LOCUS V046964 9721 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V046964 VERSION V046964 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 706..4380 /label="CTNND2(NM_001332)" /note="CTNND2(NM_001332)" /gene="CTNND2" CDS 4387..4413 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 4417..4443 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 4447..4473 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" misc_feature 4497..5085 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 5151..5199 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 5401..5829 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 5843..6172 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 6239..7033 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 7209..7330 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(7379..7395) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7403..7419 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7427..7457) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7472..7493 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7781..8369) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8540..9400) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9401..9505) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg 661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgtt tgcgaggaag 721 ccgccgggcg ccgcgccttt gggagctatg cctgttccag accagccttc atcagcctca 781 gagaagacga gttccctgag ccccggctta aacacctcca acggggatgg ctctgaaaca 841 gaaaccacct ctgccatcct cgcctcagtc aaagaacagg aattacagtt tgaaaggctg 901 acccgagagc tggaggctga acggcagatc gtagccagcc agctggagcg atgcaagctc 961 ggatccgaga ctggcagcat gagcagcatg agttcagcag aagagcagtt tcagtggcag 1021 tcacaagatg gtcaaaaaga tatcgaagat gagcttacaa caggtctcga gctggtggac 1081 tcctgtatta ggtcactaca ggaatcagga atacttgacc cacaggatta ttctacaggt 1141 gaaaggccca gcctgctctc ccagagtgca cttcagctca attccaaacc tgaagggtct 1201 ttccagtatc cggccagcta ccatagcaac cagaccctgg ccctggggga aaccacccct 1261 tcacagctcc cggcccgagg cacacaagcc cgagctacgg gccagagctt cagccagggc 1321 acgaccagcc gcgccggcca cctggcgggg cccgagcccg cgccgccgcc gccgccgccg 1381 ccgcgggagc cgttcgcgcc cagcctgggc agcgccttcc acctgcccga cgcgccgccc 1441 gccgccgccg ccgccgcgct ctactactcc agctccacgc tgcccgcgcc gccgcgcggg 1501 ggctccccgc tggccgcgcc ccagggcggt tcgcccacca agctgcagcg cggcggctcg 1561 gcccccgagg gcgccaccta cgccgcgccg cgcggctcct cgcccaagca gtcgcccagc 1621 cgcctggcca agtcctacag caccagctcg cccatcaaca tcgtcgtgtc ctcggccggc 1681 ctgtccccga tccgcgtgac ctcgcccccc accgtgcagt ccaccatctc ctcctcgccc 1741 atccaccagc tgagctccac catcggcacg tacgccaccc tgtcgcccac caagcgcctg 1801 gtccacgcgt ccgagcagta cagcaagcac tcgcaggagc tgtatgccac ggccaccctc 1861 cagaggccgg gcagcctggc agctggttcc cgagcctcat acagcagcca gcatgggcac 1921 ctgggcccag agttgcgggc cctgcagtcc ccagaacacc acatagatcc catctatgaa 1981 gaccgcgtct atcagaagcc ccctatgagg agtctcagcc agagccaggg ggaccctctg 2041 ccgccagcac acaccggcac ctaccgcacg agcacagccc catcttcccc tggtgtcgac 2101 tccgtcccct tgcagcgcac aggcagccag cacggcccac agaatgccgc cgcggccacc 2161 ttccagaggg ccagctatgc cgccggccca gcctccaatt acgcggaccc ctaccgacag 2221 ctgcagtatt gtccctctgt tgagtctcca tacagcaaat ccggccctgc tctcccgcct 2281 gaaggcacct tggccaggtc cccgtccatt gatagcattc agaaagatcc cagagaattt 2341 ggatggagag acccggaact gccggaagtg attcagatgt tgcagcacca gtttccctcg 2401 gtccagtcta acgcggcagc ctacttgcaa cacctctgtt ttggagacaa caaaattaaa 2461 gccgagataa ggagacaagg aggcatccag ctcctggtgg acctgttgga tcatcggatg 2521 accgaagtcc accgtagtgc ctgtggagct ctgagaaacc tggtgtatgg gaaggccaac 2581 gatgataaca aaattgccct gaaaaactgt ggtggcatcc cagcactggt gaggttactc 2641 cgcaagacga ctgacctgga gatccgggag ctggtcacag gagtcctttg gaacctctcc 2701 tcatgcgatg cactcaaaat gccaatcatc caggatgccc tagcagtact gaccaacgcg 2761 gtgattatcc cccactcagg ctgggaaaat tcgcctcttc aggatgatcg gaaaatacag 2821 ctgcattcat cacaggtgct gcgtaacgcc accgggtgcc taaggaatgt tagttcggcc 2881 ggagaggagg cccgcagaag gatgagagag tgtgatgggc ttacggatgc cttgctgtac 2941 gtgatccagt ctgcgctggg gagcagtgag atcgatagca agaccgttga aaactgtgtg 3001 tgcattttaa ggaacctctc gtaccggctg gcggcagaaa cgtctcaggg acagcacatg 3061 ggcacggacg agctggacgg gctactctgt ggcgaggcca atggcaagga tgctgagagc 3121 tctgggtgct ggggcaagaa gaagaagaaa aagaaatccc aagatcagtg ggatggagta 3181 ggacctcttc cagactgtgc tgaaccacca aaagggatcc agatgctgtg gcacccatca 3241 atagtcaaac cctacctcac actgctctct gagtgctcaa atccagacac gctggaaggg 3301 gcggcaggcg ccctgcagaa cttggctgca gggagctgga agtggtcagt atatatccga 3361 gccgctgtcc gaaaagagaa aggcctgccc atcctcgtgg agctgctccg aatagacaat 3421 gaccgtgtgg tgtgcgcggt ggccactgcg ctgcggaaca tggccttgga cgtcagaaat 3481 aaggagctca tcggcaaata cgccatgcga gacctagtcc acaggcttcc aggagggaac 3541 aacagcaaca acactgcaag caaggccatg tcggatgaca cagtgacagc tgtctgctgc 3601 acactgcacg aagtgattac caagaacatg gagaacgcca aggccttacg ggatgccggt 3661 ggcatcgaga agttggtcgg catctccaaa agcaaaggag ataaacactc tccaaaagtg 3721 gtcaaggctg catctcaggt cctcaacagc atgtggcagt accgagatct gaggagtctc 3781 tacaaaaagg atggatggtc acaataccac tttgtagcct cgtcttcaac catcgagagg 3841 gaccggcaaa ggccctactc ctcctcccgc acgccctcca tctcccctgt gcgcgtgtct 3901 cccaacaacc gctcagcaag tgccccagct tcacctcggg aaatgatcag cctcaaagaa 3961 aggaaaacag actacgagtg caccggcagc aacgccacct accacggagc taaaggcgaa 4021 cacacttcca ggaaagatgc catgacagct caaaacactg gaatttcaac tttgtatagg 4081 aattcttatg gtgcgcccgc tgaagacatc aaacacaacc aggtttcagc acagccagtc 4141 ccacaggagc ccagcagaaa agattacgag acctaccagc catttcagaa ttccacaaga 4201 aattacgatg agtccttctt cgaggaccag gtccaccatc gccctcccgc cagcgagtac 4261 accatgcacc tgggtctcaa gtccaccggc aactacgttg acttctactc agctgcccgt 4321 ccctacagtg aactgaacta tgaaacgagc cactacccgg cctcccccga ctcctgggtg 4381 ctcgagtacc catacgacgt acctgattat gcaacttatc cttatgatgt acctgattat 4441 gctagctatc cttatgatgt cccagactac gcatagtgag ggcccgatat ctcgacaatc 4501 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt 4561 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg 4621 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc 4681 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt 4741 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg 4801 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg 4861 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct 4921 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc 4981 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc 5041 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga aacgggggag 5101 gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa 5161 aagacagaat aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg gttcggtccc 5221 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt 5281 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg 5341 tcggggcggc aggccctgcc atagcagatc tgcgcagctg gggctctagg gggtatcccc 5401 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 5461 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 5521 cgttcgccgg ctttccccgt caagctctaa atcggggcat ccctttaggg ttccgattta 5581 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 5641 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 5701 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 5761 aagggatttt ggggatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 5821 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc 5881 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc 5941 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat 6001 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 6061 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga 6121 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc 6181 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat 6241 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg 6301 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc 6361 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca 6421 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct 6481 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga 6541 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg 6601 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat 6661 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga 6721 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg 6781 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg 6841 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat 6901 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct 6961 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga 7021 cgagttcttc tgagcgggac tctggggttc gcgaaatgac cgaccaagcg acgcccaacc 7081 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg 7141 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg 7201 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 7261 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 7321 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt 7381 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 7441 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 7501 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 7561 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 7621 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 7681 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 7741 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 7801 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 7861 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 7921 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct 7981 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 8041 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 8101 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 8161 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 8221 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 8281 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 8341 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 8401 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 8461 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 8521 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 8581 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 8641 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 8701 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 8761 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 8821 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 8881 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 8941 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 9001 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 9061 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 9121 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 9181 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 9241 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 9301 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 9361 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 9421 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 9481 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg 9541 gatcgggaga tctcccgatc ccctatggtc gactctcagt acaatctgct ctgatgccgc 9601 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag 9661 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag 9721 g //