LOCUS       62056_9270        6439 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6439)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6439
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..330
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        331..534
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          672..768
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             832..855
                     /codon_start=1
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /translation="DYKDDDDK"
     polyA_signal    complement(3578..3712)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3823..3839)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3847..3863)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3871..3901)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3916..3937)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4225..4813)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4987..5844)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5845..5949)
                     /label=AmpR promoter
     primer_bind     6423..6439
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
       61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag
      661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
      721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
      781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaccat ggactacaag
      841 gacgacgatg acaagcttat ggcggcggcg ggggtcgccg aggggcggcg cgcggccctg
      901 gaggcggctg cggcggtggc tcctgagcgg ggcggcggga gctgcgtgct atgctgcgga
      961 gacctggagg ccacggcgct gggccgctgc gaccacccgg tgtgctaccg ctgctccacc
     1021 aagatgcggg tgctgtgcga gcagcgctac tgcgccgtgt gccgcgagga gctgcgccag
     1081 gtggtgtttg ggaagaagct tcctgccttt gccagcatcc ccatccacca gctgcagcat
     1141 gagaagaaat acgatatcta ctttgcagat ggaaaggtgt atgcattgta caggcagctg
     1201 ctgcagcacg agtgcccgcg gtgccccgag ctgccgcctt tcagcctctt cggggacctg
     1261 gagcagcaca tgcggaggca gcacgagctc ttctgctgcc ggctgtgcct ccagcacctc
     1321 cagatcttca catacgaacg caagtggtac tcgcgcaagg acctggcccg gcatcgcatg
     1381 cagggggacc ccgatgacac gtcgcaccgc gggcacccgc tctgcaagtt ctgtgatgag
     1441 cgctacctgg acaatgatga gctgcttaag cacctgcgcc gcgaccacta cttctgccac
     1501 ttctgcgact cggacggggc ccaggactac tacagcgact atgcctacct gcgcgagcac
     1561 ttccgggaga agcacttcct gtgtgaggag ggccgctgca gcacagagca gttcacccac
     1621 gccttccgca ccgagatcga cctcaaggcc cacaggacgg cctgccacag ccgcagccgt
     1681 gccgaggcgc gccagaaccg ccagatcgac ctacagttca gctacgcgcc acggcactcg
     1741 cgccggaacg agggggtcgt cggtggcgaa gactacgagg aggtggacag gtacagccgc
     1801 cagggccgag tggcccgggc cggcactcgc ggagcccagc agagccgccg aggaagctgg
     1861 aggtacaaaa gggaggaaga ggaccgagaa gtagcagctg ctgtccgggc ttccgtggct
     1921 gcacagcagc aggaggaggc acgcaggagt gaggaccggg aggaaggcgg ccggcccaag
     1981 aaggaggagg tagcagtgcg gggtcctgag gagccccgtg gcccccggcg cccaccccgg
     2041 actcagggtg aaggcccagg ccccaaggaa acctcgacaa atggtcctgt aagccaagag
     2101 gccttcccag tgacgggcgc agccacccca tgcaccctcc caccacccag ccctaagctc
     2161 aaggacgaag acttccccag cctctctgcc tccacttcct cctgctgctc cactgcagca
     2221 accccgggcc ctgtggggtt ggcactgcca tacgccatcc ctgccagggg caggagtgcc
     2281 ttccaggagg aggatttccc tgccctggtg tcctcggtgc ccaagcctgg caccaccccc
     2341 accagcctcg tctctgcctg gaacagcagc agcagcagca agaaggtagc acagccccca
     2401 ccctcagcac aggctaccgg aagcggccag cccaccagaa aggctgggaa ggggagcagg
     2461 ggcggcagga agggcggccc gcccgtcaca caggaggagg agggtggcag tccggccgct
     2521 caggagcttc taagcacacg ccccacgggc tccatctcct ccccactggg tccggtctcc
     2581 atccagccct ctaaagttgg caagaagaag aaagtgggct cagagaagcc tggcaccaca
     2641 ctgccacagc ccctgcccgc tagctgtccc cctgggccta tgcaggcccc agaagctcct
     2701 gccagcagag ccgaggggcc agttgctgtt gtcgttaatg gacacgcaga gggtccagcc
     2761 ccagctcgga gtgcccccaa ggaaccccca gggctcccaa ggcccctggg gtccttcccc
     2821 tgctccacgc cacaggagga cttcccagcg cttggtggcc cctgtccgcc ccggatgcca
     2881 ccacccccag gcttcagcgc tgtggtgctc ctgaagggca cacctccccc acccccacca
     2941 ggcctggtgc cccccgtcag caagccgccc cccggcttct ctggccttct gcctagcccc
     3001 cacccggcct gcgttcccag ccccaccacc accgccacta ccacaaaacc acccaggccg
     3061 ccgcctgccc tgcaggccta cctagtcccc gagaacttcc gggagaggaa cctgcagctc
     3121 atccagtcca tcagggactt cctgcagagc gacgaggccc gcttcagcga gttcaagagc
     3181 cactcagggg agttcagaca gggcctgatc tccgcagccc agtattacaa gagttgccgg
     3241 gacctgctgg gggagaattt ccagaaggtc tttaacgagc tgctggtcct actgcccgac
     3301 acagccaagc agcaggagct cctgtctgca cacacggact tctgcaaccg tgagaagcct
     3361 ctcggcacca agtccaagaa aaataagaag agcgcgtggc aggccaccac ccagcaggcg
     3421 ggcctggact gccgtgtgtg ccccacctgc cagcaggtgc tcgcacacgg cgatgccagc
     3481 agccaccagg cgctgcatgc tgcccgggac gacgacttcc cctccctgca agccatcgcc
     3541 aggatcatca cgtagctcga ggtaccgcgg ccgcggggat ccagacatga taagatacat
     3601 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     3661 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     3721 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttcggatcc
     3781 tctagagtcg atctgcaggc atgctagctt ggcgtaatca tggtcatagc tgtttcctgt
     3841 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     3901 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     3961 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     4021 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     4081 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     4141 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     4201 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     4261 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     4321 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     4381 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     4441 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     4501 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     4561 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     4621 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     4681 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     4741 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     4801 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     4861 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     4921 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     4981 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     5041 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     5101 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     5161 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     5221 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     5281 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     5341 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     5401 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     5461 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     5521 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     5581 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     5641 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     5701 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     5761 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     5821 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     5881 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     5941 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     6001 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
     6061 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     6121 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     6181 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
     6241 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct
     6301 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     6361 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     6421 ttgtaaaacg acggccagt
//
