LOCUS       V046964                 9721 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046964
VERSION     V046964
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..4380
                     /label="CTNND2(NM_001332)"
                     /note="CTNND2(NM_001332)"
                     /gene="CTNND2"
     CDS             4387..4413
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4417..4443
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4447..4473
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     misc_feature    4497..5085
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    5151..5199
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5401..5829
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5843..6172
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6239..7033
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    7209..7330
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(7379..7395)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7403..7419
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7427..7457)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7472..7493
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7781..8369)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8540..9400)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9401..9505)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgtt tgcgaggaag
      721 ccgccgggcg ccgcgccttt gggagctatg cctgttccag accagccttc atcagcctca
      781 gagaagacga gttccctgag ccccggctta aacacctcca acggggatgg ctctgaaaca
      841 gaaaccacct ctgccatcct cgcctcagtc aaagaacagg aattacagtt tgaaaggctg
      901 acccgagagc tggaggctga acggcagatc gtagccagcc agctggagcg atgcaagctc
      961 ggatccgaga ctggcagcat gagcagcatg agttcagcag aagagcagtt tcagtggcag
     1021 tcacaagatg gtcaaaaaga tatcgaagat gagcttacaa caggtctcga gctggtggac
     1081 tcctgtatta ggtcactaca ggaatcagga atacttgacc cacaggatta ttctacaggt
     1141 gaaaggccca gcctgctctc ccagagtgca cttcagctca attccaaacc tgaagggtct
     1201 ttccagtatc cggccagcta ccatagcaac cagaccctgg ccctggggga aaccacccct
     1261 tcacagctcc cggcccgagg cacacaagcc cgagctacgg gccagagctt cagccagggc
     1321 acgaccagcc gcgccggcca cctggcgggg cccgagcccg cgccgccgcc gccgccgccg
     1381 ccgcgggagc cgttcgcgcc cagcctgggc agcgccttcc acctgcccga cgcgccgccc
     1441 gccgccgccg ccgccgcgct ctactactcc agctccacgc tgcccgcgcc gccgcgcggg
     1501 ggctccccgc tggccgcgcc ccagggcggt tcgcccacca agctgcagcg cggcggctcg
     1561 gcccccgagg gcgccaccta cgccgcgccg cgcggctcct cgcccaagca gtcgcccagc
     1621 cgcctggcca agtcctacag caccagctcg cccatcaaca tcgtcgtgtc ctcggccggc
     1681 ctgtccccga tccgcgtgac ctcgcccccc accgtgcagt ccaccatctc ctcctcgccc
     1741 atccaccagc tgagctccac catcggcacg tacgccaccc tgtcgcccac caagcgcctg
     1801 gtccacgcgt ccgagcagta cagcaagcac tcgcaggagc tgtatgccac ggccaccctc
     1861 cagaggccgg gcagcctggc agctggttcc cgagcctcat acagcagcca gcatgggcac
     1921 ctgggcccag agttgcgggc cctgcagtcc ccagaacacc acatagatcc catctatgaa
     1981 gaccgcgtct atcagaagcc ccctatgagg agtctcagcc agagccaggg ggaccctctg
     2041 ccgccagcac acaccggcac ctaccgcacg agcacagccc catcttcccc tggtgtcgac
     2101 tccgtcccct tgcagcgcac aggcagccag cacggcccac agaatgccgc cgcggccacc
     2161 ttccagaggg ccagctatgc cgccggccca gcctccaatt acgcggaccc ctaccgacag
     2221 ctgcagtatt gtccctctgt tgagtctcca tacagcaaat ccggccctgc tctcccgcct
     2281 gaaggcacct tggccaggtc cccgtccatt gatagcattc agaaagatcc cagagaattt
     2341 ggatggagag acccggaact gccggaagtg attcagatgt tgcagcacca gtttccctcg
     2401 gtccagtcta acgcggcagc ctacttgcaa cacctctgtt ttggagacaa caaaattaaa
     2461 gccgagataa ggagacaagg aggcatccag ctcctggtgg acctgttgga tcatcggatg
     2521 accgaagtcc accgtagtgc ctgtggagct ctgagaaacc tggtgtatgg gaaggccaac
     2581 gatgataaca aaattgccct gaaaaactgt ggtggcatcc cagcactggt gaggttactc
     2641 cgcaagacga ctgacctgga gatccgggag ctggtcacag gagtcctttg gaacctctcc
     2701 tcatgcgatg cactcaaaat gccaatcatc caggatgccc tagcagtact gaccaacgcg
     2761 gtgattatcc cccactcagg ctgggaaaat tcgcctcttc aggatgatcg gaaaatacag
     2821 ctgcattcat cacaggtgct gcgtaacgcc accgggtgcc taaggaatgt tagttcggcc
     2881 ggagaggagg cccgcagaag gatgagagag tgtgatgggc ttacggatgc cttgctgtac
     2941 gtgatccagt ctgcgctggg gagcagtgag atcgatagca agaccgttga aaactgtgtg
     3001 tgcattttaa ggaacctctc gtaccggctg gcggcagaaa cgtctcaggg acagcacatg
     3061 ggcacggacg agctggacgg gctactctgt ggcgaggcca atggcaagga tgctgagagc
     3121 tctgggtgct ggggcaagaa gaagaagaaa aagaaatccc aagatcagtg ggatggagta
     3181 ggacctcttc cagactgtgc tgaaccacca aaagggatcc agatgctgtg gcacccatca
     3241 atagtcaaac cctacctcac actgctctct gagtgctcaa atccagacac gctggaaggg
     3301 gcggcaggcg ccctgcagaa cttggctgca gggagctgga agtggtcagt atatatccga
     3361 gccgctgtcc gaaaagagaa aggcctgccc atcctcgtgg agctgctccg aatagacaat
     3421 gaccgtgtgg tgtgcgcggt ggccactgcg ctgcggaaca tggccttgga cgtcagaaat
     3481 aaggagctca tcggcaaata cgccatgcga gacctagtcc acaggcttcc aggagggaac
     3541 aacagcaaca acactgcaag caaggccatg tcggatgaca cagtgacagc tgtctgctgc
     3601 acactgcacg aagtgattac caagaacatg gagaacgcca aggccttacg ggatgccggt
     3661 ggcatcgaga agttggtcgg catctccaaa agcaaaggag ataaacactc tccaaaagtg
     3721 gtcaaggctg catctcaggt cctcaacagc atgtggcagt accgagatct gaggagtctc
     3781 tacaaaaagg atggatggtc acaataccac tttgtagcct cgtcttcaac catcgagagg
     3841 gaccggcaaa ggccctactc ctcctcccgc acgccctcca tctcccctgt gcgcgtgtct
     3901 cccaacaacc gctcagcaag tgccccagct tcacctcggg aaatgatcag cctcaaagaa
     3961 aggaaaacag actacgagtg caccggcagc aacgccacct accacggagc taaaggcgaa
     4021 cacacttcca ggaaagatgc catgacagct caaaacactg gaatttcaac tttgtatagg
     4081 aattcttatg gtgcgcccgc tgaagacatc aaacacaacc aggtttcagc acagccagtc
     4141 ccacaggagc ccagcagaaa agattacgag acctaccagc catttcagaa ttccacaaga
     4201 aattacgatg agtccttctt cgaggaccag gtccaccatc gccctcccgc cagcgagtac
     4261 accatgcacc tgggtctcaa gtccaccggc aactacgttg acttctactc agctgcccgt
     4321 ccctacagtg aactgaacta tgaaacgagc cactacccgg cctcccccga ctcctgggtg
     4381 ctcgagtacc catacgacgt acctgattat gcaacttatc cttatgatgt acctgattat
     4441 gctagctatc cttatgatgt cccagactac gcatagtgag ggcccgatat ctcgacaatc
     4501 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     4561 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     4621 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     4681 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     4741 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     4801 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     4861 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     4921 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     4981 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     5041 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga aacgggggag
     5101 gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa
     5161 aagacagaat aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg gttcggtccc
     5221 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt
     5281 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg
     5341 tcggggcggc aggccctgcc atagcagatc tgcgcagctg gggctctagg gggtatcccc
     5401 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     5461 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     5521 cgttcgccgg ctttccccgt caagctctaa atcggggcat ccctttaggg ttccgattta
     5581 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     5641 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     5701 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     5761 aagggatttt ggggatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     5821 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     5881 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     5941 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     6001 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     6061 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     6121 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     6181 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat
     6241 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     6301 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     6361 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     6421 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     6481 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     6541 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     6601 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     6661 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     6721 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
     6781 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     6841 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     6901 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     6961 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     7021 cgagttcttc tgagcgggac tctggggttc gcgaaatgac cgaccaagcg acgcccaacc
     7081 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     7141 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     7201 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     7261 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     7321 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     7381 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     7441 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     7501 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     7561 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     7621 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     7681 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     7741 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     7801 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     7861 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     7921 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct
     7981 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     8041 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     8101 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     8161 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     8221 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     8281 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     8341 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     8401 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     8461 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     8521 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     8581 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     8641 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     8701 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     8761 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     8821 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     8881 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     8941 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     9001 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     9061 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     9121 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     9181 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     9241 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     9301 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     9361 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     9421 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     9481 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
     9541 gatcgggaga tctcccgatc ccctatggtc gactctcagt acaatctgct ctgatgccgc
     9601 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
     9661 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
     9721 g
//
