagacac agcataggct acctggccat
     2881 gcccaaccgg tgggacattt gagttgcttg cttggcactg tcctctcatg cgttgggtcc
     2941 actcagtaga tgcctgttga attgggtacg cggccagcgg cgagcggtat cagctcactc
     3001 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     3061 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     3121 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     3181 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     3241 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     3301 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     3361 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     3421 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     3481 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     3541 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     3601 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     3661 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     3721 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     3781 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     3841 aagtatatat gagtaacctg aggctatggc agggcctgcc gccccgacgt tggctgcgag
     3901 ccctgggcct tcacccgaac ttggggggtg gggtggggaa aaggaagaaa cgcgggcgta
     3961 ttggccccaa tggggtctcg gtggggtatc gacagagtgc cagccctggg accgaacccc
     4021 gcgtttatga acaaacgacc caacaccgtg cgttttattc tgtcttttta ttgccgtcat
     4081 agcgcgggtt ccttccggta ttgtctcctt ccgtgtttca gttagcctcc ccctagggtg
     4141 ggcgaagaac tccagcatga gatccccgcg ctggaggatc atccagccgg cgtcccggaa
     4201 aacgattccg aagcccaacc tttcatagaa ggcggcggtg gaatcgaaat ctcgtgatgg
     4261 caggttgggc gtcgcttggt cggtcatttc gaaccccaga gtcccgctca gaagaactcg
     4321 tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg
     4381 aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct
     4441 atgtcctgat agcgatccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg
     4501 ccattttcca ccatgatatt cggcaagcag gcatcgccat gggtcacgac gagatcctcg
     4561 ccgtcgggca tgctcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc
     4621 tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg
     4681 atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc
     4741 cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga
     4801 tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg
     4861 agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct
     4921 tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc
     4981 gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag
     5041 ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc
     5101 atgcgaaacg atcctcatcc tgtctcttga tcgatctttg caaaagccta ggcctccaaa
     5161 aaagcctcct cactacttct ggaatagctc agaggccgag gcggcctcgg cctctgcata
     5221 aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg
     5281 cgggatgggc ggagttaggg gcgggactat ggttgctgac taattgagat gcatgctttg
     5341 catacttctg cctgctgggg agcctgggga ctttccacac ctggttgctg actaattgag
     5401 atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac accctaactg
     5461 acacacattc cacagctggt tctttccgcc tcaggactct tcctttttca atattattga
     5521 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     5581 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgc gccctgtagc
     5641 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     5701 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     5761 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     5821 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     5881 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     5941 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     6001 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaatttt
//
