LOCUS V030628 5208 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V030628 VERSION V030628 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers primer_bind 166..182 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 343..722 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 723..926 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 952..970 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 1029..1355 /label="Cxcl13(NM_018866)" /note="Cxcl13(NM_018866)" /gene="Cxcl13" CDS 1374..1403 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" CDS 1422..1445 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" primer_bind complement(1465..1481) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" polyA_signal 1492..2114 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" rep_origin complement(2263..2851) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" polyA_signal 3180..3227 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" CDS complement(3459..4253) /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" promoter complement(4288..4645) /note="SV40 enhancer and early promoter" /label="SV40 promoter" promoter complement(4647..4751) /gene="bla" /label="AmpR promoter" rep_origin 4778..5206 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" ORIGIN 1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag 61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt 241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata 301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta 361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat 781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac 901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac 961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg 1021 cgatcgccat gaggctcagc acagcaacgc tgcttctcct cctggccagc tgcctctctc 1081 caggccacgg tattctggaa gcccattaca caaacttaaa atgtaggtgt tctggagtga 1141 tttcaactgt tgtcggtcta aacatcatag atcggattca agttacgccc cctgggaatg 1201 gctgccccaa aactgaagtt gtgatctgga ccaagatgaa gaaagttata tgtgtgaatc 1261 ctcgtgccaa atggttacaa agattattaa gacatgtcca aagcaaaagt ctgtcttcaa 1321 ctccccaagc tccagtgagt aagagaagag ctgccacgcg tacgcggccg ctcgagcaga 1381 aactcatctc agaagaggat ctggcagcaa atgatatcct ggattacaag gatgacgacg 1441 ataaggttta aacggccggc cgcggtcata gctgtttcct gaacagatcc cgggtggcat 1501 ccctgtgacc cctccccagt gcctctcctg gccctggaag ttgccactcc agtgcccacc 1561 agccttgtcc taataaaatt aagttgcatc attttgtctg actaggtgtc cttctataat 1621 attatggggt ggaggggggt ggtatggagc aaggggcaag ttgggaagac aacctgtagg 1681 gcctgcgggg tctattggga accaagctgg agtgcagtgg cacaatcttg gctcactgca 1741 atctccgcct cctgggttca agcgattctc ctgcctcagc ctcccgagtt gttgggattc 1801 caggcatgca tgaccaggct cagctaattt ttgttttttt ggtagagacg gggtttcacc 1861 atattggcca ggctggtctc caactcctaa tctcaggtga tctacccacc ttggcctccc 1921 aaattgctgg gattacaggc gtgaaccact gctcccttcc ctgtccttct gattttaaaa 1981 taactatacc agcaggagga cgtccagaca cagcataggc tacctggcca tgcccaaccg 2041 gtgggacatt tgagttgctt gcttggcact gtcctctcat gcgttgggtc cactcagtag 2101 atgcctgttg aattgggtac gcggccagcg gcgagcggta tcagctcact caaaggcggt 2161 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 2221 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 2281 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 2341 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 2401 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 2461 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 2521 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 2581 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 2641 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 2701 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 2761 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 2821 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 2881 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 2941 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 3001 tgagtaacct gaggctatgg cagggcctgc cgccccgacg ttggctgcga gccctgggcc 3061 ttcacccgaa cttggggggt ggggtgggga aaaggaagaa acgcgggcgt attggcccca 3121 atggggtctc ggtggggtat cgacagagtg ccagccctgg gaccgaaccc cgcgtttatg 3181 aacaaacgac ccaacaccgt gcgttttatt ctgtcttttt attgccgtca tagcgcgggt 3241 tccttccggt attgtctcct tccgtgtttc agttagcctc cccctagggt gggcgaagaa 3301 ctccagcatg agatccccgc gctggaggat catccagccg gcgtcccgga aaacgattcc 3361 gaagcccaac ctttcataga aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg 3421 cgtcgcttgg tcggtcattt cgaaccccag agtcccgctc agaagaactc gtcaagaagg 3481 cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg 3541 tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga 3601 tagcgatccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc 3661 accatgatat tcggcaagca ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc 3721 atgctcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc 3781 agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt 3841 ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca 3901 tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc 3961 ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct 4021 gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ttgcagttca 4081 ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc 4141 cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc 4201 ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcgaaac 4261 gatcctcatc ctgtctcttg atcgatcttt gcaaaagcct aggcctccaa aaaagcctcc 4321 tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa 4381 aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg 4441 cggagttagg ggcgggacta tggttgctga ctaattgaga tgcatgcttt gcatacttct 4501 gcctgctggg gagcctgggg actttccaca cctggttgct gactaattga gatgcatgct 4561 ttgcatactt ctgcctgctg gggagcctgg ggactttcca caccctaact gacacacatt 4621 ccacagctgg ttctttccgc ctcaggactc ttcctttttc aatattattg aagcatttat 4681 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 4741 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta 4801 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg 4861 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa 4921 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc 4981 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt 5041 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca 5101 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc 5161 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaatttt //