LOCUS       V018799                 5852 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V018799
VERSION     V018799
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             998..2014
                     /label="Fam50a(NM_138607)"
                     /note="Fam50a(NM_138607)"
                     /gene="Fam50a"
     CDS             2018..2047
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2066..2089
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2109..2125)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2136..2758
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(2907..3495)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    3824..3871
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4103..4897)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(4932..5289)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5291..5395)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5422..5850
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccatg gcccagtaca agggcgctgc
     1021 aagcgaggcg ggacgcgcca tgcacctaat gaagaagagg gagaagcagc gtgagcaaat
     1081 ggagcagatg aagcagagga ttgcagagga gaacataatg aaatctaaca ttgacaagaa
     1141 attctctgca cactatgatg ctgtggaagc agagctcaag tccagtactg tgggtcttgt
     1201 gactctgaat gacatgaagg ccaaacagga ggcactggtg aaggagcgag agaaacagct
     1261 ggcaaaaaag gagcagtcaa aggagctaca gctgaaacta gagaagcttc gagagaagga
     1321 gcgcaagaag gaggccaagc ggaagatctc cagcctgtcc ttcaccttgg aggaagagga
     1381 ggagggagtt gaagaggagg aagagatggc tatgtacgag gaggaactgg agagggaaga
     1441 gatcaccaca aagaaaaaga aattggggaa gaaccctgat gtggacacaa gcttcttgcc
     1501 tgaccgggac cgggaggagg aggagaatcg gcttcgggaa gagctgcgcc aggagtggga
     1561 agccaagcag gaaaaaatca agagtgaaga gattgaaatc acctttagtt actgggatgg
     1621 ctctgggcac cggcgcacag ttaagatgaa gaaaggcaac acgatgcagc agttcctaca
     1681 gaaagcacta gagatcttgc ggaaagactt cagtgaactc aggtcagcag gggtggaaca
     1741 gctcatgtac atcaaggagg atttaatcat cccccatcat cacagcttct atgacttcat
     1801 cgttaccaag gcacgaggaa aaagtgggcc actcttcaac ttcgacgttc atgatgatgt
     1861 acgactgctc agtgatgcca ctgtggagaa ggatgagtca catgcaggca aagtagtgct
     1921 aaggagctgg tatgagaaga acaagcacat ttttcctgcc agtcgctggg agccctatga
     1981 cccagaaaag aaatgggaca agtacacgat ccggctcgag cagaaactca tctcagaaga
     2041 ggatctggca gcaaatgata tcctggatta caaggatgac gacgataagg tttaaacggc
     2101 cggccgcggt catagctgtt tcctgaacag atcccgggtg gcatccctgt gacccctccc
     2161 cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt gtcctaataa
     2221 aattaagttg catcattttg tctgactagg tgtccttcta taatattatg gggtggaggg
     2281 gggtggtatg gagcaagggg caagttggga agacaacctg tagggcctgc ggggtctatt
     2341 gggaaccaag ctggagtgca gtggcacaat cttggctcac tgcaatctcc gcctcctggg
     2401 ttcaagcgat tctcctgcct cagcctcccg agttgttggg attccaggca tgcatgacca
     2461 ggctcagcta atttttgttt ttttggtaga gacggggttt caccatattg gccaggctgg
     2521 tctccaactc ctaatctcag gtgatctacc caccttggcc tcccaaattg ctgggattac
     2581 aggcgtgaac cactgctccc ttccctgtcc ttctgatttt aaaataacta taccagcagg
     2641 aggacgtcca gacacagcat aggctacctg gccatgccca accggtggga catttgagtt
     2701 gcttgcttgg cactgtcctc tcatgcgttg ggtccactca gtagatgcct gttgaattgg
     2761 gtacgcggcc agcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     2821 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     2881 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     2941 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     3001 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     3061 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     3121 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     3181 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     3241 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     3301 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     3361 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     3421 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     3481 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     3541 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     3601 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta acctgaggct
     3661 atggcagggc ctgccgcccc gacgttggct gcgagccctg ggccttcacc cgaacttggg
     3721 gggtggggtg gggaaaagga agaaacgcgg gcgtattggc cccaatgggg tctcggtggg
     3781 gtatcgacag agtgccagcc ctgggaccga accccgcgtt tatgaacaaa cgacccaaca
     3841 ccgtgcgttt tattctgtct ttttattgcc gtcatagcgc gggttccttc cggtattgtc
     3901 tccttccgtg tttcagttag cctcccccta gggtgggcga agaactccag catgagatcc
     3961 ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc caacctttca
     4021 tagaaggcgg cggtggaatc gaaatctcgt gatggcaggt tgggcgtcgc ttggtcggtc
     4081 atttcgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag aaggcgatgc
     4141 gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc
     4201 caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcga tccgccacac
     4261 ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca
     4321 agcaggcatc gccatgggtc acgacgagat cctcgccgtc gggcatgctc gccttgagcc
     4381 tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca tcctgatcga
     4441 caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga
     4501 atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata
     4561 ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact tcgcccaata
     4621 gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg
     4681 tcgtggccag ccacgatagc cgcgctgcct cgtcttgcag ttcattcagg gcaccggaca
     4741 ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac acggcggcat
     4801 cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagcgg
     4861 ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct catcctgtct
     4921 cttgatcgat ctttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta cttctggaat
     4981 agctcagagg ccgaggcggc ctcggcctct gcataaataa aaaaaattag tcagccatgg
     5041 ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt taggggcggg
     5101 actatggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct
     5161 ggggactttc cacacctggt tgctgactaa ttgagatgca tgctttgcat acttctgcct
     5221 gctggggagc ctggggactt tccacaccct aactgacaca cattccacag ctggttcttt
     5281 ccgcctcagg actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     5341 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     5401 ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     5461 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     5521 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     5581 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     5641 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     5701 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     5761 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     5821 agctgattta acaaaaattt aacgcgaatt tt
//
