LOCUS       V029675                 6219 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029675
VERSION     V029675
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2366
                     /label="Foxf2(NM_010225)"
                     /note="Foxf2(NM_010225)"
                     /gene="Foxf2"
     CDS             2385..2414
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2433..2456
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2476..2492)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2503..3125
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3274..3862)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4191..4238
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4470..5264)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5299..5656)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5658..5762)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5789..6217
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gagcaccgag ggcgggcctc cgccaccccc gccgcgcccg ccgcctgccc
     1081 cactccgccg cgcgtgcagc ccggcgcccg gcgcgctcca ggccgccttg atgagcccgc
     1141 cacccgccgc caccctggag tccacttcgt cgtcgtcatc atcatcctct gcctcctgtg
     1201 cctcgtcctc ttctaactcc gtcagcgcct cggccggtgc ttgcaagagt gcggctagca
     1261 gcggcggcgc gggcgccggg agtggaggca ccaagaaggc aacctcgggg ctgcggcggc
     1321 cggagaagcc tccttactcg tacatcgcgc tcatcgtcat ggccatccag agctcgccca
     1381 gcaagcgcct gacgctcagt gagatctacc agttcctaca ggcgcgcttc ccctttttcc
     1441 gtggcgccta ccagggctgg aagaactccg tgcgccacaa cctctcgctc aacgagtgct
     1501 tcatcaagct gcccaagggc ctcgggagac ctggtaaggg ccactactgg accatcgacc
     1561 cggccagcga attcatgttt gaggagggtt cgttccgccg ccggccgcgc ggcttcaggc
     1621 ggaagtgcca ggctctcaaa cccatgtacc atcgcgtggt gagcggcttg ggcttcgggg
     1681 cctcgctgct gccccagggc ttcgacttcc aagcgccccc gtcggcgcct ctgggttgcc
     1741 acggtcaagg cggttatggt ggcctcgaca tgatgcccgc gggctatgat acaggggcgg
     1801 gtgctccggg ccacgcgcat ccacaccacc tccaccacca ccacgtcccc cacatgtcgc
     1861 ccaacccggg ctccacctat atggccagct gcccggtgcc cgcaggtcct gcgggcgtcg
     1921 gtgcggcagc gggtggcggc ggtggcggcg gggactatgg gccggacagc agcagcagcc
     1981 ctgtgccctc atccccggct atggcaagcg ccattgagtg tcactcgccc tacactagcc
     2041 ctgcggcaca ttggagctcg cctggcgctt caccttacct caagcagccg cctgccctga
     2101 cgccaagcag taatcccgcg gcctctgctg gtctgcaccc cagcatgtct tcctactcgt
     2161 tggagcagag ctacttgcac cagaacgccc gcgaggatct ctcagtcgga ctgccccgtt
     2221 accagcacca ctccactcca gtgtgcgaca ggaaagattt cgtcctcaat ttcaatggca
     2281 tttcttcttt ccacccttcc gctagtggct cttattatca ccatcatcac cagagcgtgt
     2341 gccaagatat taagccctgt gttatgacgc gtacgcggcc gctcgagcag aaactcatct
     2401 cagaagagga tctggcagca aatgatatcc tggattacaa ggatgacgac gataaggttt
     2461 aaacggccgg ccgcggtcat agctgtttcc tgaacagatc ccgggtggca tccctgtgac
     2521 ccctccccag tgcctctcct ggccctggaa gttgccactc cagtgcccac cagccttgtc
     2581 ctaataaaat taagttgcat cattttgtct gactaggtgt ccttctataa tattatgggg
     2641 tggagggggg tggtatggag caaggggcaa gttgggaaga caacctgtag ggcctgcggg
     2701 gtctattggg aaccaagctg gagtgcagtg gcacaatctt ggctcactgc aatctccgcc
     2761 tcctgggttc aagcgattct cctgcctcag cctcccgagt tgttgggatt ccaggcatgc
     2821 atgaccaggc tcagctaatt tttgtttttt tggtagagac ggggtttcac catattggcc
     2881 aggctggtct ccaactccta atctcaggtg atctacccac cttggcctcc caaattgctg
     2941 ggattacagg cgtgaaccac tgctcccttc cctgtccttc tgattttaaa ataactatac
     3001 cagcaggagg acgtccagac acagcatagg ctacctggcc atgcccaacc ggtgggacat
     3061 ttgagttgct tgcttggcac tgtcctctca tgcgttgggt ccactcagta gatgcctgtt
     3121 gaattgggta cgcggccagc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     3181 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     3241 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     3301 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     3361 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     3421 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     3481 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     3541 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     3601 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     3661 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     3721 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     3781 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     3841 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     3901 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     3961 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaacc
     4021 tgaggctatg gcagggcctg ccgccccgac gttggctgcg agccctgggc cttcacccga
     4081 acttgggggg tggggtgggg aaaaggaaga aacgcgggcg tattggcccc aatggggtct
     4141 cggtggggta tcgacagagt gccagccctg ggaccgaacc ccgcgtttat gaacaaacga
     4201 cccaacaccg tgcgttttat tctgtctttt tattgccgtc atagcgcggg ttccttccgg
     4261 tattgtctcc ttccgtgttt cagttagcct ccccctaggg tgggcgaaga actccagcat
     4321 gagatccccg cgctggagga tcatccagcc ggcgtcccgg aaaacgattc cgaagcccaa
     4381 cctttcatag aaggcggcgg tggaatcgaa atctcgtgat ggcaggttgg gcgtcgcttg
     4441 gtcggtcatt tcgaacccca gagtcccgct cagaagaact cgtcaagaag gcgatagaag
     4501 gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat
     4561 tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcgatcc
     4621 gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata
     4681 ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catgctcgcc
     4741 ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc
     4801 tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg
     4861 tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg
     4921 atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg
     4981 cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga
     5041 acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cttgcagttc attcagggca
     5101 ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg
     5161 gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc
     5221 caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat
     5281 cctgtctctt gatcgatctt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt
     5341 ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca
     5401 gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag
     5461 gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
     5521 ggagcctggg gactttccac acctggttgc tgactaattg agatgcatgc tttgcatact
     5581 tctgcctgct ggggagcctg gggactttcc acaccctaac tgacacacat tccacagctg
     5641 gttctttccg cctcaggact cttccttttt caatattatt gaagcattta tcagggttat
     5701 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     5761 cgcacatttc cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg
     5821 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     5881 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     5941 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     6001 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     6061 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     6121 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     6181 aaaaatgagc tgatttaaca aaaatttaac gcgaatttt
//
