LOCUS V027150 8239 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V027150 VERSION V027150 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 706..2895 /label="HNRNPM(NM_005968)" /note="HNRNPM(NM_005968)" /gene="HNRNPM" CDS 2902..2991 /codon_start=1 /product="3 tandem Myc epitope tags" /transl_table=1 /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL" /label="3xMyc" misc_feature 3015..3603 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 3669..3717 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 3919..4347 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4361..4690 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4757..5551 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5727..5848 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5897..5913) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5921..5937 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5945..5975) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5990..6011 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6299..6887) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7058..7918) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7919..8023) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg 661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatggc ggcaggggtc 721 gaagcggcgg cggaggtggc ggcgacggag atcaaaatgg aggaagagag cggcgcgccc 781 ggcgtgccga gcggcaacgg ggctccgggc cctaagggtg aaggagaacg acctgctcag 841 aatgagaaga ggaaggagaa aaacataaaa agaggaggca atcgctttga gccatatgcc 901 aatccaacta aaagatacag agccttcatt acaaacatac cttttgatgt gaaatggcag 961 tcacttaaag acctggttaa agaaaaagtt ggtgaggtaa catacgtgga gctcttaatg 1021 gacgctgaag gaaagtcaag gggatgtgct gttgttgaat tcaagatgga agagagcatg 1081 aaaaaagctg cggaagtcct aaacaagcat agtctgagcg gaagaccact gaaagtcaaa 1141 gaagatcctg atggtgaaca tgccaggaga gcaatgcaaa aggtgatggc tacgactggt 1201 gggatgggta tgggaccagg tggcccagga atgattacta tcccacccag tatcctaaat 1261 aatcccaaca tcccaaatga gattatccat gcattacagg ctggaagact tggaagcaca 1321 gtatttgtag caaatctgga ttataaagtt ggctggaaga aactgaagga agtatttagt 1381 atggctggtg tggtggtccg agcagacatt cttgaagata aagatggaaa aagtcgtgga 1441 ataggcactg ttacttttga acagtccatt gaagctgtgc aagctatatc tatgttcaat 1501 ggccagctgc tatttgatag accaatgcac gtcaagatgg atgagagggc cttaccaaaa 1561 ggagatttct tccctcctga gcgtccacaa caacttcccc atggccttgg tggtattggc 1621 atggggttag gaccaggagg gcaacccatt gatgccaatc acctgaataa aggcatcgga 1681 atgggaaaca taggtcccgc aggaatggga atggaaggca taggatttgg aataaataaa 1741 atgggaggaa tggaggggcc ctttggtggt ggtatggaaa acatgggtcg atttggatct 1801 gggatgaaca tgggcaggat aaatgaaatc ctaagtaatg cactgaagag aggagagatc 1861 attgcaaagc agggaggagg tggaggtgga ggaagcgtcc ctgggatcga gaggatgggt 1921 cctggcattg accgcctcgg gggtgccggc atggagcgca tgggcgcggg cctgggccac 1981 ggcatggatc gcgtgggctc cgagatcgag cgcatgggcc tggtcatgga ccgcatgggc 2041 tccgtggagc gcatgggctc cggcattgag cgcatgggcc cgctgggcct cgaccacatg 2101 gcctccagca ttgagcgcat gggccagacc atggagcgca ttggctctgg cgtggagcgc 2161 atgggtgccg gcatgggctt cggccttgag cgcatggccg ctcccatcga ccgtgtgggc 2221 cagaccattg agcgcatggg ctctggcgtg gagcgcatgg gccctgccat cgagcgcatg 2281 ggcctgagca tggagcgcat ggtgcccgca ggtatgggag ctggcctgga gcgcatgggc 2341 cccgtgatgg atcgcatggc caccggcctg gagcgcatgg gcgccaacaa tctggagcgg 2401 atgggcctgg agcgcatggg cgccaacagc ctcgagcgca tgggcctgga gcgcatgggt 2461 gccaacagcc tcgagcgcat gggccccgcc atgggcccgg ccctgggcgc tggcattgag 2521 cgcatgggcc tggccatggg tggcggtggc ggtgccagct ttgaccgtgc catcgagatg 2581 gagcgtggca acttcggagg aagcttcgca ggttcctttg gtggagctgg aggccatgct 2641 cctggggtgg ccaggaaggc ctgccagata tttgtgagaa atctgccatt cgatttcaca 2701 tggaagatgc taaaggacaa attcaacgag tgcggccacg tgctgtacgc cgacatcaag 2761 atggagaatg ggaagtccaa ggggtgtggc gtggttaagt tcgagtcgcc agaggtggcc 2821 gagagagcct gccggatgat gaatggcatg aagctgagtg gccgagagat tgacgttcga 2881 attgatagaa acgctctcga ggaacaaaaa ctaatatcag aggaagattt ggaacagaaa 2941 ctaatcagtg aagaagattt agagcaaaag ttaatttccg aggaggactt atagtgaggg 3001 cccgatatct cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc 3061 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg 3121 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc 3181 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg 3241 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg 3301 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga 3361 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct 3421 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg 3481 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc 3541 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc 3601 cgcctggaaa cgggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg 3661 cgctatgacg gcaataaaaa gacagaataa aacgcacggg tgttgggtcg tttgttcata 3721 aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg 3781 gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc 3841 cagggctcgc agccaacgtc ggggcggcag gccctgccat agcagatctg cgcagctggg 3901 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg 3961 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct 4021 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cggggcatcc 4081 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg 4141 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt 4201 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg 4261 tctattcttt tgatttataa gggattttgg ggatttcggc ctattggtta aaaaatgagc 4321 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg 4381 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc 4441 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct 4501 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc 4561 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga 4621 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg 4681 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg 4741 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg 4801 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc 4861 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg 4921 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt 4981 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg 5041 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat 5101 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca 5161 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca 5221 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa 5281 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa 5341 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc 5401 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga 5461 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc 5521 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcgc gaaatgaccg 5581 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa 5641 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc 5701 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat 5761 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg 5821 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga 5881 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 5941 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 6001 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 6061 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 6121 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 6181 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 6241 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 6301 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 6361 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 6421 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 6481 tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 6541 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 6601 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 6661 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 6721 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 6781 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 6841 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 6901 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 6961 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 7021 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 7081 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 7141 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 7201 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 7261 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 7321 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 7381 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 7441 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 7501 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 7561 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 7621 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 7681 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 7741 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 7801 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 7861 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 7921 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 7981 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 8041 tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtcga ctctcagtac 8101 aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt 8161 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg 8221 catgaagaat ctgcttagg //