LOCUS V022546 7436 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V022546 VERSION V022546 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 683..2095 /label="CADM1(NM_001301043)" /note="CADM1(NM_001301043)" /gene="CADM1" CDS 2102..2128 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 2132..2158 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 2162..2188 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" misc_feature 2212..2800 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 2866..2914 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 3116..3544 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3558..3887 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3954..4748 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4924..5045 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5094..5110) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5118..5134 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5142..5172) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5187..5208 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5496..6084) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6255..7115) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7116..7220) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg 661 taccgagctc ggatccgcca ccatggcgag tgtagtgctg ccgagcggat cccagtgtgc 721 ggcggcagcg gcggcggcgg cgcctcccgg gctccggctc cggcttctgc tgttgctctt 781 ctccgccgcg gcactgatcc ccacaggtga tgggcagaat ctgtttacga aagacgtgac 841 agtgatcgag ggagaggttg cgaccatcag ttgccaagtc aataagagtg acgactctgt 901 gattcagcta ctgaatccca acaggcagac catttatttc agggacttca ggcctttgaa 961 ggacagcagg tttcagttgc tgaatttttc tagcagtgaa ctcaaagtat cattgacaaa 1021 cgtctcaatt tctgatgaag gaagatactt ttgccagctc tataccgatc ccccacagga 1081 aagttacacc accatcacag tcctggtccc accacgtaat ctgatgatcg atatccagaa 1141 agacactgcg gtggaaggtg aggagattga agtcaactgc actgctatgg ccagcaagcc 1201 agccacgact atcaggtggt tcaaagggaa cacagagcta aaaggcaaat cggaggtgga 1261 agagtggtca gacatgtaca ctgtgaccag tcagctgatg ctgaaggtgc acaaggagga 1321 cgatggggtc ccagtgatct gccaggtgga gcaccctgcg gtcactggaa acctgcagac 1381 ccagcggtat ctagaagtac agtataagcc tcaagtgcac attcagatga cttatcctct 1441 acaaggctta acccgggaag gggacgcgct tgagttaaca tgtgaagcca tcgggaagcc 1501 ccagcctgtg atggtaactt gggtgagagt cgatgatgaa atgcctcaac acgccgtact 1561 gtctgggccc aacctgttca tcaataacct aaacaaaaca gataatggta cataccgctg 1621 tgaagcttca aacatagtgg ggaaagctca ctcggattat atgctgtatg tatacgatcc 1681 ccccacaact atccctcctc ccacaacaac caccaccacc accaccacca ccaccaccac 1741 catccttacc atcatcacag acacaacggc gacgacagaa ccagcagttc acggccttac 1801 tcagttgccc aattccgcag aagaactgga cagtgaggac ctctcagatt cccgagcagg 1861 tgaagaaggc tcgatcaggg cagtggatca tgccgtgatc ggtggcgtcg tggcggtggt 1921 ggtgttcgcc atgctgtgct tgctcatcat tctggggcgc tattttgcca gacataaagg 1981 tacatacttc actcatgaag ccaaaggagc cgatgacgca gcagacgcag acacagctat 2041 aatcaatgca gaaggaggac agaacaactc cgaagaaaag aaagagtact tcatcctcga 2101 gtacccatac gacgtacctg attatgcaac ttatccttat gatgtacctg attatgctag 2161 ctatccttat gatgtcccag actacgcata gtgagggccc gatatctcga caatcaacct 2221 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg 2281 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc 2341 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt 2401 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc 2461 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg 2521 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact 2581 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt 2641 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg 2701 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc 2761 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaaacgg gggaggctaa 2821 ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac 2881 agaataaaac gcacgggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc 2941 tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc 3001 ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg 3061 gcggcaggcc ctgccatagc agatctgcgc agctggggct ctagggggta tccccacgcg 3121 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca 3181 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc 3241 gccggctttc cccgtcaagc tctaaatcgg ggcatccctt tagggttccg atttagtgct 3301 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg 3361 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc 3421 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg 3481 attttgggga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg 3541 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag 3601 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag 3661 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc 3721 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 3781 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat 3841 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag 3901 cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg 3961 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg 4021 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg 4081 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg 4141 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg 4201 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc 4261 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc 4321 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc 4381 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc 4441 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg 4501 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct 4561 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt 4621 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc 4681 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt 4741 tcttctgagc gggactctgg ggttcgcgaa atgaccgacc aagcgacgcc caacctgcca 4801 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc 4861 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac 4921 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 4981 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 5041 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag 5101 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc 5161 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc 5221 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa 5281 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg 5341 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 5401 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 5461 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 5521 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 5581 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 5641 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc 5701 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 5761 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 5821 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 5881 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 5941 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 6001 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 6061 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 6121 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 6181 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 6241 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 6301 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 6361 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 6421 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 6481 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 6541 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt 6601 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 6661 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 6721 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 6781 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 6841 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 6901 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 6961 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 7021 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 7081 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 7141 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 7201 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg 7261 ggagatctcc cgatccccta tggtcgactc tcagtacaat ctgctctgat gccgcatagt 7321 taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa 7381 tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagg //