LOCUS       V030982                 5349 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030982
VERSION     V030982
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..721
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..917
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        946..964
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1023..1496
                     /label="Btg2(NM_007570)"
                     /note="Btg2(NM_007570)"
                     /gene="Btg2"
     CDS             1515..1544
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             1563..1586
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(1606..1622)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    1633..2255
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(2404..2992)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    3321..3368
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(3600..4394)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(4429..4786)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(4788..4892)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      4919..5347
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt catctacgta ttagtcatcg ctattaccat
      721 gggatgcggt ttggcagtcc ccaatgggcg tggatagcgg ttgactcacg gggatttcca
      781 agtctccacc ccattgacgt caatgggagt ttgtttggca ccaaaatcaa cgggactttc
      841 caaaatgtcg taataacccc gccccgttga cgcaaatggg cggtaggcgt gtacggtggg
      901 aggtctatat aagcagagct cgtttagtga accgtcagaa ttttgtaata cgactcacta
      961 tagggcggcc gggaattcgt cgactggatc cggtaccgag gagatctgcc gccgcgatcg
     1021 ccatgagcca cgggaagaga accgacatgc tcccggagat cgccgccgcc gtgggtttcc
     1081 tctccagtct cctgaggact cggggctgcg tgagcgagca gagactcaag gttttcagta
     1141 gggcgctcca ggacgcactg accgatcatt acaaacacca ctggtttcca gaaaaaccat
     1201 ccaaaggttc tggctatcgc tgtatccgta tcaaccacaa gatggacccc atcatcagca
     1261 aggtggccag ccagattgga ctcagccagc cccagctgca ccggctgctg cccagtgagc
     1321 tgaccctgtg ggttgatccc tatgaagtgt cttaccgcat cggggaggat ggctccatct
     1381 gtgtcctgta tgaggaggcc ccggtggctg cctcctatgg gctcctcacc tgcaagaacc
     1441 agatgatgct gggcaggagc agcccctcga agaactatgt gatggccgtc tccagcacgc
     1501 gtacgcggcc gctcgagcag aaactcatct cagaagagga tctggcagca aatgatatcc
     1561 tggattacaa ggatgacgac gataaggttt aaacggccgg ccgcggtcat agctgtttcc
     1621 tgaacagatc ccgggtggca tccctgtgac ccctccccag tgcctctcct ggccctggaa
     1681 gttgccactc cagtgcccac cagccttgtc ctaataaaat taagttgcat cattttgtct
     1741 gactaggtgt ccttctataa tattatgggg tggagggggg tggtatggag caaggggcaa
     1801 gttgggaaga caacctgtag ggcctgcggg gtctattggg aaccaagctg gagtgcagtg
     1861 gcacaatctt ggctcactgc aatctccgcc tcctgggttc aagcgattct cctgcctcag
     1921 cctcccgagt tgttgggatt ccaggcatgc atgaccaggc tcagctaatt tttgtttttt
     1981 tggtagagac ggggtttcac catattggcc aggctggtct ccaactccta atctcaggtg
     2041 atctacccac cttggcctcc caaattgctg ggattacagg cgtgaaccac tgctcccttc
     2101 cctgtccttc tgattttaaa ataactatac cagcaggagg acgtccagac acagcatagg
     2161 ctacctggcc atgcccaacc ggtgggacat ttgagttgct tgcttggcac tgtcctctca
     2221 tgcgttgggt ccactcagta gatgcctgtt gaattgggta cgcggccagc ggcgagcggt
     2281 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     2341 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     2401 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     2461 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     2521 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     2581 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     2641 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     2701 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     2761 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     2821 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     2881 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     2941 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     3001 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     3061 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     3121 taaatcaatc taaagtatat atgagtaacc tgaggctatg gcagggcctg ccgccccgac
     3181 gttggctgcg agccctgggc cttcacccga acttgggggg tggggtgggg aaaaggaaga
     3241 aacgcgggcg tattggcccc aatggggtct cggtggggta tcgacagagt gccagccctg
     3301 ggaccgaacc ccgcgtttat gaacaaacga cccaacaccg tgcgttttat tctgtctttt
     3361 tattgccgtc atagcgcggg ttccttccgg tattgtctcc ttccgtgttt cagttagcct
     3421 ccccctaggg tgggcgaaga actccagcat gagatccccg cgctggagga tcatccagcc
     3481 ggcgtcccgg aaaacgattc cgaagcccaa cctttcatag aaggcggcgg tggaatcgaa
     3541 atctcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct
     3601 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     3661 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     3721 gtagccaacg ctatgtcctg atagcgatcc gccacaccca gccggccaca gtcgatgaat
     3781 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     3841 acgagatcct cgccgtcggg catgctcgcc ttgagcctgg cgaacagttc ggctggcgcg
     3901 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     3961 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     4021 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     4081 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     4141 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     4201 gctgcctcgt cttgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     4261 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     4321 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     4381 tcttgttcaa tcatgcgaaa cgatcctcat cctgtctctt gatcgatctt tgcaaaagcc
     4441 taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggccg aggcggcctc
     4501 ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg
     4561 cggagttagg ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag
     4621 atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc
     4681 tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc
     4741 acaccctaac tgacacacat tccacagctg gttctttccg cctcaggact cttccttttt
     4801 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     4861 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     4921 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     4981 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     5041 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     5101 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     5161 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     5221 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     5281 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     5341 gcgaatttt
//
