LOCUS       pEnCMV-JAG1(human)      9664 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V021492
VERSION     V021492
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             735..4388
                     /label="JAG1(NM_000214)"
                     /note="JAG1(NM_000214)"
                     /gene="JAG1"
     misc_feature    4440..5028
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    5094..5142
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5344..5772
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5786..6115
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6182..6976
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    7152..7273
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(7322..7338)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7346..7362
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7370..7400)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7415..7436
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7724..8312)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8483..9343)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9344..9448)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
      721 actctagagc caccatgcgt tccccacgga cgcgcggccg gtccgggcgc cccctaagcc
      781 tcctgctcgc cctgctctgt gccctgcgag ccaaggtgtg tggggcctcg ggtcagttcg
      841 agttggagat cctgtccatg cagaacgtga acggggagct gcagaacggg aactgctgcg
      901 gcggcgcccg gaacccggga gaccgcaagt gcacccgcga cgagtgtgac acatacttca
      961 aagtgtgcct caaggagtat cagtcccgcg tcacggccgg ggggccctgc agcttcggct
     1021 cagggtccac gcctgtcatc gggggcaaca ccttcaacct caaggccagc cgcggcaacg
     1081 accgcaaccg catcgtgctg cctttcagtt tcgcctggcc gaggtcctat acgttgcttg
     1141 tggaggcgtg ggattccagt aatgacaccg ttcaacctga cagtattatt gaaaaggctt
     1201 ctcactcggg catgatcaac cccagccggc agtggcagac gctgaagcag aacacgggcg
     1261 ttgcccactt tgagtatcag atccgcgtga cctgtgatga ctactactat ggctttggct
     1321 gcaataagtt ctgccgcccc agagatgact tctttggaca ctatgcctgt gaccagaatg
     1381 gcaacaaaac ttgcatggaa ggctggatgg gccccgaatg taacagagct atttgccgac
     1441 aaggctgcag tcctaagcat gggtcttgca aactcccagg tgactgcagg tgccagtacg
     1501 gctggcaagg cctgtactgt gataagtgca tcccacaccc gggatgcgtc cacggcatct
     1561 gtaatgagcc ctggcagtgc ctctgtgaga ccaactgggg cggccagctc tgtgacaaag
     1621 atctcaatta ctgtgggact catcagccgt gtctcaacgg gggaacttgt agcaacacag
     1681 gccctgacaa atatcagtgt tcctgccctg aggggtattc aggacccaac tgtgaaattg
     1741 ctgagcacgc ctgcctctct gatccctgtc acaacagagg cagctgtaag gagacctccc
     1801 tgggctttga gtgtgagtgt tccccaggct ggaccggccc cacatgctct acaaacattg
     1861 atgactgttc tcctaataac tgttcccacg ggggcacctg ccaggacctg gttaacggat
     1921 ttaagtgtgt gtgcccccca cagtggactg ggaaaacgtg ccagttagat gcaaatgaat
     1981 gtgaggccaa accttgtgta aacgccaaat cctgtaagaa tctcattgcc agctactact
     2041 gcgactgtct tcccggctgg atgggtcaga attgtgacat aaatattaat gactgccttg
     2101 gccagtgtca gaatgacgcc tcctgtcggg atttggttaa tggttatcgc tgtatctgtc
     2161 cacctggcta tgcaggcgat cactgtgaga gagacatcga tgaatgtgcc agcaacccct
     2221 gtttgaatgg gggtcactgt cagaatgaaa tcaacagatt ccagtgtctg tgtcccactg
     2281 gtttctctgg aaacctctgt cagctggaca tcgattattg tgagcctaat ccctgccaga
     2341 acggtgccca gtgctacaac cgtgccagtg actatttctg caagtgcccc gaggactatg
     2401 agggcaagaa ctgctcacac ctgaaagacc actgccgcac gaccccctgt gaagtgattg
     2461 acagctgcac agtggccatg gcttccaacg acacacctga aggggtgcgg tatatttcct
     2521 ccaacgtctg tggtcctcac gggaagtgca agagtcagtc gggaggcaaa ttcacctgtg
     2581 actgtaacaa aggcttcacg ggaacatact gccatgaaaa tattaatgac tgtgagagca
     2641 acccttgtag aaacggtggc acttgcatcg atggtgtcaa ctcctacaag tgcatctgta
     2701 gtgacggctg ggagggggcc tactgtgaaa ccaatattaa tgactgcagc cagaacccct
     2761 gccacaatgg gggcacgtgt cgcgacctgg tcaatgactt ctactgtgac tgtaaaaatg
     2821 ggtggaaagg aaagacctgc cactcacgtg acagtcagtg tgatgaggcc acgtgcaaca
     2881 acggtggcac ctgctatgat gagggggatg cttttaagtg catgtgtcct ggcggctggg
     2941 aaggaacaac ctgtaacata gcccgaaaca gtagctgcct gcccaacccc tgccataatg
     3001 ggggcacatg tgtggtcaac ggcgagtcct ttacgtgcgt ctgcaaggaa ggctgggagg
     3061 ggcccatctg tgctcagaat accaatgact gcagccctca tccctgttac aacagcggca
     3121 cctgtgtgga tggagacaac tggtaccggt gcgaatgtgc cccgggtttt gctgggcccg
     3181 actgcagaat aaacatcaat gaatgccagt cttcaccttg tgcctttgga gcgacctgtg
     3241 tggatgagat caatggctac cggtgtgtct gccctccagg gcacagtggt gccaagtgcc
     3301 aggaagtttc agggagacct tgcatcacca tggggagtgt gataccagat ggggccaaat
     3361 gggatgatga ctgtaatacc tgccagtgcc tgaatggacg gatcgcctgc tcaaaggtct
     3421 ggtgtggccc tcgaccttgc ctgctccaca aagggcacag cgagtgcccc agcgggcaga
     3481 gctgcatccc catcctggac gaccagtgct tcgtccaccc ctgcactggt gtgggcgagt
     3541 gtcggtcttc cagtctccag ccggtgaaga caaagtgcac ctctgactcc tattaccagg
     3601 ataactgtgc gaacatcaca tttaccttta acaaggagat gatgtcacca ggtcttacta
     3661 cggagcacat ttgcagtgaa ttgaggaatt tgaatatttt gaagaatgtt tccgctgaat
     3721 attcaatcta catcgcttgc gagccttccc cttcagcgaa caatgaaata catgtggcca
     3781 tttctgctga agatatacgg gatgatggga acccgatcaa ggaaatcact gacaaaataa
     3841 tcgatcttgt tagtaaacgt gatggaaaca gctcgctgat tgctgccgtt gcagaagtaa
     3901 gagttcagag gcggcctctg aagaacagaa cagatttcct tgttcccttg ctgagctctg
     3961 tcttaactgt ggcttggatc tgttgcttgg tgacggcctt ctactggtgc ctgcggaagc
     4021 ggcggaagcc gggcagccac acacactcag cctctgagga caacaccacc aacaacgtgc
     4081 gggagcagct gaaccagatc aaaaacccca ttgagaaaca tggggccaac acggtcccca
     4141 tcaaggatta tgagaacaag aactccaaaa tgtctaaaat aaggacacac aattctgaag
     4201 tagaagagga cgacatggac aaacaccagc agaaagcccg gtttgccaag cagccggcgt
     4261 acacgctggt agacagagaa gagaagcccc ccaacggcac gccgacaaaa cacccaaact
     4321 ggacaaacaa acaggacaac agagacttgg aaagtgccca gagcttaaac cgaatggagt
     4381 acatcgtata gcttaagggt tcgatcccta ccggttagta atgagtttga tatctcgaca
     4441 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     4501 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     4561 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     4621 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     4681 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     4741 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     4801 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     4861 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     4921 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     4981 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaaacgggg
     5041 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat
     5101 aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt tcataaacgc ggggttcggt
     5161 cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg
     5221 tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca
     5281 acgtcggggc ggcaggccct gccatagcag atctgcgcag ctggggctct agggggtatc
     5341 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     5401 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     5461 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg catcccttta gggttccgat
     5521 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     5581 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     5641 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     5701 tataagggat tttggggatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     5761 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     5821 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     5881 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     5941 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     6001 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     6061 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     6121 cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg
     6181 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     6241 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     6301 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     6361 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     6421 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     6481 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     6541 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     6601 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     6661 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     6721 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     6781 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     6841 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     6901 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     6961 tgacgagttc ttctgagcgg gactctgggg ttcgcgaaat gaccgaccaa gcgacgccca
     7021 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     7081 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     7141 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     7201 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     7261 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     7321 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     7381 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     7441 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     7501 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     7561 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     7621 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     7681 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     7741 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     7801 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     7861 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat
     7921 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     7981 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     8041 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     8101 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     8161 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     8221 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     8281 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     8341 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     8401 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     8461 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     8521 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     8581 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     8641 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     8701 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     8761 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     8821 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     8881 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     8941 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     9001 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     9061 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     9121 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     9181 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     9241 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     9301 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     9361 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     9421 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
     9481 acggatcggg agatctcccg atcccctatg gtcgactctc agtacaatct gctctgatgc
     9541 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
     9601 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
     9661 tagg
//
