LOCUS       V039232                 8710 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039232
VERSION     V039232
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..3369
                     /label="HMGCR(NM_000859)"
                     /note="HMGCR(NM_000859)"
                     /gene="HMGCR"
     CDS             3376..3402
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3406..3432
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3436..3462
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     misc_feature    3486..4074
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    4140..4188
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      4390..4818
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4832..5161
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5228..6022
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6198..6319
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6368..6384)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6392..6408
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6416..6446)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6461..6482
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6770..7358)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7529..8389)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8390..8494)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgtt gtcaagactt
      721 tttcgaatgc atggcctctt tgtggcctcc catccctggg aagtcatagt ggggacagtg
      781 acactgacca tctgcatgat gtccatgaac atgtttactg gtaacaataa gatctgtggt
      841 tggaattatg aatgtccaaa gtttgaagag gatgttttga gcagtgacat tataattctg
      901 acaataacac gatgcatagc catcctgtat atttacttcc agttccagaa tttacgtcaa
      961 cttggatcaa aatatatttt gggtattgct ggccttttca caattttctc aagttttgta
     1021 ttcagtacag ttgtcattca cttcttagac aaagaattga caggcttgaa tgaagctttg
     1081 ccctttttcc tacttttgat tgacctttcc agagcaagca cattagcaaa gtttgccctc
     1141 agttccaact cacaggatga agtaagggaa aatattgctc gtggaatggc aattttaggt
     1201 cctacgttta ccctcgatgc tcttgttgaa tgtcttgtga ttggagttgg taccatgtca
     1261 ggggtacgtc agcttgaaat tatgtgctgc tttggctgca tgtcagttct tgccaactac
     1321 ttcgtgttca tgactttctt cccagcttgt gtgtccttgg tattagagct ttctcgggaa
     1381 agccgcgagg gtcgtccaat ttggcagctc agccattttg cccgagtttt agaagaagaa
     1441 gaaaataagc cgaatcctgt aactcagagg gtcaagatga ttatgtctct aggcttggtt
     1501 cttgttcatg ctcacagtcg ctggatagct gatccttctc ctcaaaacag tacagcagat
     1561 acttctaagg tttcattagg actggatgaa aatgtgtcca agagaattga accaagtgtt
     1621 tccctctggc agttttatct ctctaaaatg atcagcatgg atattgaaca agttattacc
     1681 ctaagtttag ctctccttct ggctgtcaag tacatcttct ttgaacaaac agagacagaa
     1741 tctacactct cattaaaaaa ccctatcaca tctcctgtag tgacacaaaa gaaagtccca
     1801 gacaattgtt gtagacgtga acctatgctg gtcagaaata accagaaatg tgattcagta
     1861 gaggaagaga cagggataaa ccgagaaaga aaagttgagg ttataaaacc cttagtggct
     1921 gaaacagata ccccaaacag agctacattt gtggttggta actcctcctt actcgatact
     1981 tcatcagtac tggtgacaca ggaacctgaa attgaacttc ccagggaacc tcggcctaat
     2041 gaagaatgtc tacagatact tgggaatgca gagaaaggtg caaaattcct tagtgatgct
     2101 gagatcatcc agttagtcaa tgctaagcat atcccagcct acaagttgga aactctgatg
     2161 gaaactcatg agcgtggtgt atctattcgc cgacagttac tttccaagaa gctttcagaa
     2221 ccttcttctc tccagtacct accttacagg gattataatt actccttggt gatgggagct
     2281 tgttgtgaga atgttattgg atatatgccc atccctgttg gagtggcagg acccctttgc
     2341 ttagatgaaa aagaatttca ggttccaatg gcaacaacag aaggttgtct tgtggccagc
     2401 accaatagag gctgcagagc aataggtctt ggtggaggtg ccagcagccg agtccttgca
     2461 gatgggatga ctcgtggccc agttgtgcgt cttccacgtg cttgtgactc tgcagaagtg
     2521 aaagcctggc tcgaaacatc tgaagggttc gcagtgataa aggaggcatt tgacagcact
     2581 agcagatttg cacgtctaca gaaacttcat acaagtatag ctggacgcaa cctttatatc
     2641 cgtttccagt ccaggtcagg ggatgccatg gggatgaaca tgatttcaaa gggtacagag
     2701 aaagcacttt caaaacttca cgagtatttc cctgaaatgc agattctagc cgttagtggt
     2761 aactattgta ctgacaagaa acctgctgct ataaattgga tagagggaag aggaaaatct
     2821 gttgtttgtg aagctgtcat tccagccaag gttgtcagag aagtattaaa gactaccaca
     2881 gaggctatga ttgaggtcaa cattaacaag aatttagtgg gctctgccat ggctgggagc
     2941 ataggaggct acaacgccca tgcagcaaac attgtcaccg ccatctacat tgcctgtgga
     3001 caggatgcag cacagaatgt tggtagttca aactgtatta ctttaatgga agcaagtggt
     3061 cccacaaatg aagatttata tatcagctgc accatgccat ctatagagat aggaacggtg
     3121 ggtggtggga ccaacctact acctcagcaa gcctgtttgc agatgctagg tgttcaagga
     3181 gcatgcaaag ataatcctgg ggaaaatgcc cggcagcttg cccgaattgt gtgtgggacc
     3241 gtaatggctg gggaattgtc acttatggca gcattggcag caggacatct tgtcaaaagt
     3301 cacatgattc acaacaggtc gaagatcaat ttacaagacc tccaaggagc ttgcaccaag
     3361 aagacagccc tcgagtaccc atacgacgta cctgattatg caacttatcc ttatgatgta
     3421 cctgattatg ctagctatcc ttatgatgtc ccagactacg catagtgagg gcccgatatc
     3481 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     3541 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     3601 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     3661 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     3721 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     3781 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     3841 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     3901 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     3961 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     4021 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa
     4081 acgggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac
     4141 ggcaataaaa agacagaata aaacgcacgg gtgttgggtc gtttgttcat aaacgcgggg
     4201 ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg
     4261 cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg
     4321 cagccaacgt cggggcggca ggccctgcca tagcagatct gcgcagctgg ggctctaggg
     4381 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     4441 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     4501 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcggggcatc cctttagggt
     4561 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     4621 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     4681 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     4741 ttgatttata agggattttg gggatttcgg cctattggtt aaaaaatgag ctgatttaac
     4801 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     4861 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     4921 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     4981 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     5041 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     5101 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     5161 aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc
     5221 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     5281 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     5341 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     5401 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     5461 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     5521 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     5581 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     5641 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     5701 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
     5761 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     5821 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     5881 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     5941 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     6001 ccttcttgac gagttcttct gagcgggact ctggggttcg cgaaatgacc gaccaagcga
     6061 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     6121 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     6181 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     6241 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     6301 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     6361 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     6421 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     6481 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     6541 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     6601 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     6661 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     6721 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6781 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6841 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6901 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6961 ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     7021 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     7081 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     7141 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     7201 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     7261 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     7321 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     7381 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     7441 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     7501 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     7561 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     7621 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     7681 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     7741 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     7801 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     7861 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     7921 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     7981 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     8041 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     8101 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     8161 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     8221 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     8281 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     8341 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     8401 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     8461 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     8521 acgtcgacgg atcgggagat ctcccgatcc cctatggtcg actctcagta caatctgctc
     8581 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
     8641 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
     8701 tctgcttagg
//
