LOCUS pEnCMV-JAG1(human) 9664 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V021492 VERSION V021492 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 50..429 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 430..633 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 735..4388 /label="JAG1(NM_000214)" /note="JAG1(NM_000214)" /gene="JAG1" misc_feature 4440..5028 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 5094..5142 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 5344..5772 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 5786..6115 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 6182..6976 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 7152..7273 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(7322..7338) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7346..7362 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7370..7400) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7415..7436 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7724..8312) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8483..9343) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9344..9448) /gene="bla" /label="AmpR promoter" ORIGIN 1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg 661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg 721 actctagagc caccatgcgt tccccacgga cgcgcggccg gtccgggcgc cccctaagcc 781 tcctgctcgc cctgctctgt gccctgcgag ccaaggtgtg tggggcctcg ggtcagttcg 841 agttggagat cctgtccatg cagaacgtga acggggagct gcagaacggg aactgctgcg 901 gcggcgcccg gaacccggga gaccgcaagt gcacccgcga cgagtgtgac acatacttca 961 aagtgtgcct caaggagtat cagtcccgcg tcacggccgg ggggccctgc agcttcggct 1021 cagggtccac gcctgtcatc gggggcaaca ccttcaacct caaggccagc cgcggcaacg 1081 accgcaaccg catcgtgctg cctttcagtt tcgcctggcc gaggtcctat acgttgcttg 1141 tggaggcgtg ggattccagt aatgacaccg ttcaacctga cagtattatt gaaaaggctt 1201 ctcactcggg catgatcaac cccagccggc agtggcagac gctgaagcag aacacgggcg 1261 ttgcccactt tgagtatcag atccgcgtga cctgtgatga ctactactat ggctttggct 1321 gcaataagtt ctgccgcccc agagatgact tctttggaca ctatgcctgt gaccagaatg 1381 gcaacaaaac ttgcatggaa ggctggatgg gccccgaatg taacagagct atttgccgac 1441 aaggctgcag tcctaagcat gggtcttgca aactcccagg tgactgcagg tgccagtacg 1501 gctggcaagg cctgtactgt gataagtgca tcccacaccc gggatgcgtc cacggcatct 1561 gtaatgagcc ctggcagtgc ctctgtgaga ccaactgggg cggccagctc tgtgacaaag 1621 atctcaatta ctgtgggact catcagccgt gtctcaacgg gggaacttgt agcaacacag 1681 gccctgacaa atatcagtgt tcctgccctg aggggtattc aggacccaac tgtgaaattg 1741 ctgagcacgc ctgcctctct gatccctgtc acaacagagg cagctgtaag gagacctccc 1801 tgggctttga gtgtgagtgt tccccaggct ggaccggccc cacatgctct acaaacattg 1861 atgactgttc tcctaataac tgttcccacg ggggcacctg ccaggacctg gttaacggat 1921 ttaagtgtgt gtgcccccca cagtggactg ggaaaacgtg ccagttagat gcaaatgaat 1981 gtgaggccaa accttgtgta aacgccaaat cctgtaagaa tctcattgcc agctactact 2041 gcgactgtct tcccggctgg atgggtcaga attgtgacat aaatattaat gactgccttg 2101 gccagtgtca gaatgacgcc tcctgtcggg atttggttaa tggttatcgc tgtatctgtc 2161 cacctggcta tgcaggcgat cactgtgaga gagacatcga tgaatgtgcc agcaacccct 2221 gtttgaatgg gggtcactgt cagaatgaaa tcaacagatt ccagtgtctg tgtcccactg 2281 gtttctctgg aaacctctgt cagctggaca tcgattattg tgagcctaat ccctgccaga 2341 acggtgccca gtgctacaac cgtgccagtg actatttctg caagtgcccc gaggactatg 2401 agggcaagaa ctgctcacac ctgaaagacc actgccgcac gaccccctgt gaagtgattg 2461 acagctgcac agtggccatg gcttccaacg acacacctga aggggtgcgg tatatttcct 2521 ccaacgtctg tggtcctcac gggaagtgca agagtcagtc gggaggcaaa ttcacctgtg 2581 actgtaacaa aggcttcacg ggaacatact gccatgaaaa tattaatgac tgtgagagca 2641 acccttgtag aaacggtggc acttgcatcg atggtgtcaa ctcctacaag tgcatctgta 2701 gtgacggctg ggagggggcc tactgtgaaa ccaatattaa tgactgcagc cagaacccct 2761 gccacaatgg gggcacgtgt cgcgacctgg tcaatgactt ctactgtgac tgtaaaaatg 2821 ggtggaaagg aaagacctgc cactcacgtg acagtcagtg tgatgaggcc acgtgcaaca 2881 acggtggcac ctgctatgat gagggggatg cttttaagtg catgtgtcct ggcggctggg 2941 aaggaacaac ctgtaacata gcccgaaaca gtagctgcct gcccaacccc tgccataatg 3001 ggggcacatg tgtggtcaac ggcgagtcct ttacgtgcgt ctgcaaggaa ggctgggagg 3061 ggcccatctg tgctcagaat accaatgact gcagccctca tccctgttac aacagcggca 3121 cctgtgtgga tggagacaac tggtaccggt gcgaatgtgc cccgggtttt gctgggcccg 3181 actgcagaat aaacatcaat gaatgccagt cttcaccttg tgcctttgga gcgacctgtg 3241 tggatgagat caatggctac cggtgtgtct gccctccagg gcacagtggt gccaagtgcc 3301 aggaagtttc agggagacct tgcatcacca tggggagtgt gataccagat ggggccaaat 3361 gggatgatga ctgtaatacc tgccagtgcc tgaatggacg gatcgcctgc tcaaaggtct 3421 ggtgtggccc tcgaccttgc ctgctccaca aagggcacag cgagtgcccc agcgggcaga 3481 gctgcatccc catcctggac gaccagtgct tcgtccaccc ctgcactggt gtgggcgagt 3541 gtcggtcttc cagtctccag ccggtgaaga caaagtgcac ctctgactcc tattaccagg 3601 ataactgtgc gaacatcaca tttaccttta acaaggagat gatgtcacca ggtcttacta 3661 cggagcacat ttgcagtgaa ttgaggaatt tgaatatttt gaagaatgtt tccgctgaat 3721 attcaatcta catcgcttgc gagccttccc cttcagcgaa caatgaaata catgtggcca 3781 tttctgctga agatatacgg gatgatggga acccgatcaa ggaaatcact gacaaaataa 3841 tcgatcttgt tagtaaacgt gatggaaaca gctcgctgat tgctgccgtt gcagaagtaa 3901 gagttcagag gcggcctctg aagaacagaa cagatttcct tgttcccttg ctgagctctg 3961 tcttaactgt ggcttggatc tgttgcttgg tgacggcctt ctactggtgc ctgcggaagc 4021 ggcggaagcc gggcagccac acacactcag cctctgagga caacaccacc aacaacgtgc 4081 gggagcagct gaaccagatc aaaaacccca ttgagaaaca tggggccaac acggtcccca 4141 tcaaggatta tgagaacaag aactccaaaa tgtctaaaat aaggacacac aattctgaag 4201 tagaagagga cgacatggac aaacaccagc agaaagcccg gtttgccaag cagccggcgt 4261 acacgctggt agacagagaa gagaagcccc ccaacggcac gccgacaaaa cacccaaact 4321 ggacaaacaa acaggacaac agagacttgg aaagtgccca gagcttaaac cgaatggagt 4381 acatcgtata gcttaagggt tcgatcccta ccggttagta atgagtttga tatctcgaca 4441 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc 4501 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta 4561 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt 4621 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg 4681 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta 4741 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt 4801 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg 4861 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca 4921 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc 4981 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaaacgggg 5041 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat 5101 aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt tcataaacgc ggggttcggt 5161 cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg 5221 tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca 5281 acgtcggggc ggcaggccct gccatagcag atctgcgcag ctggggctct agggggtatc 5341 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga 5401 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg 5461 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg catcccttta gggttccgat 5521 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg 5581 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 5641 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt 5701 tataagggat tttggggatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 5761 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc 5821 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa 5881 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 5941 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc 6001 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc 6061 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct 6121 cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg 6181 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt 6241 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc 6301 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact 6361 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt 6421 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca 6481 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat 6541 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg 6601 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga 6661 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga 6721 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa 6781 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga 6841 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt 6901 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct 6961 tgacgagttc ttctgagcgg gactctgggg ttcgcgaaat gaccgaccaa gcgacgccca 7021 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa 7081 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct 7141 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 7201 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 7261 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat 7321 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 7381 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 7441 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 7501 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 7561 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 7621 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 7681 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 7741 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 7801 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 7861 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat 7921 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 7981 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 8041 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 8101 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 8161 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 8221 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 8281 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 8341 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 8401 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 8461 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 8521 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 8581 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 8641 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 8701 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 8761 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 8821 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 8881 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 8941 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 9001 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 9061 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 9121 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 9181 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 9241 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 9301 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 9361 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 9421 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg 9481 acggatcggg agatctcccg atcccctatg gtcgactctc agtacaatct gctctgatgc 9541 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc 9601 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct 9661 tagg //