LOCUS       V018285                 6230 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V018285
VERSION     V018285
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1055..2401
                     /label="CLU(NM_001831)"
                     /note="CLU(NM_001831)"
                     /gene="CLU"
     CDS             2408..2425
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /transl_table=1
                     /translation="HHHHHH"
                     /label="6xHis"
     CDS             2444..2467
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2487..2503)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2514..3136
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3285..3873)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4202..4249
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4481..5275)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5310..5667)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5669..5773)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5800..6228
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgcggc gcgccagatc tcaagcttgc caccatgatg aagactctgc tgctgtttgt
     1081 ggggctgctg ctgacctggg agagtgggca ggtcctgggg gaccagacgg tctcagacaa
     1141 tgagctccag gaaatgtcca atcagggaag taagtacgtc aataaggaaa ttcaaaatgc
     1201 tgtcaacggg gtgaaacaga taaagactct catagaaaaa acaaacgaag agcgcaagac
     1261 actgctcagc aacctagaag aagccaagaa gaagaaagag gatgccctaa atgagaccag
     1321 ggaatcagag acaaagctga aggagctccc aggagtgtgc aatgagacca tgatggccct
     1381 ctgggaagag tgtaagccct gcctgaaaca gacctgcatg aagttctacg cacgcgtctg
     1441 cagaagtggc tcaggcctgg ttggccgcca gcttgaggag ttcctgaacc agagctcgcc
     1501 cttctacttc tggatgaatg gtgaccgcat cgactccctg ctggagaacg accggcagca
     1561 gacgcacatg ctggatgtca tgcaggacca cttcagccgc gcgtccagca tcatagacga
     1621 gctcttccag gacaggttct tcacccggga gccccaggat acctaccact acctgccctt
     1681 cagcctgccc caccggaggc ctcacttctt ctttcccaag tcccgcatcg tccgcagctt
     1741 gatgcccttc tctccgtacg agcccctgaa cttccacgcc atgttccagc ccttccttga
     1801 gatgatacac gaggctcagc aggccatgga catccacttc catagcccgg ccttccagca
     1861 cccgccaaca gaattcatac gagaaggcga cgatgaccgg actgtgtgcc gggagatccg
     1921 ccacaactcc acgggctgcc tgcggatgaa ggaccagtgt gacaagtgcc gggagatctt
     1981 gtctgtggac tgttccacca acaacccctc ccaggctaag ctgcggcggg agctcgacga
     2041 atccctccag gtcgctgaga ggttgaccag gaaatacaac gagctgctaa agtcctacca
     2101 gtggaagatg ctcaacacct cctccttgct ggagcagctg aacgagcagt ttaactgggt
     2161 gtcccggctg gcaaacctca cgcaaggcga agaccagtac tatctgcggg tcaccacggt
     2221 ggcttcccac acttctgact cggacgttcc ttccggtgtc actgaggtgg tcgtgaagct
     2281 ctttgactct gatcccatca ctgtgacggt ccctgtagaa gtctccagga agaaccctaa
     2341 atttatggag accgtggcgg agaaagcgct gcaggaatac cgcaaaaagc accgggagga
     2401 gctcgagcat catcatcatc atcacgcagc aaatgatatc ctggattaca aggatgacga
     2461 cgataaggtt taaacggccg gccgcggtca tagctgtttc ctgaacagat cccgggtggc
     2521 atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact ccagtgccca
     2581 ccagccttgt cctaataaaa ttaagttgca tcattttgtc tgactaggtg tccttctata
     2641 atattatggg gtggaggggg gtggtatgga gcaaggggca agttgggaag acaacctgta
     2701 gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt ggcacaatct tggctcactg
     2761 caatctccgc ctcctgggtt caagcgattc tcctgcctca gcctcccgag ttgttgggat
     2821 tccaggcatg catgaccagg ctcagctaat ttttgttttt ttggtagaga cggggtttca
     2881 ccatattggc caggctggtc tccaactcct aatctcaggt gatctaccca ccttggcctc
     2941 ccaaattgct gggattacag gcgtgaacca ctgctccctt ccctgtcctt ctgattttaa
     3001 aataactata ccagcaggag gacgtccaga cacagcatag gctacctggc catgcccaac
     3061 cggtgggaca tttgagttgc ttgcttggca ctgtcctctc atgcgttggg tccactcagt
     3121 agatgcctgt tgaattgggt acgcggccag cggcgagcgg tatcagctca ctcaaaggcg
     3181 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     3241 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     3301 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     3361 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     3421 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     3481 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     3541 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     3601 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     3661 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     3721 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     3781 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     3841 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     3901 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     3961 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     4021 tatgagtaac ctgaggctat ggcagggcct gccgccccga cgttggctgc gagccctggg
     4081 ccttcacccg aacttggggg gtggggtggg gaaaaggaag aaacgcgggc gtattggccc
     4141 caatggggtc tcggtggggt atcgacagag tgccagccct gggaccgaac cccgcgttta
     4201 tgaacaaacg acccaacacc gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg
     4261 gttccttccg gtattgtctc cttccgtgtt tcagttagcc tccccctagg gtgggcgaag
     4321 aactccagca tgagatcccc gcgctggagg atcatccagc cggcgtcccg gaaaacgatt
     4381 ccgaagccca acctttcata gaaggcggcg gtggaatcga aatctcgtga tggcaggttg
     4441 ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa
     4501 ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc
     4561 ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct
     4621 gatagcgatc cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt
     4681 ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg
     4741 gcatgctcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt
     4801 ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat
     4861 gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg
     4921 catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc
     4981 ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag
     5041 ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcttgcagtt
     5101 cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca
     5161 gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata
     5221 gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa
     5281 acgatcctca tcctgtctct tgatcgatct ttgcaaaagc ctaggcctcc aaaaaagcct
     5341 cctcactact tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa
     5401 aaaattagtc agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg
     5461 ggcggagtta ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt
     5521 ctgcctgctg gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg
     5581 ctttgcatac ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca
     5641 ttccacagct ggttctttcc gcctcaggac tcttcctttt tcaatattat tgaagcattt
     5701 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     5761 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat
     5821 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     5881 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     5941 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     6001 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     6061 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     6121 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     6181 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
//
