LOCUS       V020041                 9305 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V020041
VERSION     V020041
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             683..3964
                     /label="FBH1(NM_032807)"
                     /note="FBH1(NM_032807)"
                     /gene="FBH1"
     CDS             3971..3997
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4001..4027
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4031..4057
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     misc_feature    4081..4669
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    4735..4783
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      4985..5413
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5427..5756
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5823..6617
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6793..6914
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6963..6979)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6987..7003
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7011..7041)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7056..7077
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7365..7953)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8124..8984)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8985..9089)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccgcca ccatgagcta cgaggtgact tcaggctgcc attggacctg
      721 tcaagtgcct gagtcatgtg ataatgggct acattgcgca gggcccctgg gccatctcca
      781 caggagatgc cagaggacga gtgcccactt gctggtcttc acagagcacg ctgaaatgag
      841 acggtttaag cggaagcatc ttactgccat tgactgccag catttggctc ggagtcactt
      901 ggctgtgacc cagcccttcg gtcaaagatg gacaaacaga gatccgaacc atggtctcta
      961 tcctaaaccg agaacaaaaa gagggagtag gggtcaggga agtcaaagat gcatccctga
     1021 gttcttccta gcaggcaagc agccgtgcac caatgacatg gccaaaagca attctgttgg
     1081 ccaggacagc tgtcaggact ctgagggtga catgatcttt cctgcagaga gcagctgtgc
     1141 actgcctcag gaaggcagtg cagggccggg ctcaccaggg tctgccccgc cctccaggaa
     1201 gcggtcttgg tcctctgagg aagagagtaa ccaggctacc gggaccagcc ggtgggatgg
     1261 agtttctaag aaagctccac ggcaccattt gtctgtgcca tgcacaaggc ctagggaggc
     1321 caggcaagaa gcagaggaca gtacgtctcg gctctctgcg gagtctggtg aaaccgacca
     1381 agatgctggg gacgtgggtc ctgatcccat tcctgactca tactatgggc ttcttgggac
     1441 cttgccctgc caggaagcac tgagccacat ttgcagcctg cctagtgagg tcctgaggca
     1501 cgtgtttgcc ttcctcccgg tggaagacct ctattggaac ctgagcttgg tgtgccactt
     1561 gtggagggag atcatcagtg acccgctgtt cattccttgg aagaagctgt accatcgata
     1621 cctgatgaat gaagagcaag ctgtcagcaa agtggacggc atcctgtcta actgtggcat
     1681 agaaaaggag tcagacctgt gtgtgctgaa cctcatacga tacacagcca ccactaagtg
     1741 ctctccgagt gtggatcccg agagggtgct gtggagtctg agggaccacc ccctcctccc
     1801 cgaggctgag gcgtgtgtgc ggcaacacct ccccgacctc tacgctgctg ccgggggtgt
     1861 caacatctgg gccctggtgg cggctgtggt gctcctctcc agcagtgtga atgacatcca
     1921 gcgactgctc ttctgcctcc ggagacccag ctccacggtg accatgccag atgtcaccga
     1981 gaccctgtac tgcatagccg tgcttctcta cgccatgagg gagaagggga ttaacatcag
     2041 caataggatt cactacaaca ttttctattg cctatatctt caggagaatt cctgcactca
     2101 ggccacaaaa gttaaagagg agccatctgt ctggccaggc aagaaaacca tccaacttac
     2161 acatgaacaa cagctgattc tgaatcacaa gatggaacct ctccaggtgg tgaaaattat
     2221 ggcctttgcc ggcactggga agacctcaac gctggtcaag tatgcagaga agtggtctca
     2281 gagcaggttt ctgtatgtga cattcaacaa gagcatcgca aagcaggccg aacgcgtctt
     2341 ccccagcaac gtcatctgca aaaccttcca ctccatggcc tacgggcaca tagggcggaa
     2401 gtaccagtca aagaagaagt tgaatctctt caagttaaca cccttcatgg tcaactccgt
     2461 ccttgctgaa gggaagggtg gattcataag agccaagctt gtgtgtaaga ctctagaaaa
     2521 cttctttgcc tcggctgacg aagagctgac cattgatcac gtgcctattt ggtgtaagaa
     2581 cagccaagga cagagagtca tggttgagca gagtgaaaaa ctgaatggtg tccttgaagc
     2641 gagccgcctc tgggataaca tgcggaagct gggggagtgc acagaagagg cgcaccagat
     2701 gactcatgac ggctacttga aactctggca gctgagcaag ccttcgctgg cctcttttga
     2761 cgccatcttt gtggatgagg cccaggactg cacaccagct atcatgaaca tagttctgtc
     2821 tcagccatgt gggaaaatct ttgtagggga cccgcaccag cagatctata ccttccgggg
     2881 tgcggtcaac gccctgttca cagtgcccca cacccacgtc ttctatctca cgcagagttt
     2941 tcggtttggt gtggaaatag cttatgtggg agctactatc ttggatgttt gcaagagagt
     3001 caggaaaaag actttggttg gaggaaacca tcagagtggc attagaggtg acgcaaaggg
     3061 gcaagtggcc ttgttgtccc ggaccaacgc caacgtgttt gatgaggccg tacgggtgac
     3121 ggaaggggaa ttcccttcaa ggatacattt gattgggggg attaaatcat ttggattgga
     3181 cagaatcatt gatatttgga tccttcttca gccagaggaa gaacggagga aacaaaacct
     3241 cgtcattaaa gacaaattta tcagaagatg ggtgcacaaa gaaggcttta gtggcttcaa
     3301 gaggtatgtg accgctgccg aggacaagga gcttgaagcc aagatcgcag ttgttgaaaa
     3361 gtataacatc aggattccag agctggtgca aaggatagaa aaatgccata tagaagattt
     3421 ggactttgca gagtacattc tgggcactgt gcacaaagcc aaaggcctgg agtttgacac
     3481 tgtgcatgtt ttggatgatt ttgtgaaagt gccttgtgcc cggcataacc tgccccagct
     3541 tccgcacttc agagttgagt cattttctga ggatgaatgg aatttactgt atgttgcagt
     3601 aactcgagcc aagaagcgtc tcatcatgac caaatcattg gaaaacattt tgactttggc
     3661 tggggagtac ttcttgcaag cagagctgac aagcaacgtc ttaaaaacag gcgtggtgcg
     3721 ctgctgcgtg ggacagtgca acaatgccat ccctgttgac accgtcctta ccatgaagaa
     3781 gctgcccatc acctatagca acaggaagga aaacaagggg ggctacctct gccactcctg
     3841 tgcggagcag cgcatcgggc ccctggcgtt cctgacagcc tccccggagc aggtgcgcgc
     3901 catggagcgc actgtggaga acatcgtact gccccggcat gaggccctgc tcttcctcgt
     3961 cttcctcgag tacccatacg acgtacctga ttatgcaact tatccttatg atgtacctga
     4021 ttatgctagc tatccttatg atgtcccaga ctacgcatag tgagggcccg atatctcgac
     4081 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     4141 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     4201 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     4261 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     4321 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     4381 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     4441 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     4501 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     4561 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     4621 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaaacggg
     4681 ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa
     4741 taaaaagaca gaataaaacg cacgggtgtt gggtcgtttg ttcataaacg cggggttcgg
     4801 tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc
     4861 gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc
     4921 aacgtcgggg cggcaggccc tgccatagca gatctgcgca gctggggctc tagggggtat
     4981 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     5041 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     5101 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg gcatcccttt agggttccga
     5161 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     5221 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     5281 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     5341 ttataaggga ttttggggat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     5401 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     5461 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     5521 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     5581 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     5641 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     5701 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     5761 tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc
     5821 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     5881 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     5941 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     6001 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     6061 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     6121 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     6181 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     6241 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     6301 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
     6361 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     6421 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     6481 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     6541 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     6601 ttgacgagtt cttctgagcg ggactctggg gttcgcgaaa tgaccgacca agcgacgccc
     6661 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     6721 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     6781 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     6841 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     6901 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     6961 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     7021 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     7081 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     7141 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     7201 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     7261 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     7321 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     7381 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     7441 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     7501 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa
     7561 tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     7621 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     7681 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     7741 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     7801 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     7861 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     7921 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     7981 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     8041 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     8101 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     8161 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     8221 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     8281 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     8341 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     8401 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     8461 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     8521 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     8581 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     8641 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     8701 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     8761 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     8821 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     8881 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     8941 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     9001 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     9061 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     9121 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
     9181 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
     9241 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
     9301 ttagg
//
