LOCUS       V025445                11974 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V025445
VERSION     V025445
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..6633
                     /label="FRAS1(NM_001166133)"
                     /note="FRAS1(NM_001166133)"
                     /gene="FRAS1"
     CDS             6640..6666
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             6670..6696
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             6700..6726
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     misc_feature    6750..7338
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    7404..7452
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      7654..8082
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        8096..8425
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             8492..9286
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    9462..9583
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(9632..9648)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9656..9672
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9680..9710)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9725..9746
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10034..10622)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10793..11653)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11654..11758)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatggg tgtcctcaaa
      721 gtgtggctcg ggctggccct agcgttggcg gaatttgcag tattgcctca tcattccgaa
      781 ggtgcttgtg tctatcagga ttccttgttg gcggatgcca caatttggaa gcccgattca
      841 tgccagagct gccgttgcca tggtgatatt gttatctgca aacctgctgt ttgcagaaac
      901 cctcaatgtg cctttgagaa gggagaagtg cttcaaatag ctgccaacca atgctgtcct
      961 gagtgtgttt tgaggactcc aggatcttgc catcatgaaa agaaaatcca tgagcatggg
     1021 acagaatggg cctcttctcc atgtagtgtg tgctcttgca atcatgggga agtccgatgt
     1081 accccccaac catgcccacc gctgtcatgt ggacaccagg agctggcatt catccctgaa
     1141 ggaagctgct gcccagtttg tgtgggcctt gggaaaccct gttcctatga aggccatgtg
     1201 tttcaggatg gggaggactg gcggctgagc cggtgtgcca aatgtctgtg tagaaatggg
     1261 gttgcccagt gcttcacagc tcagtgtcag cctctatttt gtaaccagga tgagactgta
     1321 gtccgagtcc ctggaaaatg ttgcccgcag tgctctgcaa gatcctgctc tgcagctggc
     1381 caagtatacg agcatggtga gcagtggagc gaaaatgcct gcaccacgtg tatatgtgac
     1441 cggggtgagg tcaggtgtca caagcaggcc tgcctgcccc tgagatgcgg aaagggtcag
     1501 agcagggctc ggcgtcatgg gcaatgctgt gaggaatgtg tgtctcctgc cgggagctgc
     1561 tcctatgatg gagttgtgcg gtaccaggac gaaatgtgga agggctcggc ctgtgagttc
     1621 tgcatgtgtg atcatggcca agtgacctgc cagactggag agtgtgccaa agtggagtgt
     1681 gcccgggatg aagaattaat tcacttagat ggaaagtgtt gtcctgaatg catttcaagg
     1741 aatggttatt gtgtttatga agaaactgga gaatttatgt catcaaatgc tagtgaagtt
     1801 aaacgtattc cagagggaga gaagtgggaa gatggccctt gcaaggtgtg tgagtgccga
     1861 ggggctcagg taacttgcta cgagccctct tgcccaccat gtccagtggg cacactggcc
     1921 ttagaggtga agggacagtg ctgtccagac tgcacatcag ttcattgcca tccagattgt
     1981 ttgacatgct ctcagtctcc agaccactgt gacctctgcc aagatcctac caagttactg
     2041 cagaatggat ggtgtgtgca cagctgtgga ctgggttttt accaagctgg cagtctctgt
     2101 ttagcctgcc agccccagtg ctccacgtgt accagtgggc tggagtgctc atcctgccag
     2161 cctcccctgc tgatgcggca cgggcagtgt gtgcctacct gtggggacgg cttctaccaa
     2221 gatcgccatt cctgtgcagt ctgccatgag tcctgtgcag gttgctgggg cccaacggag
     2281 aagcactgct tggcctgcag agatcccctc cacgtgctga gagatggcgg ctgtgagagc
     2341 agctgtggaa aaggcttcta caacaggcag ggcacctgta gcgcttgtga ccaatcctgt
     2401 gacagttgtg gccccagtag ccccaggtgt cttacctgta ctgagaagac agtgctgcat
     2461 gatgggaaat gcatgtctga atgccctggc gggtactatg ctgatgccac tggcaggtgc
     2521 aaagtttgtc ataactcatg tgccagctgc tctgggccca caccctctca ctgtacagcc
     2581 tgcagccccc ccaaggctct gcgtcaaggc cactgtctgc cccgctgtgg agagggtttc
     2641 tactctgacc atggagtctg caaagcctgt cactcctcct gcctggcttg tatgggtccc
     2701 gcaccctctc actgtactgg gtgtaagaag ccagaggaag gactgcaagt ggagcagctg
     2761 tctgacgtgg gcatcccctc tggcgagtgt ctagcccagt gtagagccca tttttacttg
     2821 gagagcactg gcatatgtga agcttgccac cagtcctgtt tcagatgtgc agggaaaagc
     2881 ccacataact gcacagactg tgggccttcc catgtgctgt tggatgggca gtgcctctcc
     2941 cagtgcccag atggctactt tcaccaggaa ggtagttgca cagagtgtca cccaacctgc
     3001 aggcagtgtc atgggccgtt ggagtctgac tgcatctcct gttaccctca catctctctt
     3061 accaatggta actgcaggac cagctgcagg gaagagcagt tcctcaacct cgtgggatac
     3121 tgtgctgact gccatcacct gtgccagcac tgtgcagctg atctccacaa cactgggagc
     3181 atctgcctca ggtgccagaa tgcccactac ctgctgctcg gggaccactg tgttcctgac
     3241 tgcccttcag gatactatgc agagagagga gcttgtaaaa aatgccactc ctcctgcaga
     3301 acctgccagg gcagaggacc tttctcctgc tcctcatgtg acaccaacct cgtgctgtcc
     3361 cacactggca cctgcagcac cacctgcttc cctgggcact atcttgatga caatcatgtt
     3421 tgccagccat gcaacacaca ctgtggaagc tgtgattcac aggccagctg tacctcctgc
     3481 cgagatccaa acaaggttct gctctttggg gaatgtcaat acgagagctg cgccccacag
     3541 tactatcttg acttctccac caacacgtgc aaagagtgtg attggagctg cagtgcatgc
     3601 agtgggcccc tgaaaacaga ctgcctgcag tgcatggatg gctatgttct ccaggatggg
     3661 gcctgcgtgg agcagtgctt gtcatcattt taccaggact cgggcctctg caagaactgt
     3721 gacagctact gtctccagtg ccaaggtccc catgagtgta cccgctgcaa agggccattt
     3781 ctcctcttgg aagcccagtg tgtccaggaa tgtgggaagg ggtactttgc agatcatgca
     3841 aagcacaaat gcacagcctg ccctcagggg tgcttgcagt gcagccacag ggaccgttgt
     3901 cacctctgtg accatgggtt ctttctgaag agtggcctct gtgtttacaa ctgtgttcct
     3961 ggcttttctg tccacacctc taatgaaaca tgttctggca aaatacacac ccctagtctt
     4021 catgtgaatg gttccctgat cctcccaatt ggttcaataa agccactgga tttttccctc
     4081 ctgaatgtcc aagaccagga gggtagggtc gaagatctcc tatttcatgt tgtgagcact
     4141 cccaccaatg gtcagctagt gctctcaaga aatggaaaag aggttcagct ggacaaggct
     4201 ggccgtttta gctggaaaga tgtgaacgag aagaaagtgc gttttgtgca cagcaaagaa
     4261 aaactcagga aaggttacct tttcctgaaa attagtgacc agcagttctt ctctgagcca
     4321 cagctgatca acatacaagc attttcaaca caggccccct atgtgctgag aaatgaagtt
     4381 ctccacatta gcagaggaga gagggcaacc atcaccaccc agatgcttga catccgagat
     4441 gatgacaacc cacaggatgt ggtcattgaa ataatcgatc ctccacttca tggccaattg
     4501 cttcagacac ttcagtcccc ggcaacccct atctatcaat tccagctgga tgaactctct
     4561 agaggccttc tccactatgc tcatgatggt tcagacagca catccgatgt tgcagtcttg
     4621 caggccaatg atggacactc cttccataat atactgttcc aagtgaagac cgtgcctcag
     4681 aatgacaggg gtcttcagct tgtggctaat tcgatggtgt gggttccaga aggggggatg
     4741 ctgcagatca ccaacagaat cttacaggcc gaggctcctg gtgccagtgc tgaagaaatc
     4801 atctacaaga ttacacaaga ctacccccag tttggggagg tggtccttct agtgaatatg
     4861 cctgcagaca gccctgcaga tgaagggcag cacctgcctg atgggaggac agctaccccc
     4921 accagcacct tcacccagca ggacatcaat gaaggcatcg tatggtacag gcactcagga
     4981 gccccagccc agagcgactc cttccgcttc gaggtgtcca gtgcctccaa tgcccagacc
     5041 cgcctggaga gccacatgtt caacatcgcg atcttaccac agacacctga agcacctaaa
     5101 gtgtctctgg aagcatctct ccatatgact gctagagaag atggcctgac tgttattcag
     5161 cctcattccc tctccttcat aaactctgag aagccaagtg gaaagattgt ctacaacatc
     5221 actctacctc tgcatccaaa tcaaggtatc atcgagcacc gggaccaccc tcactctcct
     5281 atccggtatt tcacgcaaga ggatattaac cagggcaaag tcatgtaccg ccctcccccg
     5341 gcagcacccc acctccagga gctcatggcc ttctcgttcg ctggtctccc agaatcagtg
     5401 aaattccact tcacagtttc agatggagaa cacacaagtc cggagatggt cctcaccatt
     5461 cacttacttc ccagtgatca gcaactgcca gtgttccagg tcacagctcc acggctggcg
     5521 gtcagcccag gaggcagcac ttctgtagga cttcaggtgg tagtaagaga tgctgagaca
     5581 gcgcccaaag aactcttctt tgagcttcgg agacctccac agcatggtgt gcttcttaag
     5641 catacagctg agttccgaag gccgatggcc acaggtgaca ctttcaccta tgaggatgtt
     5701 gagaaaaatg ctctacagta tatacatgat ggttcctcta cccgggaaga cagcatggag
     5761 atctcagtca cagatggcct cacagtgaca atgctggagg tgagagtaga ggtgtccctg
     5821 tcagaagacc gagggcctcg actggctgct ggctcctctc tgagcattac tgttgccagt
     5881 aaaagcacag ccataatcac taggtcacac cttgcttacg tggatgattc ttcccccgac
     5941 ccagagatct ggattcagtt aaattatctg ccctcatatg gtactctctt aagaatctca
     6001 ggatctgagg tggaagagct ctcagaagtt tccaatttca caatggaaga catcaataac
     6061 aagaaaatca gatactcagc tgtgtttgaa actgatggtc atctggttac tgatagcttc
     6121 tatttctctg tctctgacat ggaccacaac catctggata atcagatatt taccatcatg
     6181 atcactcctg ctgaaaatcc acctccagtc attgcttttg ctgaccttat cacggttgat
     6241 gagggaggga gagcaccact ctcatttcac catttttttg ctactgatga tgatgacaac
     6301 ctccagagag atgccatcat taaactaagt gctctgccca aatatggctg cattgagaac
     6361 acaggaacag gtgatcgttt tggccctgaa actgccagtg acctagaggc atcatttcct
     6421 attcaagacg tcctggaaaa ctacatttac tactttcaga gtgttcatga aagcattgag
     6481 ccaacccatg atatttttag tttttatgtg agtgatggaa ccagtcgttc agaaattcac
     6541 agcatcaata tcaccattga ggtaaagact ttggaagttg gaaaggttga gcccttgacc
     6601 acaatatttc acaccattag agagctttct ttactcgagt acccatacga cgtacctgat
     6661 tatgcaactt atccttatga tgtacctgat tatgctagct atccttatga tgtcccagac
     6721 tacgcatagt gagggcccga tatctcgaca atcaacctct ggattacaaa atttgtgaaa
     6781 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     6841 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     6901 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     6961 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     7021 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     7081 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     7141 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     7201 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     7261 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     7321 tttgggccgc ctccccgcct ggaaacgggg gaggctaact gaaacacgga aggagacaat
     7381 accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acgggtgttg
     7441 ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac
     7501 cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa
     7561 gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcag
     7621 atctgcgcag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     7681 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     7741 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     7801 taaatcgggg catcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     7861 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     7921 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     7981 tcaaccctat ctcggtctat tcttttgatt tataagggat tttggggatt tcggcctatt
     8041 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     8101 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     8161 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     8221 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     8281 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     8341 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     8401 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     8461 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     8521 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     8581 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     8641 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
     8701 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     8761 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     8821 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     8881 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     8941 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     9001 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     9061 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     9121 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     9181 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     9241 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     9301 ttcgcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     9361 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     9421 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     9481 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     9541 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     9601 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     9661 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     9721 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     9781 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     9841 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     9901 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     9961 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
    10021 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
    10081 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
    10141 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
    10201 tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt
    10261 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
    10321 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
    10381 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
    10441 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
    10501 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
    10561 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
    10621 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
    10681 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
    10741 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
    10801 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
    10861 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
    10921 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
    10981 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
    11041 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
    11101 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
    11161 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
    11221 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
    11281 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    11341 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    11401 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    11461 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    11521 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    11581 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    11641 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    11701 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    11761 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
    11821 gtcgactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
    11881 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
    11941 cttgaccgac aattgcatga agaatctgct tagg
//
