LOCUS       V041016                10912 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V041016
VERSION     V041016
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..5568
                     /label="EIF4G3(NM_001391904)"
                     /note="EIF4G3(NM_001391904)"
                     /gene="EIF4G3"
     CDS             5575..5664
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     misc_feature    5688..6276
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    6342..6390
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6592..7020
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        7034..7363
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             7430..8224
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    8400..8521
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(8570..8586)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8594..8610
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8618..8648)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8663..8684
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8972..9560)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9731..10591)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10592..10696)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgaa ttcacaacct
      721 caaacccgtt ctccgttttt ccagaggcct caaatacagc ctcctagagc taccatcccg
      781 aacagcagtc cttccattcg tcctggtgca cagacaccca ctgcagtgta ccaggctaat
      841 cagcacatca tgatggttaa ccatctgccc atgccgtacc cagtgcccca ggggcctcag
      901 tactgtatac cacagtaccg tcatagtggc cctccttatg ttgggccccc ccaacaatat
      961 ccagttcaac caccggggcc aggtcctttt tatcctggac caggacctgg ggacttcccc
     1021 aatgcttatg gaacgccttt ttacccaagt cagccggtgt atcagtcagc acctatcata
     1081 gtgcctacgc agcaacagcc gcctccagcc aagagagaga aaaaaactat aagaattcgg
     1141 gatccaaacc agggaggtaa agacataaca gaggagatta tgtctggagg tggcagcaga
     1201 aatcctactc cacccatagg aagacccacg tccacaccta ctcctcctca gctgcccagc
     1261 caggtccccg agcacagccc tgtggtttat gggactgtgg agagcgctca tcttgctgcc
     1321 agcacccctg tcactgcagc tagcgaccag aagcaagagg agaagccaaa accagatcca
     1381 gtgttaaagt ctccttcccc agtccttagg ctagtcctca gtggagagaa gaaagaacaa
     1441 gaaggccaga catctgaaac tactgcaata gtatccatag cagagcttcc tctgcctcca
     1501 tcacctacca ctgtttcttc tgttgctcga agtacaattg cagcccccac ctcttctgct
     1561 cttagtagcc aaccaatatt caccactgct atagatgaca gatgtgaact ctcatcccca
     1621 agagaagaca caattcctat acccagcctc acatcttgca cagaaacatc agacccttta
     1681 ccaacaaatg aaaatgatga tgatatatgc aagaaaccct gtagtgtagc acctaatgat
     1741 attccactgg tttctagtac taacctaatt aatgaaataa atggagttag cgaaaaatta
     1801 tcagccacgg agagcattgt ggaaatagta aaacaggaag tattgccatt gactcttgaa
     1861 ttggagattc tcgaaaatcc cccagaagaa atgaaactgg agtgtatccc agctcccatc
     1921 accccttcca cagttccttc ctttcctcca actcctccaa ctcctccagc ttctcctcct
     1981 cacactccag tcattgttcc tgctgctgcc actactgtta gttctccgag tgctgccatc
     2041 acagtccaga gagtcctaga ggaggacgag agcataagaa cttgccttag tgaagatgca
     2101 aaagagattc agaacaaaat agaggtagaa gcagatgggc aaacagaaga gattttggat
     2161 tctcaaaact taaattcaag aaggagccct gtcccagctc aaatagctat aactgtacca
     2221 aagacatgga agaaaccaaa agatcggacc cgaaccactg aagagatgtt agaggcagaa
     2281 ttggagctta aagctgaaga ggagctttcc attgacaaag tacttgaatc tgaacaagat
     2341 aaaatgagcc aggggtttca tcctgaaaga gacccctctg acctaaaaaa agtgaaagct
     2401 gtggaagaaa atggagaaga agctgagcca gtacgtaatg gtgctgagag tgtttctgag
     2461 ggtgaaggaa tagatgctaa ttcaggctcc acagatagtt ctggtgatgg ggttacattt
     2521 ccatttaaac cagaatcctg gaagcctact gatactgaag gtaagaagca gtatgacagg
     2581 gagtttctgc tggacttcca gttcatgcct gcctgtatac aaaaaccaga gggcctgcct
     2641 cctatcagtg atgtggttct tgacaagatc aaccaaccca aattgccaat gcgaactctg
     2701 gatcctcgaa ttttgcctcg aggaccagac tttacaccag cctttgctga ttttggaagg
     2761 cagacacctg gtggaagagg cgtacctatt tgcaaagtgc agagcaggca tggattgcca
     2821 attctggaac agagcaaagc cccaacttgc cctccactgg tgatgtcaca cccacccatg
     2881 aagagcctgc ctctagggtt gttgaatgtt gggtcacgaa gatctcaacc tggccaaaga
     2941 agagaaccca gaaagatcat cacagtttct gtaaaagaag atgtacacct gaaaaaggca
     3001 gaaaatgcct ggaagccaag ccaaaaacga gacagccaag ccgatgatcc cgaaaacatt
     3061 aaaacccagg agctttttag aaaagttcga agtatcttaa ataaattgac accacagatg
     3121 ttcaatcaac tgatgaagca agtgtcagga cttactgttg acacagagga gcggctgaaa
     3181 ggagttattg acctggtctt tgagaaggct attgatgaac ccagtttctc tgtggcttac
     3241 gcaaacatgt gtcgatgtct agtaacgctg aaagtaccca tggcagacaa gcctggtaac
     3301 acagtgaatt tccggaagct gctactgaac cgttgccaga aggagtttga aaaagataaa
     3361 gcagatgatg atgtctttga gaagaagcag aaagaacttg aggctgccag tgctccagag
     3421 gagaggacaa ggcttcatga tgaactggaa gaagccaagg acaaagcccg gcggagatcc
     3481 attggcaaca tcaagtttat tggagaactc tttaaactca aaatgctgac tgaagccatc
     3541 atgcatgact gtgtggtgaa gctgctaaag aaccatgatg aagaatccct ggagtgcctg
     3601 tgtcgcctgc tcaccaccat tggcaaagac ttggactttg aaaaagcaaa gccacgtatg
     3661 gaccagtact ttaatcagat ggagaaaatt gtgaaagaaa gaaaaacctc atctaggatt
     3721 cggttcatgc ttcaagatgt tatagaccta aggctgtgca attgggtatc tcgaagagca
     3781 gatcaagggc ctaaaactat cgaacagatt cacaaagagg ctaaaataga agaacaagaa
     3841 gagcaaagga aggtccagca actcatgacc aaagagaaga gaagaccagg tgtccagaga
     3901 gtggacgaag gtgggtggaa cactgtacaa ggggccaaga acagtcgggt actggacccc
     3961 tcaaaattcc taaaaatcac taagcctaca attgatgaaa aaattcagct ggtacctaaa
     4021 gcacagctag gcagctgggg aaaaggcagc agtggtggag caaaggcaag tgagactgat
     4081 gccttacggt caagtgcttc cagtttaaac agattctctg ccctgcaacc tccagcaccc
     4141 tcagggtcca cgccatccac gcctgtagag tttgattccc gaaggacctt aactagtcgt
     4201 ggaagtatgg gcagggagaa gaatgacaag ccccttccat ctgcaacagc tcggccaaat
     4261 actttcatga ggggtggcag cagtaaagac ctgctagaca atcagtctca agaagagcag
     4321 cggagagaga tgctggagac cgtgaagcag ctcacaggag gtgtggatgt ggagaggaac
     4381 agcactgagg ctgagcgaaa taaaacaagg gagtcagcaa aaccagaaat ttcagcaatg
     4441 tcagctcatg acaaggctgc attatcagaa gaggaactgg agaggaagtc gaaatctatc
     4501 attgatgaat ttctacacat taatgatttt aaggaagcca tgcagtgtgt ggaagagctg
     4561 aatgcccagg gcctactaca tgtttttgtg agagtgggag tggagtccac cctggaaagg
     4621 agccagatca ccagggatca catgggccaa ttactctatc agctggtaca gtcagaaaaa
     4681 ctcagcaaac aggacttttt caaaggtttt tcagaaactt tggaattggc agatgacatg
     4741 gccattgata ttccccatat ttggttgtac cttgctgaac tggtgacccc catgttaaaa
     4801 gaaggtggaa tctccatgag agaacttacc atagaattta gcaaaccttt acttcctgtt
     4861 ggaagagctg gggtcttgct atctgaaata ttgcacctac tatgcaaaca aatgagccat
     4921 aagaaagtgg gagccttatg gagggaggct gacctcagct ggaaggactt tttaccagaa
     4981 ggagaagatg tacataattt tcttttggag cagaagttgg acttcataga gtctgacagt
     5041 ccctgttcct ctgaagcact ttcaaagaaa gaactgtctg ccgaagagct gtataagcga
     5101 ctcgagaaac tcattattga ggacaaagcg aatgatgaac agatctttga ctgggtagag
     5161 gctaatctag acgaaatcca gatgagttca cctacattcc ttagagcttt aatgactgct
     5221 gtttgtaaag cagctattat agccgactct tctaccttca gagtggacac tgctgttatc
     5281 aagcagagag tgccgatctt actcaagtac ctagactcag atacagagaa ggaactgcaa
     5341 gcactttatg cactacaagc atcgatagta aaacttgatc aacctgccaa tttgctgcgg
     5401 atgttttttg attgtctata tgacgaggag gtgatctccg aggatgcctt ctacaaatgg
     5461 gagagcagca aggaccctgc agagcagaat gggaagggcg tggctctgaa atctgtcacg
     5521 gcattcttca cgtggctgcg ggaagcagaa gaggagtctg aggataacct cgaggaacaa
     5581 aaactaatat cagaggaaga tttggaacag aaactaatca gtgaagaaga tttagagcaa
     5641 aagttaattt ccgaggagga cttatagtga gggcccgata tctcgacaat caacctctgg
     5701 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     5761 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     5821 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     5881 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     5941 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     6001 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     6061 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
     6121 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     6181 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     6241 agacgagtcg gatctccctt tgggccgcct ccccgcctgg aaacggggga ggctaactga
     6301 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa
     6361 taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc
     6421 actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt
     6481 ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg
     6541 caggccctgc catagcagat ctgcgcagct ggggctctag ggggtatccc cacgcgccct
     6601 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     6661 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     6721 gctttccccg tcaagctcta aatcggggca tccctttagg gttccgattt agtgctttac
     6781 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     6841 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     6901 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     6961 tggggatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     7021 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     7081 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     7141 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     7201 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     7261 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     7321 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     7381 tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     7441 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     7501 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     7561 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
     7621 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     7681 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     7741 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     7801 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     7861 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     7921 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
     7981 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     8041 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     8101 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     8161 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     8221 ctgagcggga ctctggggtt cgcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     8281 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     8341 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     8401 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     8461 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     8521 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     8581 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     8641 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     8701 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     8761 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     8821 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     8881 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     8941 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     9001 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     9061 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     9121 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta
     9181 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     9241 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     9301 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     9361 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     9421 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     9481 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     9541 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     9601 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     9661 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     9721 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     9781 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     9841 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     9901 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     9961 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
    10021 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
    10081 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
    10141 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
    10201 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
    10261 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
    10321 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
    10381 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    10441 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    10501 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    10561 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    10621 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    10681 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
    10741 atctcccgat cccctatggt cgactctcag tacaatctgc tctgatgccg catagttaag
    10801 ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta
    10861 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gg
//
