LOCUS       V030207                 6252 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030207
VERSION     V030207
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2399
                     /label="Cd4(NM_013488)"
                     /note="Cd4(NM_013488)"
                     /gene="Cd4"
     CDS             2418..2447
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2466..2489
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2509..2525)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2536..3158
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3307..3895)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4224..4271
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4503..5297)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5332..5689)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5691..5795)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5822..6250
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gtgccgagcc atctctctta ggcgcttgct gctgctgctg ctgcagctgt
     1081 cacaactcct agctgtcact caagggaaga cgctggtgct ggggaaggaa ggggaatcag
     1141 cagaactgcc ctgcgagagt tcccagaaga agatcacagt cttcacctgg aagttctctg
     1201 accagaggaa gattctgggg cagcatggca aaggtgtatt aattagagga ggttcgcctt
     1261 cgcagtttga tcgttttgat tccaaaaaag gggcatggga gaaaggatcg tttcctctca
     1321 tcatcaataa acttaagatg gaagactctc agacttatat ctgtgagctg gagaacagga
     1381 aagaggaggt ggagttgtgg gtgttcaaag tgaccttcag tccgggtacc agcctgttgc
     1441 aagggcagag cctgaccctg accttggata gcaactctaa ggtctctaac cccttgacag
     1501 agtgcaaaca caaaaagggt aaagttgtca gtggttccaa agttctctcc atgtccaacc
     1561 taagggttca ggacagcgac ttctggaact gcaccgtgac cctggaccag aaaaagaact
     1621 ggttcggcat gacactctca gtgctgggtt ttcagagcac agctatcacg gcctataaga
     1681 gtgagggaga gtcagcggag ttctccttcc cactcaactt tgcagaggaa aacgggtggg
     1741 gagagctgat gtggaaggca gagaaggatt ctttcttcca gccctggatc tccttctcca
     1801 taaagaacaa agaggtgtcc gtacaaaagt ccaccaaaga cctcaagctc cagctgaagg
     1861 aaacgctccc actcaccctc aagatacccc aggtctcgct tcagtttgct ggttctggca
     1921 acctgactct gactctggac aaagggacac tgcatcagga agtgaacctg gtggtgatga
     1981 aagtggctca gctcaacaat actttgacct gtgaggtgat gggacctacc tctcccaaga
     2041 tgagactgac cctgaagcag gagaaccagg aggccagggt ctctgaggag cagaaagtag
     2101 ttcaagtggt ggcccctgag acagggctgt ggcagtgtct actgagtgaa ggtgataagg
     2161 tcaagatgga ctccaggatc caggttttat ccagaggggt gaaccagaca gtgttcctgg
     2221 cttgcgtgct gggtggctcc ttcggctttc tgggtttcct tgggctctgc atcctctgct
     2281 gtgtcaggtg ccggcaccaa cagcgccagg cagcacgaat gtctcagatc aagaggctcc
     2341 tcagtgagaa gaagacctgc cagtgccccc accggatgca gaagagccat aatctcatca
     2401 cgcgtacgcg gccgctcgag cagaaactca tctcagaaga ggatctggca gcaaatgata
     2461 tcctggatta caaggatgac gacgataagg tttaaacggc cggccgcggt catagctgtt
     2521 tcctgaacag atcccgggtg gcatccctgt gacccctccc cagtgcctct cctggccctg
     2581 gaagttgcca ctccagtgcc caccagcctt gtcctaataa aattaagttg catcattttg
     2641 tctgactagg tgtccttcta taatattatg gggtggaggg gggtggtatg gagcaagggg
     2701 caagttggga agacaacctg tagggcctgc ggggtctatt gggaaccaag ctggagtgca
     2761 gtggcacaat cttggctcac tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct
     2821 cagcctcccg agttgttggg attccaggca tgcatgacca ggctcagcta atttttgttt
     2881 ttttggtaga gacggggttt caccatattg gccaggctgg tctccaactc ctaatctcag
     2941 gtgatctacc caccttggcc tcccaaattg ctgggattac aggcgtgaac cactgctccc
     3001 ttccctgtcc ttctgatttt aaaataacta taccagcagg aggacgtcca gacacagcat
     3061 aggctacctg gccatgccca accggtggga catttgagtt gcttgcttgg cactgtcctc
     3121 tcatgcgttg ggtccactca gtagatgcct gttgaattgg gtacgcggcc agcggcgagc
     3181 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     3241 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     3301 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     3361 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     3421 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     3481 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     3541 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     3601 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     3661 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     3721 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     3781 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     3841 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     3901 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     3961 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     4021 ttttaaatca atctaaagta tatatgagta acctgaggct atggcagggc ctgccgcccc
     4081 gacgttggct gcgagccctg ggccttcacc cgaacttggg gggtggggtg gggaaaagga
     4141 agaaacgcgg gcgtattggc cccaatgggg tctcggtggg gtatcgacag agtgccagcc
     4201 ctgggaccga accccgcgtt tatgaacaaa cgacccaaca ccgtgcgttt tattctgtct
     4261 ttttattgcc gtcatagcgc gggttccttc cggtattgtc tccttccgtg tttcagttag
     4321 cctcccccta gggtgggcga agaactccag catgagatcc ccgcgctgga ggatcatcca
     4381 gccggcgtcc cggaaaacga ttccgaagcc caacctttca tagaaggcgg cggtggaatc
     4441 gaaatctcgt gatggcaggt tgggcgtcgc ttggtcggtc atttcgaacc ccagagtccc
     4501 gctcagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg
     4561 ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca
     4621 cgggtagcca acgctatgtc ctgatagcga tccgccacac ccagccggcc acagtcgatg
     4681 aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc gccatgggtc
     4741 acgacgagat cctcgccgtc gggcatgctc gccttgagcc tggcgaacag ttcggctggc
     4801 gcgagcccct gatgctcttc gtccagatca tcctgatcga caagaccggc ttccatccga
     4861 gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca
     4921 agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg
     4981 tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct
     5041 tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc
     5101 cgcgctgcct cgtcttgcag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga
     5161 accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt
     5221 tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat
     5281 ccatcttgtt caatcatgcg aaacgatcct catcctgtct cttgatcgat ctttgcaaaa
     5341 gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc
     5401 ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact
     5461 gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt
     5521 gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc cacacctggt
     5581 tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt
     5641 tccacaccct aactgacaca cattccacag ctggttcttt ccgcctcagg actcttcctt
     5701 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     5761 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     5821 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     5881 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     5941 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     6001 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     6061 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     6121 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     6181 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     6241 aacgcgaatt tt
//
