LOCUS       V030628                 5208 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030628
VERSION     V030628
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..1355
                     /label="Cxcl13(NM_018866)"
                     /note="Cxcl13(NM_018866)"
                     /gene="Cxcl13"
     CDS             1374..1403
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             1422..1445
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(1465..1481)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    1492..2114
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(2263..2851)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    3180..3227
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(3459..4253)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(4288..4645)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(4647..4751)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      4778..5206
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gaggctcagc acagcaacgc tgcttctcct cctggccagc tgcctctctc
     1081 caggccacgg tattctggaa gcccattaca caaacttaaa atgtaggtgt tctggagtga
     1141 tttcaactgt tgtcggtcta aacatcatag atcggattca agttacgccc cctgggaatg
     1201 gctgccccaa aactgaagtt gtgatctgga ccaagatgaa gaaagttata tgtgtgaatc
     1261 ctcgtgccaa atggttacaa agattattaa gacatgtcca aagcaaaagt ctgtcttcaa
     1321 ctccccaagc tccagtgagt aagagaagag ctgccacgcg tacgcggccg ctcgagcaga
     1381 aactcatctc agaagaggat ctggcagcaa atgatatcct ggattacaag gatgacgacg
     1441 ataaggttta aacggccggc cgcggtcata gctgtttcct gaacagatcc cgggtggcat
     1501 ccctgtgacc cctccccagt gcctctcctg gccctggaag ttgccactcc agtgcccacc
     1561 agccttgtcc taataaaatt aagttgcatc attttgtctg actaggtgtc cttctataat
     1621 attatggggt ggaggggggt ggtatggagc aaggggcaag ttgggaagac aacctgtagg
     1681 gcctgcgggg tctattggga accaagctgg agtgcagtgg cacaatcttg gctcactgca
     1741 atctccgcct cctgggttca agcgattctc ctgcctcagc ctcccgagtt gttgggattc
     1801 caggcatgca tgaccaggct cagctaattt ttgttttttt ggtagagacg gggtttcacc
     1861 atattggcca ggctggtctc caactcctaa tctcaggtga tctacccacc ttggcctccc
     1921 aaattgctgg gattacaggc gtgaaccact gctcccttcc ctgtccttct gattttaaaa
     1981 taactatacc agcaggagga cgtccagaca cagcataggc tacctggcca tgcccaaccg
     2041 gtgggacatt tgagttgctt gcttggcact gtcctctcat gcgttgggtc cactcagtag
     2101 atgcctgttg aattgggtac gcggccagcg gcgagcggta tcagctcact caaaggcggt
     2161 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     2221 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     2281 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     2341 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     2401 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     2461 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     2521 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     2581 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     2641 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     2701 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     2761 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     2821 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     2881 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     2941 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     3001 tgagtaacct gaggctatgg cagggcctgc cgccccgacg ttggctgcga gccctgggcc
     3061 ttcacccgaa cttggggggt ggggtgggga aaaggaagaa acgcgggcgt attggcccca
     3121 atggggtctc ggtggggtat cgacagagtg ccagccctgg gaccgaaccc cgcgtttatg
     3181 aacaaacgac ccaacaccgt gcgttttatt ctgtcttttt attgccgtca tagcgcgggt
     3241 tccttccggt attgtctcct tccgtgtttc agttagcctc cccctagggt gggcgaagaa
     3301 ctccagcatg agatccccgc gctggaggat catccagccg gcgtcccgga aaacgattcc
     3361 gaagcccaac ctttcataga aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg
     3421 cgtcgcttgg tcggtcattt cgaaccccag agtcccgctc agaagaactc gtcaagaagg
     3481 cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg
     3541 tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga
     3601 tagcgatccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc
     3661 accatgatat tcggcaagca ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc
     3721 atgctcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc
     3781 agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt
     3841 ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca
     3901 tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc
     3961 ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct
     4021 gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ttgcagttca
     4081 ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc
     4141 cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc
     4201 ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcgaaac
     4261 gatcctcatc ctgtctcttg atcgatcttt gcaaaagcct aggcctccaa aaaagcctcc
     4321 tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa
     4381 aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg
     4441 cggagttagg ggcgggacta tggttgctga ctaattgaga tgcatgcttt gcatacttct
     4501 gcctgctggg gagcctgggg actttccaca cctggttgct gactaattga gatgcatgct
     4561 ttgcatactt ctgcctgctg gggagcctgg ggactttcca caccctaact gacacacatt
     4621 ccacagctgg ttctttccgc ctcaggactc ttcctttttc aatattattg aagcatttat
     4681 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     4741 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta
     4801 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     4861 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     4921 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     4981 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     5041 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     5101 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     5161 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaatttt
//
