LOCUS       V031164                 6819 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031164
VERSION     V031164
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2966
                     /label="Ddx43(NM_001191044)"
                     /note="Ddx43(NM_001191044)"
                     /gene="Ddx43"
     CDS             2985..3014
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             3033..3056
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(3076..3092)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    3103..3725
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3874..4462)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4791..4838
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(5070..5864)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5899..6256)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(6258..6362)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      6389..6817
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gtctaggcgg gaacccgcag ccaacgcttc ctcctcgggc gttgctcccc
     1081 ggcgaagctc gaccgggtcc cggggcgccg accggacagc gacggaggag acgaaccgga
     1141 gaggccgggt cggtagcagg ggtgcgggcg gcggctggag agagcccgcc ggggccgccg
     1201 aggctacggc cgccggccgc cgcgaaccgc cgctctgctt cggggtgaag aacgatgtgg
     1261 tgggcgcggt gataggtcgt ggtgggtcaa aaattagaga aatccaaaat acaacgaaca
     1321 ctaggataca ggtaataaaa ggaaatcctg aggcagaaat caaaattttt ggtaataaag
     1381 ccatgcaaac aaaagcaaaa acagtgatag ataatgttgt taaaaaacaa caaaattaca
     1441 ttccaggaca aagagttggt attattgcat tccaacctac cgttggagca gataccagca
     1501 caagtgagag tgtaacagac gatcagccat tgatagattg ggatcaaatt cgagaagatg
     1561 ctttgaaatg ggaaaagaaa aagtgggcag atttacctcc aattaagaaa aacttttaca
     1621 tagagtcagc aacgacaagc tcaatgtcac aagtgcaaat agacaactgg aggaaagaaa
     1681 attttaatat aacatgtgat gacttgaaag atggtgagaa gcgtcctatc cccaacccta
     1741 tatgtaaatt tgaagatgcc tttcaaagtt acccagaagt aatggaaaac attaaaagag
     1801 caggctttca aaaacctaca ccgattcagt cacaggcatg gccaatagtt ctacaaggaa
     1861 tagatcttat tggagtagca caaactggaa cagggaagac actgtcctat ttaatgcctg
     1921 gatttattca tctagactct caaccactag ctagagaaca aagaaatggg cctggcatgt
     1981 tagttcttac gcccacccgg gaattagccc ttcaagtaga agctgaatgt tctaaatatt
     2041 catatggtga tcttaaaagt gtttgtgtat atggtggtgg tgatagagat ggacaaatac
     2101 aagatgtttc aaaaggtgta gacatcatta ttgcaactcc tggaagattg aatgacctac
     2161 aaatgaataa ctttgtcaat ctgaaaagtg taacctattt ggttttagat gaagcagaca
     2221 agatgctgga catgggattt gaaccccaga taatgaagat tttgttagat gtacgcccag
     2281 accggcagac tattatgaca agcgctactt ggccatatgc cgtccgccgt cttgcacaat
     2341 cttatttgaa agaaccaatg attgtctatg ttggtacttt ggatctagtt gctgtaagta
     2401 cagtgaaaca aaatataatc attaccacag aagaagagaa acgaactcat atccagacct
     2461 tcctcgagaa tatgtcacct aaagacaaag tcattgtctt tgtcagcaga aaagccgttg
     2521 ctgatcactt atcaagtgat ctgattctcc gacacatatc agtggagtct ctgcacggca
     2581 acagagaaca gagtgaccga gagaaagcgt tagagaactt taagacaggt aaagtgagaa
     2641 tacttattgc tactgacttg gcatctcgag gtcttgatgt ccatgatatc actcatgtct
     2701 acaattatga ttttccacgg aacattgaag aatatgtaca cagagtaggg cgcactggga
     2761 gagcagggcg cactggaatg tcaattaccc ttatcacaag aaatgattgg agggttgcca
     2821 ctgaattgat taatattcta gaaagagcaa atcagaatat cccagaggaa cttgtcttaa
     2881 tggctgagag atacaaagca aataaactaa aaagagagat ggaaaaaaaa atgggaagac
     2941 ctcaaggaaa gccccagaag ttttacacgc gtacgcggcc gctcgagcag aaactcatct
     3001 cagaagagga tctggcagca aatgatatcc tggattacaa ggatgacgac gataaggttt
     3061 aaacggccgg ccgcggtcat agctgtttcc tgaacagatc ccgggtggca tccctgtgac
     3121 ccctccccag tgcctctcct ggccctggaa gttgccactc cagtgcccac cagccttgtc
     3181 ctaataaaat taagttgcat cattttgtct gactaggtgt ccttctataa tattatgggg
     3241 tggagggggg tggtatggag caaggggcaa gttgggaaga caacctgtag ggcctgcggg
     3301 gtctattggg aaccaagctg gagtgcagtg gcacaatctt ggctcactgc aatctccgcc
     3361 tcctgggttc aagcgattct cctgcctcag cctcccgagt tgttgggatt ccaggcatgc
     3421 atgaccaggc tcagctaatt tttgtttttt tggtagagac ggggtttcac catattggcc
     3481 aggctggtct ccaactccta atctcaggtg atctacccac cttggcctcc caaattgctg
     3541 ggattacagg cgtgaaccac tgctcccttc cctgtccttc tgattttaaa ataactatac
     3601 cagcaggagg acgtccagac acagcatagg ctacctggcc atgcccaacc ggtgggacat
     3661 ttgagttgct tgcttggcac tgtcctctca tgcgttgggt ccactcagta gatgcctgtt
     3721 gaattgggta cgcggccagc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     3781 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     3841 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     3901 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     3961 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     4021 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     4081 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     4141 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     4201 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     4261 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     4321 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     4381 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     4441 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     4501 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     4561 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaacc
     4621 tgaggctatg gcagggcctg ccgccccgac gttggctgcg agccctgggc cttcacccga
     4681 acttgggggg tggggtgggg aaaaggaaga aacgcgggcg tattggcccc aatggggtct
     4741 cggtggggta tcgacagagt gccagccctg ggaccgaacc ccgcgtttat gaacaaacga
     4801 cccaacaccg tgcgttttat tctgtctttt tattgccgtc atagcgcggg ttccttccgg
     4861 tattgtctcc ttccgtgttt cagttagcct ccccctaggg tgggcgaaga actccagcat
     4921 gagatccccg cgctggagga tcatccagcc ggcgtcccgg aaaacgattc cgaagcccaa
     4981 cctttcatag aaggcggcgg tggaatcgaa atctcgtgat ggcaggttgg gcgtcgcttg
     5041 gtcggtcatt tcgaacccca gagtcccgct cagaagaact cgtcaagaag gcgatagaag
     5101 gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat
     5161 tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcgatcc
     5221 gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata
     5281 ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catgctcgcc
     5341 ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc
     5401 tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg
     5461 tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg
     5521 atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg
     5581 cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga
     5641 acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cttgcagttc attcagggca
     5701 ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg
     5761 gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc
     5821 caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat
     5881 cctgtctctt gatcgatctt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt
     5941 ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca
     6001 gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag
     6061 gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
     6121 ggagcctggg gactttccac acctggttgc tgactaattg agatgcatgc tttgcatact
     6181 tctgcctgct ggggagcctg gggactttcc acaccctaac tgacacacat tccacagctg
     6241 gttctttccg cctcaggact cttccttttt caatattatt gaagcattta tcagggttat
     6301 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     6361 cgcacatttc cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg
     6421 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     6481 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     6541 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     6601 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     6661 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     6721 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     6781 aaaaatgagc tgatttaaca aaaatttaac gcgaatttt
//
