LOCUS V035318 7027 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V035318 VERSION V035318 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 706..1683 /label="ELAVL1(NM_001419)" /note="ELAVL1(NM_001419)" /gene="ELAVL1" CDS 1690..1779 /codon_start=1 /product="3 tandem Myc epitope tags" /transl_table=1 /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL" /label="3xMyc" misc_feature 1803..2391 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 2457..2505 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 2707..3135 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3149..3478 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3545..4339 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4515..4636 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4685..4701) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4709..4725 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4733..4763) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4778..4799 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5087..5675) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5846..6706) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6707..6811) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg 661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgtc taatggttat 721 gaagaccaca tggccgaaga ctgcaggggt gacatcggga gaacgaattt gatcgtcaac 781 tacctccctc agaacatgac ccaggatgag ttacgaagcc tgttcagcag cattggtgaa 841 gttgaatctg caaaacttat tcgggataaa gtagcaggac acagcttggg ctatggcttt 901 gtgaactacg tgaccgcgaa ggatgcagag agagcgatca acacgctgaa cggcttgagg 961 ctccagtcaa aaaccattaa ggtgtcgtat gctcgcccga gctcagaggt gatcaaagac 1021 gccaacttgt acatcagcgg gctcccgcgg accatgaccc agaaggacgt agaagacatg 1081 ttctctcggt ttgggcggat catcaactcg cgggtcctcg tggatcagac tacaggtttg 1141 tccagagggg ttgcgtttat ccggtttgac aaacggtcgg aggcagaaga ggcaattacc 1201 agtttcaatg gtcataaacc cccaggttcc tctgagccca tcacagtgaa gtttgcagcc 1261 aaccccaacc agaacaaaaa cgtggcactc ctctcgcagc tgtaccactc gccagcgcga 1321 cggttcggag gccccgttca ccaccaggcg cagagattca ggttctcccc catgggcgtc 1381 gatcacatga gcgggctctc tggcgtcaac gtgccaggaa acgcctcctc cggctggtgc 1441 attttcatct acaacctggg gcaggatgcc gacgagggga tcctctggca gatgtttggg 1501 ccgtttggtg ccgtcaccaa tgtgaaagtg atccgcgact tcaacaccaa caagtgcaaa 1561 gggtttggct ttgtgaccat gacaaactat gaagaagccg cgatggccat agccagcctg 1621 aacggctacc gcctggggga caaaatctta caggtttcct tcaaaaccaa caagtcccac 1681 aaactcgagg aacaaaaact aatatcagag gaagatttgg aacagaaact aatcagtgaa 1741 gaagatttag agcaaaagtt aatttccgag gaggacttat agtgagggcc cgatatctcg 1801 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg 1861 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc 1921 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt 1981 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca 2041 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc 2101 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc 2161 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc 2221 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc 2281 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc 2341 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaaacg 2401 ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc 2461 aataaaaaga cagaataaaa cgcacgggtg ttgggtcgtt tgttcataaa cgcggggttc 2521 ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc 2581 gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag 2641 ccaacgtcgg ggcggcaggc cctgccatag cagatctgcg cagctggggc tctagggggt 2701 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg 2761 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc 2821 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg gggcatccct ttagggttcc 2881 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta 2941 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta 3001 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg 3061 atttataagg gattttgggg atttcggcct attggttaaa aaatgagctg atttaacaaa 3121 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg 3181 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg 3241 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc 3301 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca 3361 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc 3421 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa 3481 gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt 3541 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct 3601 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct 3661 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga 3721 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc 3781 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg 3841 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc 3901 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca 3961 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga 4021 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc 4081 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga 4141 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca 4201 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg 4261 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct 4321 tcttgacgag ttcttctgag cgggactctg gggttcgcga aatgaccgac caagcgacgc 4381 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg 4441 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt 4501 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca 4561 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac 4621 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat 4681 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 4741 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa 4801 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat 4861 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 4921 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 4981 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 5041 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 5101 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 5161 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 5221 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 5281 aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 5341 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 5401 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 5461 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 5521 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 5581 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 5641 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 5701 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 5761 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 5821 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 5881 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 5941 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 6001 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 6061 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 6121 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 6181 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 6241 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 6301 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 6361 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 6421 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 6481 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 6541 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 6601 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 6661 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 6721 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 6781 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 6841 tcgacggatc gggagatctc ccgatcccct atggtcgact ctcagtacaa tctgctctga 6901 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg 6961 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct 7021 gcttagg //