LOCUS V029849 5067 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V029849 VERSION V029849 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers primer_bind 166..182 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" enhancer 343..722 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 723..926 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 952..970 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 1029..1214 /label="Defb33(NM_001039119)" /note="Defb33(NM_001039119)" /gene="Defb33" CDS 1233..1262 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" CDS 1281..1304 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" primer_bind complement(1324..1340) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" polyA_signal 1351..1973 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" rep_origin complement(2122..2710) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" polyA_signal 3039..3086 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" CDS complement(3318..4112) /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" promoter complement(4147..4504) /note="SV40 enhancer and early promoter" /label="SV40 promoter" promoter complement(4506..4610) /gene="bla" /label="AmpR promoter" rep_origin 4637..5065 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" ORIGIN 1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag 61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt 241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata 301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta 361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat 781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac 901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac 961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg 1021 cgatcgccat gaggctgtta tttcttctct tcatcctcct tgtgtgtctt gctcagacta 1081 catcagggcg caaaagaaac tcaaagtttc gtccgtgtga gaaaatgggt ggcatttgca 1141 agtctcagaa aactcacgga tgctctatcc tgcctgcaga atgcaagagt cgatacaaac 1201 actgctgtag gttgacgcgt acgcggccgc tcgagcagaa actcatctca gaagaggatc 1261 tggcagcaaa tgatatcctg gattacaagg atgacgacga taaggtttaa acggccggcc 1321 gcggtcatag ctgtttcctg aacagatccc gggtggcatc cctgtgaccc ctccccagtg 1381 cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct aataaaatta 1441 agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg gaggggggtg 1501 gtatggagca aggggcaagt tgggaagaca acctgtaggg cctgcggggt ctattgggaa 1561 ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa 1621 gcgattctcc tgcctcagcc tcccgagttg ttgggattcc aggcatgcat gaccaggctc 1681 agctaatttt tgtttttttg gtagagacgg ggtttcacca tattggccag gctggtctcc 1741 aactcctaat ctcaggtgat ctacccacct tggcctccca aattgctggg attacaggcg 1801 tgaaccactg ctcccttccc tgtccttctg attttaaaat aactatacca gcaggaggac 1861 gtccagacac agcataggct acctggccat gcccaaccgg tgggacattt gagttgcttg 1921 cttggcactg tcctctcatg cgttgggtcc actcagtaga tgcctgttga attgggtacg 1981 cggccagcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 2041 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 2101 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 2161 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 2221 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 2281 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 2341 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 2401 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 2461 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 2521 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg 2581 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 2641 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 2701 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 2761 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 2821 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaacctg aggctatggc 2881 agggcctgcc gccccgacgt tggctgcgag ccctgggcct tcacccgaac ttggggggtg 2941 gggtggggaa aaggaagaaa cgcgggcgta ttggccccaa tggggtctcg gtggggtatc 3001 gacagagtgc cagccctggg accgaacccc gcgtttatga acaaacgacc caacaccgtg 3061 cgttttattc tgtcttttta ttgccgtcat agcgcgggtt ccttccggta ttgtctcctt 3121 ccgtgtttca gttagcctcc ccctagggtg ggcgaagaac tccagcatga gatccccgcg 3181 ctggaggatc atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa 3241 ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc 3301 gaaccccaga gtcccgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc 3361 gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc 3421 tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcgatccgc cacacccagc 3481 cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag 3541 gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgctcgcctt gagcctggcg 3601 aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga 3661 ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg 3721 caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc 3781 tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc 3841 cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg 3901 gccagccacg atagccgcgc tgcctcgtct tgcagttcat tcagggcacc ggacaggtcg 3961 gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag 4021 cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga 4081 gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga 4141 tcgatctttg caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc 4201 agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg 4261 agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat 4321 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga 4381 ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg 4441 ggagcctggg gactttccac accctaactg acacacattc cacagctggt tctttccgcc 4501 tcaggactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 4561 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 4621 cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt 4681 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc 4741 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct 4801 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat 4861 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc 4921 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc 4981 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg 5041 atttaacaaa aatttaacgc gaatttt //