LOCUS       V029500                 6165 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029500
VERSION     V029500
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2312
                     /label="Hdac3(NM_010411)"
                     /note="Hdac3(NM_010411)"
                     /gene="Hdac3"
     CDS             2331..2360
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2379..2402
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2422..2438)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2449..3071
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3220..3808)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4137..4184
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4416..5210)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5245..5602)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5604..5708)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5735..6163
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat ggccaagacc gtggcgtatt tctacgaccc cgatgtgggc aacttccact
     1081 atggagctgg acacccaatg aaacctcatc gcctggcatt gactcatagc ctagtcctgc
     1141 attatggtct ctataagaag atgatcgtct tcaagcctta ccaggcctcc cagcatgaca
     1201 tgtgccgctt ccattctgag gactacatcg acttcctgca gagagtcagc cccaccaata
     1261 tgcagggttt caccaagagc cttaatgcct tcaacgtggg tgatgactgc ccagtgtttc
     1321 caggactttt cgagttctgc tcccgttaca caggcgcatc tctgcaagga gcaacacagc
     1381 taaacaacaa gatctgtgat attgccatca actgggccgg tggtctacat catgccaaga
     1441 aatttgaggc ctctggcttc tgctatgtca atgacatagt aattggtatc ctggagctgc
     1501 ttaagtacca ccctcgggtg ctctacattg atatcgacat ccaccatggt gacggggttc
     1561 aggaagcctt ctacctcact gaccgggtca tgactgtgtc cttccacaaa tacggaaatt
     1621 acttctttcc tggaacaggt gacatgtatg aagttggagc agagagtggc cgctactatt
     1681 gtctcaatgt gcccttacga gatggcattg atgaccagag ttacaagcac cttttccagc
     1741 cagtcatcag ccaggtggtg gacttctacc agccgacgtg catcgtgctc cagtgtggcg
     1801 ctgactccct gggctgtgat cgattaggct gcttcaatct cagcattcga ggacatgggg
     1861 aatgtgttga atatgtcaag agtttcaata tccctctcct ggtactggga ggtggtggct
     1921 acactgtccg aaatgttgcc cggtgttgga catatgaaac atctctgctg gtagaagagg
     1981 ccattagtga ggaacttccc tatagtgaat acttcgagta ctttgcccca gatttcacac
     2041 tccatccaga tgtcagcacc cgcatcgaga atcagaactc acgccagtat ctggaccaga
     2101 tccgccagac aatctttgaa aacttgaaga tgctgaacca tgcacccagt gtccagattc
     2161 atgatgtccc ggcagacctc ctgacgtatg acaggactga cgaggccgac gctgaagaga
     2221 gaggtcccga ggagaactac agcaggccag aagcacccaa tgagttctat gatggcgacc
     2281 atgacaacga caaggaaagt gatgtggaga ttacgcgtac gcggccgctc gagcagaaac
     2341 tcatctcaga agaggatctg gcagcaaatg atatcctgga ttacaaggat gacgacgata
     2401 aggtttaaac ggccggccgc ggtcatagct gtttcctgaa cagatcccgg gtggcatccc
     2461 tgtgacccct ccccagtgcc tctcctggcc ctggaagttg ccactccagt gcccaccagc
     2521 cttgtcctaa taaaattaag ttgcatcatt ttgtctgact aggtgtcctt ctataatatt
     2581 atggggtgga ggggggtggt atggagcaag gggcaagttg ggaagacaac ctgtagggcc
     2641 tgcggggtct attgggaacc aagctggagt gcagtggcac aatcttggct cactgcaatc
     2701 tccgcctcct gggttcaagc gattctcctg cctcagcctc ccgagttgtt gggattccag
     2761 gcatgcatga ccaggctcag ctaatttttg tttttttggt agagacgggg tttcaccata
     2821 ttggccaggc tggtctccaa ctcctaatct caggtgatct acccaccttg gcctcccaaa
     2881 ttgctgggat tacaggcgtg aaccactgct cccttccctg tccttctgat tttaaaataa
     2941 ctataccagc aggaggacgt ccagacacag cataggctac ctggccatgc ccaaccggtg
     3001 ggacatttga gttgcttgct tggcactgtc ctctcatgcg ttgggtccac tcagtagatg
     3061 cctgttgaat tgggtacgcg gccagcggcg agcggtatca gctcactcaa aggcggtaat
     3121 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     3181 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     3241 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     3301 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     3361 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     3421 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     3481 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     3541 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     3601 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     3661 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     3721 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     3781 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     3841 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     3901 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     3961 gtaacctgag gctatggcag ggcctgccgc cccgacgttg gctgcgagcc ctgggccttc
     4021 acccgaactt ggggggtggg gtggggaaaa ggaagaaacg cgggcgtatt ggccccaatg
     4081 gggtctcggt ggggtatcga cagagtgcca gccctgggac cgaaccccgc gtttatgaac
     4141 aaacgaccca acaccgtgcg ttttattctg tctttttatt gccgtcatag cgcgggttcc
     4201 ttccggtatt gtctccttcc gtgtttcagt tagcctcccc ctagggtggg cgaagaactc
     4261 cagcatgaga tccccgcgct ggaggatcat ccagccggcg tcccggaaaa cgattccgaa
     4321 gcccaacctt tcatagaagg cggcggtgga atcgaaatct cgtgatggca ggttgggcgt
     4381 cgcttggtcg gtcatttcga accccagagt cccgctcaga agaactcgtc aagaaggcga
     4441 tagaaggcga tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca
     4501 gcccattcgc cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag
     4561 cgatccgcca cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc
     4621 atgatattcg gcaagcaggc atcgccatgg gtcacgacga gatcctcgcc gtcgggcatg
     4681 ctcgccttga gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga
     4741 tcatcctgat cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc
     4801 gcttggtggt cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca
     4861 gccatgatgg atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc
     4921 acttcgccca atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg
     4981 caaggaacgc ccgtcgtggc cagccacgat agccgcgctg cctcgtcttg cagttcattc
     5041 agggcaccgg acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg
     5101 aacacggcgg catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc
     5161 tccacccaag cggccggaga acctgcgtgc aatccatctt gttcaatcat gcgaaacgat
     5221 cctcatcctg tctcttgatc gatctttgca aaagcctagg cctccaaaaa agcctcctca
     5281 ctacttctgg aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat
     5341 tagtcagcca tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg
     5401 agttaggggc gggactatgg ttgctgacta attgagatgc atgctttgca tacttctgcc
     5461 tgctggggag cctggggact ttccacacct ggttgctgac taattgagat gcatgctttg
     5521 catacttctg cctgctgggg agcctgggga ctttccacac cctaactgac acacattcca
     5581 cagctggttc tttccgcctc aggactcttc ctttttcaat attattgaag catttatcag
     5641 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     5701 gttccgcgca catttccccg aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc
     5761 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     5821 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     5881 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     5941 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     6001 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     6061 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     6121 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga atttt
//
