LOCUS       V030266                 5238 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030266
VERSION     V030266
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..1385
                     /label="Id3(NM_008321)"
                     /note="Id3(NM_008321)"
                     /gene="Id3"
     CDS             1404..1433
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             1452..1475
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(1495..1511)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    1522..2144
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(2293..2881)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    3210..3257
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(3489..4283)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(4318..4675)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(4677..4781)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      4808..5236
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gaaggcgctg agcccggtgc gcggctgcta cgaggcggtg tgctgcctgt
     1081 cggaacgtag cctggccatt gcgcgaggcc gcggtaagag cccgtcgacc gaggagcctc
     1141 ttagcctctt ggacgacatg aaccactgct actcgcgcct gcgggaactg gtgccgggag
     1201 tcccgcgagg cactcagctt agccaggtgg aaatcctgca gcgtgtcata gactacatcc
     1261 tcgaccttca ggtggtcctg gcagagccgg cgcctggacc cccggacggt ccgcatctcc
     1321 cgatccagac agctgagctc actccggaac ttgtgatctc caaggacaag aggagctttt
     1381 gccacacgcg tacgcggccg ctcgagcaga aactcatctc agaagaggat ctggcagcaa
     1441 atgatatcct ggattacaag gatgacgacg ataaggttta aacggccggc cgcggtcata
     1501 gctgtttcct gaacagatcc cgggtggcat ccctgtgacc cctccccagt gcctctcctg
     1561 gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc
     1621 attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt ggtatggagc
     1681 aaggggcaag ttgggaagac aacctgtagg gcctgcgggg tctattggga accaagctgg
     1741 agtgcagtgg cacaatcttg gctcactgca atctccgcct cctgggttca agcgattctc
     1801 ctgcctcagc ctcccgagtt gttgggattc caggcatgca tgaccaggct cagctaattt
     1861 ttgttttttt ggtagagacg gggtttcacc atattggcca ggctggtctc caactcctaa
     1921 tctcaggtga tctacccacc ttggcctccc aaattgctgg gattacaggc gtgaaccact
     1981 gctcccttcc ctgtccttct gattttaaaa taactatacc agcaggagga cgtccagaca
     2041 cagcataggc tacctggcca tgcccaaccg gtgggacatt tgagttgctt gcttggcact
     2101 gtcctctcat gcgttgggtc cactcagtag atgcctgttg aattgggtac gcggccagcg
     2161 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     2221 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     2281 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2341 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2401 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2461 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     2521 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2581 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2641 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2701 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     2761 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2821 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     2881 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     2941 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     3001 atgaagtttt aaatcaatct aaagtatata tgagtaacct gaggctatgg cagggcctgc
     3061 cgccccgacg ttggctgcga gccctgggcc ttcacccgaa cttggggggt ggggtgggga
     3121 aaaggaagaa acgcgggcgt attggcccca atggggtctc ggtggggtat cgacagagtg
     3181 ccagccctgg gaccgaaccc cgcgtttatg aacaaacgac ccaacaccgt gcgttttatt
     3241 ctgtcttttt attgccgtca tagcgcgggt tccttccggt attgtctcct tccgtgtttc
     3301 agttagcctc cccctagggt gggcgaagaa ctccagcatg agatccccgc gctggaggat
     3361 catccagccg gcgtcccgga aaacgattcc gaagcccaac ctttcataga aggcggcggt
     3421 ggaatcgaaa tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt cgaaccccag
     3481 agtcccgctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga
     3541 gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca
     3601 atatcacggg tagccaacgc tatgtcctga tagcgatccg ccacacccag ccggccacag
     3661 tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca
     3721 tgggtcacga cgagatcctc gccgtcgggc atgctcgcct tgagcctggc gaacagttcg
     3781 gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc
     3841 atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc
     3901 ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga
     3961 gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt
     4021 cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac
     4081 gatagccgcg ctgcctcgtc ttgcagttca ttcagggcac cggacaggtc ggtcttgaca
     4141 aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt
     4201 gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg
     4261 tgcaatccat cttgttcaat catgcgaaac gatcctcatc ctgtctcttg atcgatcttt
     4321 gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct cagaggccga
     4381 ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc
     4441 ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta tggttgctga
     4501 ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca
     4561 cctggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg
     4621 ggactttcca caccctaact gacacacatt ccacagctgg ttctttccgc ctcaggactc
     4681 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     4741 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     4801 ccacctgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     4861 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     4921 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     4981 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     5041 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     5101 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     5161 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     5221 aaatttaacg cgaatttt
//
