LOCUS       V031326                 6699 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031326
VERSION     V031326
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2846
                     /label="Cry1(NM_007771)"
                     /note="Cry1(NM_007771)"
                     /gene="Cry1"
     CDS             2865..2894
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2913..2936
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2956..2972)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2983..3605
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3754..4342)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4671..4718
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4950..5744)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5779..6136)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(6138..6242)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      6269..6697
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gggggtgaac gccgtgcact ggttccgaaa gggactccgg ctccacgaca
     1081 accccgccct gaaggagtgc atccagggcg ccgacaccat ccgctgcgtc tatatcctcg
     1141 acccctggtt cgccggctct tccaacgtgg gcatcaacag gtggcgattt ttgcttcagt
     1201 gtcttgagga tcttgatgcc aatctacgaa aattaaattc tcgtctgttt gtgattcggg
     1261 gacagccagc tgatgtattt cccaggcttt tcaaggaatg gaacatcact aaactctcaa
     1321 ttgagtatga ttctgagcct tttgggaagg aacgagatgc agctatcaag aagctggcta
     1381 ctgaggctgg cgtggaagtc atcgtgcgca tttcacatac actgtatgac ctggacaaga
     1441 tcatagaact caatggcgga cagccacctc taacatataa aaggtttcag actctcgtca
     1501 gcaagatgga gccactggag atgccagcag acaccatcac atcagatgtg ataggaaagt
     1561 gcatgacccc tctgtctgat gaccatgatg agaaatatgg cgttccttcc ctggaagagc
     1621 tcggctttga tacagatggc ctgtcctctg cagtgtggcc aggaggagaa actgaggcac
     1681 ttacacgttt ggaaaggcat ttggaaagaa aggcctgggt ggcaaacttt gaacgacctc
     1741 gaatgaatgc aaactccctg cttgcaagcc caactggact cagtccttat ctccgctttg
     1801 gttgtttatc atgtcggctg ttttatttca aactaacaga tctctacaaa aaggtaaaga
     1861 agaatagttc ccctcccctt tctctttatg ggcaactcct gtggcgtgaa tttttttata
     1921 cagcagccac aaacaaccca cgctttgaca aaatggaagg gaaccccatc tgtgttcaga
     1981 tcccttggga caagaacccc gaggctctgg ccaaatgggc agaaggccgg acaggcttcc
     2041 cgtggattga cgccatcatg actcagcttc gtcaggaggg ctggatccac catttagcca
     2101 gacacgcggt tgcctgtttc ctgactcgtg gtgacctgtg gatcagctgg gaagaaggga
     2161 tgaaggtctt tgaagagtta ctgcttgatg cagattggag cataaatgct ggaagttgga
     2221 tgtggctgtc ctgcagttcc ttttttcagc aattttttca ctgctactgc cctgtgggtt
     2281 ttggtaggag gacagatccc aatggagact atattaggcg ttatttacct gtcctaagag
     2341 gcttccctgc aaaatatatc tacgatcctt ggaatgcacc agaaggcatc cagaaggttg
     2401 ccaagtgttt gataggagtt aattacccca aaccgatggt gaaccatgct gaggcaagca
     2461 gactgaatat tgaaagaatg aagcagatct atcagcagct ttcccggtac agagggctag
     2521 gtcttctcgc ctcggtccct tctaactcta atgggaatgg agggctcatg ggctatgctc
     2581 ctggagagaa tgtcccgagt tgtagcagca gcgggaatgg agggctcatg ggctatgctc
     2641 ctggagagaa cgtcccgagc tgtagcggtg gaaattgctc tcaaggaagt ggtattttac
     2701 actatgctca cggggacagt cagcagactc actcactcaa gcaagggaga agctccgcgg
     2761 gcaccggcct cagcagtggg aagcgtccta gtcaggaaga ggatgcccag agtgtcggcc
     2821 ccaaagtcca gcggcagagc agtaacacgc gtacgcggcc gctcgagcag aaactcatct
     2881 cagaagagga tctggcagca aatgatatcc tggattacaa ggatgacgac gataaggttt
     2941 aaacggccgg ccgcggtcat agctgtttcc tgaacagatc ccgggtggca tccctgtgac
     3001 ccctccccag tgcctctcct ggccctggaa gttgccactc cagtgcccac cagccttgtc
     3061 ctaataaaat taagttgcat cattttgtct gactaggtgt ccttctataa tattatgggg
     3121 tggagggggg tggtatggag caaggggcaa gttgggaaga caacctgtag ggcctgcggg
     3181 gtctattggg aaccaagctg gagtgcagtg gcacaatctt ggctcactgc aatctccgcc
     3241 tcctgggttc aagcgattct cctgcctcag cctcccgagt tgttgggatt ccaggcatgc
     3301 atgaccaggc tcagctaatt tttgtttttt tggtagagac ggggtttcac catattggcc
     3361 aggctggtct ccaactccta atctcaggtg atctacccac cttggcctcc caaattgctg
     3421 ggattacagg cgtgaaccac tgctcccttc cctgtccttc tgattttaaa ataactatac
     3481 cagcaggagg acgtccagac acagcatagg ctacctggcc atgcccaacc ggtgggacat
     3541 ttgagttgct tgcttggcac tgtcctctca tgcgttgggt ccactcagta gatgcctgtt
     3601 gaattgggta cgcggccagc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     3661 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     3721 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     3781 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     3841 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     3901 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     3961 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     4021 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     4081 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     4141 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     4201 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     4261 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     4321 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     4381 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     4441 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaacc
     4501 tgaggctatg gcagggcctg ccgccccgac gttggctgcg agccctgggc cttcacccga
     4561 acttgggggg tggggtgggg aaaaggaaga aacgcgggcg tattggcccc aatggggtct
     4621 cggtggggta tcgacagagt gccagccctg ggaccgaacc ccgcgtttat gaacaaacga
     4681 cccaacaccg tgcgttttat tctgtctttt tattgccgtc atagcgcggg ttccttccgg
     4741 tattgtctcc ttccgtgttt cagttagcct ccccctaggg tgggcgaaga actccagcat
     4801 gagatccccg cgctggagga tcatccagcc ggcgtcccgg aaaacgattc cgaagcccaa
     4861 cctttcatag aaggcggcgg tggaatcgaa atctcgtgat ggcaggttgg gcgtcgcttg
     4921 gtcggtcatt tcgaacccca gagtcccgct cagaagaact cgtcaagaag gcgatagaag
     4981 gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat
     5041 tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcgatcc
     5101 gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata
     5161 ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catgctcgcc
     5221 ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc
     5281 tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg
     5341 tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg
     5401 atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg
     5461 cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga
     5521 acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cttgcagttc attcagggca
     5581 ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg
     5641 gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc
     5701 caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat
     5761 cctgtctctt gatcgatctt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt
     5821 ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca
     5881 gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag
     5941 gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
     6001 ggagcctggg gactttccac acctggttgc tgactaattg agatgcatgc tttgcatact
     6061 tctgcctgct ggggagcctg gggactttcc acaccctaac tgacacacat tccacagctg
     6121 gttctttccg cctcaggact cttccttttt caatattatt gaagcattta tcagggttat
     6181 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     6241 cgcacatttc cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg
     6301 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     6361 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     6421 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     6481 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     6541 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     6601 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     6661 aaaaatgagc tgatttaaca aaaatttaac gcgaatttt
//
