LOCUS       V031242                 7350 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031242
VERSION     V031242
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..3497
                     /label="Jade3(NM_001406241)"
                     /note="Jade3(NM_001406241)"
                     /gene="Jade3"
     CDS             3516..3545
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             3564..3587
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(3607..3623)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    3634..4256
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(4405..4993)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    5322..5369
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(5601..6395)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(6430..6787)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(6789..6893)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      6920..7348
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gaaacggcat agacctgtca gcagcagtga gagttcagat gagtgtcctt
     1081 ccacttcctt tacttctagc tcaatgtata ggaagaagtc aaaaaatcct aaggaacaga
     1141 agaagtctgc tgaggtattc cgaaaggacc tcatcagtgc catgaaaatt cctgactctc
     1201 accatgttaa tcctgacagc tattacctct ttactgacac atggaaagaa gaatgggaaa
     1261 aaggagtcca agtaccagcc aatccagact ctgttccaac gccgtctctc aggattatat
     1321 ctgaaaaagt aaaggagatg ctttttgtcc ggccccggaa atatatccgt tgctccagcc
     1381 cagagtctgc agaacctggc tatatcaata ccctggagca agcagcatct acatgccgct
     1441 atgacctaga tgacatggat atcttttggc ttcaggaact caatgaagac cttggagaaa
     1501 tgggttatgg cccaattgat gagactctta tggaaaagac aatagaagtt ctggaacgcc
     1561 actgccatga aaatatgaac catgctattg aaacagtgga agggctagga atagaatatg
     1621 atgaagatgt gatctgtgat gtgtgccggt ctccagacag tgaagaaggg aatgatatgg
     1681 tattctgtga taagtgtaac gtctgtgtac atcaggcttg ctatggcatt ctcaagattc
     1741 cagaaggtag ttggctgtgc cgctcctgtg tcctgggcat ttatccacag tgtgttttat
     1801 gtcctaagaa aggtggagcc atgaagacca ccagaacagg gactaaatgg gctcatgtca
     1861 gctgtgccct gtggattcca gaagtcagta tcgcttgtcc tgagagaatg gagcctgtca
     1921 caaaaatctc ccatatccca cctagccggt gggccttagt ttgcaacctg tgcaagttga
     1981 agacaggggc ttgtattcag tgctctgtga aaagctgtat cactgccttt catgtcacct
     2041 gtgcctttga gcacggcctg gaaatgaaga ccatcctaga tgagggggat gaagtaaagt
     2101 tcaagtcatt ttgcctcaag catagccaaa acaagccaaa acttggggac gccgagtacc
     2161 accaccacag agtcgccgag cagagccagg caaaaagtga gaaaaccagc ctacgggcac
     2221 agaagcttcg tgaactagaa gaagaattct ataccttagt ccaggtagaa gatgttgcca
     2281 aagagatgga gttatctgcg ttcactgtcg attttatcta taactactgg aaactaaagc
     2341 gcaaaagcaa cttcaataag ccattaattc ccccaaaaga agaggaagaa aatggcttgg
     2401 ttcagccaaa agaagaaagc attcacactc ggatgagaat gtttatgcat ctgcggcagg
     2461 accttgagag ggtccgaaat ctgtgctaca tgataagcag acgagagaag ctgaaactat
     2521 cacacaccaa agtgcaggaa cagatttttg gtttgcaagt ccagcttatc aatgaagaaa
     2581 ttacagaagg actttctttg acaaatgcac tagagaactc actgttttat ccaccaccac
     2641 gaattacctt aaagttaaaa atgcctaaat caacctcaga agattgcaaa gacagctcca
     2701 cagaaactga acaccaactc tcgtctcctg gcagcagctc ccctggtcac agtaaaagga
     2761 gccctcagat gcctgaggaa ccactggata tgaatgtgaa aatatatcca agatatccac
     2821 tagaaagcaa aagtaactgt ttgcagacta gccgaagcca ttctcgatgt gaaaccaaga
     2881 gctccagtcc tactccgaga gctccatctg cagaattcta tcatgggcaa tcactaggga
     2941 agcctctggc ccttcaggct gccctccatg gacaggtgtc tattggtaat gggaagaatc
     3001 agcctaactc cagggtttct agttccaatg gcctggaggg caactggtct gggaacatca
     3061 cccagaaagt taattcaagt gaggtgtgct atgaccagga gtccatgctc agctcccact
     3121 tacccagtcc aggcaacatt agaaaatcta gcatggaaca tttcagcagg tccttcaagg
     3181 aggccaccaa tacatgggtg aagcctactg aggacctcca gtactgtgtt aagccaacta
     3241 agaatgtgag ctctaaggag cagttgtggg gtagacagct tctcaggcgt cccacaggaa
     3301 gagcttcata tcaggaaact gatggctatt gccctgattt ggagcccagt gattcagaag
     3361 cagaagggga ggggagtaaa gaaacaccaa gggtaaagag agagagttct gacagagaaa
     3421 atccttccca tgattctgct cgggagtgcc atgggaaaac caagacacac cctcattccc
     3481 acagctcaat gcaaaggacg cgtacgcggc cgctcgagca gaaactcatc tcagaagagg
     3541 atctggcagc aaatgatatc ctggattaca aggatgacga cgataaggtt taaacggccg
     3601 gccgcggtca tagctgtttc ctgaacagat cccgggtggc atccctgtga cccctcccca
     3661 gtgcctctcc tggccctgga agttgccact ccagtgccca ccagccttgt cctaataaaa
     3721 ttaagttgca tcattttgtc tgactaggtg tccttctata atattatggg gtggaggggg
     3781 gtggtatgga gcaaggggca agttgggaag acaacctgta gggcctgcgg ggtctattgg
     3841 gaaccaagct ggagtgcagt ggcacaatct tggctcactg caatctccgc ctcctgggtt
     3901 caagcgattc tcctgcctca gcctcccgag ttgttgggat tccaggcatg catgaccagg
     3961 ctcagctaat ttttgttttt ttggtagaga cggggtttca ccatattggc caggctggtc
     4021 tccaactcct aatctcaggt gatctaccca ccttggcctc ccaaattgct gggattacag
     4081 gcgtgaacca ctgctccctt ccctgtcctt ctgattttaa aataactata ccagcaggag
     4141 gacgtccaga cacagcatag gctacctggc catgcccaac cggtgggaca tttgagttgc
     4201 ttgcttggca ctgtcctctc atgcgttggg tccactcagt agatgcctgt tgaattgggt
     4261 acgcggccag cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     4321 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     4381 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     4441 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     4501 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     4561 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     4621 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     4681 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     4741 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     4801 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     4861 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     4921 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     4981 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     5041 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     5101 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaac ctgaggctat
     5161 ggcagggcct gccgccccga cgttggctgc gagccctggg ccttcacccg aacttggggg
     5221 gtggggtggg gaaaaggaag aaacgcgggc gtattggccc caatggggtc tcggtggggt
     5281 atcgacagag tgccagccct gggaccgaac cccgcgttta tgaacaaacg acccaacacc
     5341 gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg gttccttccg gtattgtctc
     5401 cttccgtgtt tcagttagcc tccccctagg gtgggcgaag aactccagca tgagatcccc
     5461 gcgctggagg atcatccagc cggcgtcccg gaaaacgatt ccgaagccca acctttcata
     5521 gaaggcggcg gtggaatcga aatctcgtga tggcaggttg ggcgtcgctt ggtcggtcat
     5581 ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc
     5641 tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca
     5701 agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcgatc cgccacaccc
     5761 agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag
     5821 caggcatcgc catgggtcac gacgagatcc tcgccgtcgg gcatgctcgc cttgagcctg
     5881 gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca
     5941 agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat
     6001 gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact
     6061 ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc
     6121 agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc
     6181 gtggccagcc acgatagccg cgctgcctcg tcttgcagtt cattcagggc accggacagg
     6241 tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca
     6301 gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc
     6361 ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa acgatcctca tcctgtctct
     6421 tgatcgatct ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact tctggaatag
     6481 ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg
     6541 cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggac
     6601 tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg
     6661 ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc
     6721 tggggagcct ggggactttc cacaccctaa ctgacacaca ttccacagct ggttctttcc
     6781 gcctcaggac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     6841 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     6901 ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     6961 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     7021 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     7081 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     7141 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     7201 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     7261 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     7321 ctgatttaac aaaaatttaa cgcgaatttt
//
