LOCUS V020041 9305 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V020041 VERSION V020041 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 683..3964 /label="FBH1(NM_032807)" /note="FBH1(NM_032807)" /gene="FBH1" CDS 3971..3997 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 4001..4027 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 4031..4057 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" misc_feature 4081..4669 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 4735..4783 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 4985..5413 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 5427..5756 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 5823..6617 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 6793..6914 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(6963..6979) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6987..7003 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7011..7041) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7056..7077 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7365..7953) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8124..8984) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8985..9089) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg 661 taccgagctc ggatccgcca ccatgagcta cgaggtgact tcaggctgcc attggacctg 721 tcaagtgcct gagtcatgtg ataatgggct acattgcgca gggcccctgg gccatctcca 781 caggagatgc cagaggacga gtgcccactt gctggtcttc acagagcacg ctgaaatgag 841 acggtttaag cggaagcatc ttactgccat tgactgccag catttggctc ggagtcactt 901 ggctgtgacc cagcccttcg gtcaaagatg gacaaacaga gatccgaacc atggtctcta 961 tcctaaaccg agaacaaaaa gagggagtag gggtcaggga agtcaaagat gcatccctga 1021 gttcttccta gcaggcaagc agccgtgcac caatgacatg gccaaaagca attctgttgg 1081 ccaggacagc tgtcaggact ctgagggtga catgatcttt cctgcagaga gcagctgtgc 1141 actgcctcag gaaggcagtg cagggccggg ctcaccaggg tctgccccgc cctccaggaa 1201 gcggtcttgg tcctctgagg aagagagtaa ccaggctacc gggaccagcc ggtgggatgg 1261 agtttctaag aaagctccac ggcaccattt gtctgtgcca tgcacaaggc ctagggaggc 1321 caggcaagaa gcagaggaca gtacgtctcg gctctctgcg gagtctggtg aaaccgacca 1381 agatgctggg gacgtgggtc ctgatcccat tcctgactca tactatgggc ttcttgggac 1441 cttgccctgc caggaagcac tgagccacat ttgcagcctg cctagtgagg tcctgaggca 1501 cgtgtttgcc ttcctcccgg tggaagacct ctattggaac ctgagcttgg tgtgccactt 1561 gtggagggag atcatcagtg acccgctgtt cattccttgg aagaagctgt accatcgata 1621 cctgatgaat gaagagcaag ctgtcagcaa agtggacggc atcctgtcta actgtggcat 1681 agaaaaggag tcagacctgt gtgtgctgaa cctcatacga tacacagcca ccactaagtg 1741 ctctccgagt gtggatcccg agagggtgct gtggagtctg agggaccacc ccctcctccc 1801 cgaggctgag gcgtgtgtgc ggcaacacct ccccgacctc tacgctgctg ccgggggtgt 1861 caacatctgg gccctggtgg cggctgtggt gctcctctcc agcagtgtga atgacatcca 1921 gcgactgctc ttctgcctcc ggagacccag ctccacggtg accatgccag atgtcaccga 1981 gaccctgtac tgcatagccg tgcttctcta cgccatgagg gagaagggga ttaacatcag 2041 caataggatt cactacaaca ttttctattg cctatatctt caggagaatt cctgcactca 2101 ggccacaaaa gttaaagagg agccatctgt ctggccaggc aagaaaacca tccaacttac 2161 acatgaacaa cagctgattc tgaatcacaa gatggaacct ctccaggtgg tgaaaattat 2221 ggcctttgcc ggcactggga agacctcaac gctggtcaag tatgcagaga agtggtctca 2281 gagcaggttt ctgtatgtga cattcaacaa gagcatcgca aagcaggccg aacgcgtctt 2341 ccccagcaac gtcatctgca aaaccttcca ctccatggcc tacgggcaca tagggcggaa 2401 gtaccagtca aagaagaagt tgaatctctt caagttaaca cccttcatgg tcaactccgt 2461 ccttgctgaa gggaagggtg gattcataag agccaagctt gtgtgtaaga ctctagaaaa 2521 cttctttgcc tcggctgacg aagagctgac cattgatcac gtgcctattt ggtgtaagaa 2581 cagccaagga cagagagtca tggttgagca gagtgaaaaa ctgaatggtg tccttgaagc 2641 gagccgcctc tgggataaca tgcggaagct gggggagtgc acagaagagg cgcaccagat 2701 gactcatgac ggctacttga aactctggca gctgagcaag ccttcgctgg cctcttttga 2761 cgccatcttt gtggatgagg cccaggactg cacaccagct atcatgaaca tagttctgtc 2821 tcagccatgt gggaaaatct ttgtagggga cccgcaccag cagatctata ccttccgggg 2881 tgcggtcaac gccctgttca cagtgcccca cacccacgtc ttctatctca cgcagagttt 2941 tcggtttggt gtggaaatag cttatgtggg agctactatc ttggatgttt gcaagagagt 3001 caggaaaaag actttggttg gaggaaacca tcagagtggc attagaggtg acgcaaaggg 3061 gcaagtggcc ttgttgtccc ggaccaacgc caacgtgttt gatgaggccg tacgggtgac 3121 ggaaggggaa ttcccttcaa ggatacattt gattgggggg attaaatcat ttggattgga 3181 cagaatcatt gatatttgga tccttcttca gccagaggaa gaacggagga aacaaaacct 3241 cgtcattaaa gacaaattta tcagaagatg ggtgcacaaa gaaggcttta gtggcttcaa 3301 gaggtatgtg accgctgccg aggacaagga gcttgaagcc aagatcgcag ttgttgaaaa 3361 gtataacatc aggattccag agctggtgca aaggatagaa aaatgccata tagaagattt 3421 ggactttgca gagtacattc tgggcactgt gcacaaagcc aaaggcctgg agtttgacac 3481 tgtgcatgtt ttggatgatt ttgtgaaagt gccttgtgcc cggcataacc tgccccagct 3541 tccgcacttc agagttgagt cattttctga ggatgaatgg aatttactgt atgttgcagt 3601 aactcgagcc aagaagcgtc tcatcatgac caaatcattg gaaaacattt tgactttggc 3661 tggggagtac ttcttgcaag cagagctgac aagcaacgtc ttaaaaacag gcgtggtgcg 3721 ctgctgcgtg ggacagtgca acaatgccat ccctgttgac accgtcctta ccatgaagaa 3781 gctgcccatc acctatagca acaggaagga aaacaagggg ggctacctct gccactcctg 3841 tgcggagcag cgcatcgggc ccctggcgtt cctgacagcc tccccggagc aggtgcgcgc 3901 catggagcgc actgtggaga acatcgtact gccccggcat gaggccctgc tcttcctcgt 3961 cttcctcgag tacccatacg acgtacctga ttatgcaact tatccttatg atgtacctga 4021 ttatgctagc tatccttatg atgtcccaga ctacgcatag tgagggcccg atatctcgac 4081 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct 4141 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt 4201 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg 4261 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact 4321 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct 4381 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg 4441 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc 4501 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc 4561 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt 4621 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaaacggg 4681 ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa 4741 taaaaagaca gaataaaacg cacgggtgtt gggtcgtttg ttcataaacg cggggttcgg 4801 tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc 4861 gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc 4921 aacgtcgggg cggcaggccc tgccatagca gatctgcgca gctggggctc tagggggtat 4981 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg 5041 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc 5101 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg gcatcccttt agggttccga 5161 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt 5221 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat 5281 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat 5341 ttataaggga ttttggggat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa 5401 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct 5461 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa 5521 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa 5581 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt 5641 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct 5701 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc 5761 tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc 5821 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat 5881 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt 5941 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac 6001 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg 6061 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc 6121 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa 6181 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc 6241 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg 6301 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg 6361 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa 6421 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg 6481 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct 6541 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc 6601 ttgacgagtt cttctgagcg ggactctggg gttcgcgaaa tgaccgacca agcgacgccc 6661 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga 6721 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc 6781 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 6841 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 6901 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca 6961 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga 7021 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt 7081 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga 7141 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc 7201 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 7261 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 7321 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 7381 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 7441 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 7501 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa 7561 tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 7621 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 7681 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 7741 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 7801 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 7861 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 7921 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 7981 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 8041 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 8101 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 8161 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 8221 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 8281 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 8341 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 8401 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc 8461 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 8521 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 8581 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 8641 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 8701 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca 8761 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 8821 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 8881 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 8941 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 9001 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 9061 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc 9121 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 9181 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 9241 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 9301 ttagg //