LOCUS       V027150                 8239 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V027150
VERSION     V027150
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..2895
                     /label="HNRNPM(NM_005968)"
                     /note="HNRNPM(NM_005968)"
                     /gene="HNRNPM"
     CDS             2902..2991
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     misc_feature    3015..3603
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    3669..3717
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      3919..4347
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4361..4690
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4757..5551
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5727..5848
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5897..5913)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5921..5937
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5945..5975)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5990..6011
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6299..6887)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7058..7918)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7919..8023)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatggc ggcaggggtc
      721 gaagcggcgg cggaggtggc ggcgacggag atcaaaatgg aggaagagag cggcgcgccc
      781 ggcgtgccga gcggcaacgg ggctccgggc cctaagggtg aaggagaacg acctgctcag
      841 aatgagaaga ggaaggagaa aaacataaaa agaggaggca atcgctttga gccatatgcc
      901 aatccaacta aaagatacag agccttcatt acaaacatac cttttgatgt gaaatggcag
      961 tcacttaaag acctggttaa agaaaaagtt ggtgaggtaa catacgtgga gctcttaatg
     1021 gacgctgaag gaaagtcaag gggatgtgct gttgttgaat tcaagatgga agagagcatg
     1081 aaaaaagctg cggaagtcct aaacaagcat agtctgagcg gaagaccact gaaagtcaaa
     1141 gaagatcctg atggtgaaca tgccaggaga gcaatgcaaa aggtgatggc tacgactggt
     1201 gggatgggta tgggaccagg tggcccagga atgattacta tcccacccag tatcctaaat
     1261 aatcccaaca tcccaaatga gattatccat gcattacagg ctggaagact tggaagcaca
     1321 gtatttgtag caaatctgga ttataaagtt ggctggaaga aactgaagga agtatttagt
     1381 atggctggtg tggtggtccg agcagacatt cttgaagata aagatggaaa aagtcgtgga
     1441 ataggcactg ttacttttga acagtccatt gaagctgtgc aagctatatc tatgttcaat
     1501 ggccagctgc tatttgatag accaatgcac gtcaagatgg atgagagggc cttaccaaaa
     1561 ggagatttct tccctcctga gcgtccacaa caacttcccc atggccttgg tggtattggc
     1621 atggggttag gaccaggagg gcaacccatt gatgccaatc acctgaataa aggcatcgga
     1681 atgggaaaca taggtcccgc aggaatggga atggaaggca taggatttgg aataaataaa
     1741 atgggaggaa tggaggggcc ctttggtggt ggtatggaaa acatgggtcg atttggatct
     1801 gggatgaaca tgggcaggat aaatgaaatc ctaagtaatg cactgaagag aggagagatc
     1861 attgcaaagc agggaggagg tggaggtgga ggaagcgtcc ctgggatcga gaggatgggt
     1921 cctggcattg accgcctcgg gggtgccggc atggagcgca tgggcgcggg cctgggccac
     1981 ggcatggatc gcgtgggctc cgagatcgag cgcatgggcc tggtcatgga ccgcatgggc
     2041 tccgtggagc gcatgggctc cggcattgag cgcatgggcc cgctgggcct cgaccacatg
     2101 gcctccagca ttgagcgcat gggccagacc atggagcgca ttggctctgg cgtggagcgc
     2161 atgggtgccg gcatgggctt cggccttgag cgcatggccg ctcccatcga ccgtgtgggc
     2221 cagaccattg agcgcatggg ctctggcgtg gagcgcatgg gccctgccat cgagcgcatg
     2281 ggcctgagca tggagcgcat ggtgcccgca ggtatgggag ctggcctgga gcgcatgggc
     2341 cccgtgatgg atcgcatggc caccggcctg gagcgcatgg gcgccaacaa tctggagcgg
     2401 atgggcctgg agcgcatggg cgccaacagc ctcgagcgca tgggcctgga gcgcatgggt
     2461 gccaacagcc tcgagcgcat gggccccgcc atgggcccgg ccctgggcgc tggcattgag
     2521 cgcatgggcc tggccatggg tggcggtggc ggtgccagct ttgaccgtgc catcgagatg
     2581 gagcgtggca acttcggagg aagcttcgca ggttcctttg gtggagctgg aggccatgct
     2641 cctggggtgg ccaggaaggc ctgccagata tttgtgagaa atctgccatt cgatttcaca
     2701 tggaagatgc taaaggacaa attcaacgag tgcggccacg tgctgtacgc cgacatcaag
     2761 atggagaatg ggaagtccaa ggggtgtggc gtggttaagt tcgagtcgcc agaggtggcc
     2821 gagagagcct gccggatgat gaatggcatg aagctgagtg gccgagagat tgacgttcga
     2881 attgatagaa acgctctcga ggaacaaaaa ctaatatcag aggaagattt ggaacagaaa
     2941 ctaatcagtg aagaagattt agagcaaaag ttaatttccg aggaggactt atagtgaggg
     3001 cccgatatct cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     3061 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     3121 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     3181 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     3241 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     3301 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     3361 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     3421 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     3481 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     3541 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     3601 cgcctggaaa cgggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg
     3661 cgctatgacg gcaataaaaa gacagaataa aacgcacggg tgttgggtcg tttgttcata
     3721 aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg
     3781 gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc
     3841 cagggctcgc agccaacgtc ggggcggcag gccctgccat agcagatctg cgcagctggg
     3901 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     3961 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     4021 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cggggcatcc
     4081 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     4141 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     4201 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     4261 tctattcttt tgatttataa gggattttgg ggatttcggc ctattggtta aaaaatgagc
     4321 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
     4381 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     4441 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     4501 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     4561 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     4621 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     4681 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg
     4741 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     4801 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     4861 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     4921 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     4981 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     5041 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     5101 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     5161 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     5221 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     5281 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     5341 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     5401 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     5461 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     5521 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcgc gaaatgaccg
     5581 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
     5641 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     5701 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
     5761 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     5821 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
     5881 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     5941 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     6001 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     6061 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     6121 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     6181 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     6241 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     6301 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     6361 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     6421 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     6481 tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     6541 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     6601 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     6661 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     6721 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     6781 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     6841 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     6901 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     6961 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     7021 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     7081 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     7141 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     7201 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     7261 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     7321 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     7381 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     7441 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     7501 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     7561 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     7621 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     7681 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     7741 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     7801 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     7861 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     7921 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     7981 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     8041 tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtcga ctctcagtac
     8101 aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt
     8161 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg
     8221 catgaagaat ctgcttagg
//
