LOCUS       V026612                 9214 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026612
VERSION     V026612
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..3873
                     /label="KIF11(NM_004523)"
                     /note="KIF11(NM_004523)"
                     /gene="KIF11"
     CDS             3880..3906
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3910..3936
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3940..3966
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     misc_feature    3990..4578
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    4644..4692
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      4894..5322
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5336..5665
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5732..6526
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6702..6823
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6872..6888)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6896..6912
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6920..6950)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6965..6986
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7274..7862)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8033..8893)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8894..8998)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatggc gtcgcagcca
      721 aattcgtctg cgaagaagaa agaggagaag gggaagaaca tccaggtggt ggtgagatgc
      781 agaccattta atttggcaga gcggaaagct agcgcccatt caatagtaga atgtgatcct
      841 gtacgaaaag aagttagtgt acgaactgga ggattggctg acaagagctc aaggaaaaca
      901 tacacttttg atatggtgtt tggagcatct actaaacaga ttgatgttta ccgaagtgtt
      961 gtttgtccaa ttctggatga agttattatg ggctataatt gcactatctt tgcgtatggc
     1021 caaactggca ctggaaaaac ttttacaatg gaaggtgaaa ggtcacctaa tgaagagtat
     1081 acctgggaag aggatccctt ggctggtata attccacgta cccttcatca aatttttgag
     1141 aaacttactg ataatggtac tgaattttca gtcaaagtgt ctctgttgga gatctataat
     1201 gaagagcttt ttgatcttct taatccatca tctgatgttt ctgagagact acagatgttt
     1261 gatgatcccc gtaacaagag aggagtgata attaaaggtt tagaagaaat tacagtacac
     1321 aacaaggatg aagtctatca aattttagaa aagggggcag caaaaaggac aactgcagct
     1381 actctgatga atgcatactc tagtcgttcc cactcagttt tctctgttac aatacatatg
     1441 aaagaaacta cgattgatgg agaagagctt gttaaaatcg gaaagttgaa cttggttgat
     1501 cttgcaggaa gtgaaaacat tggccgttct ggagctgttg ataagagagc tcgggaagct
     1561 ggaaatataa atcaatccct gttgactttg ggaagggtca ttactgccct tgtagaaaga
     1621 acacctcatg ttccttatcg agaatctaaa ctaactagaa tcctccagga ttctcttgga
     1681 gggcgtacaa gaacatctat aattgcaaca atttctcctg catctctcaa tcttgaggaa
     1741 actctgagta cattggaata tgctcataga gcaaagaaca tattgaataa gcctgaagtg
     1801 aatcagaaac tcaccaaaaa agctcttatt aaggagtata cggaggagat agaacgttta
     1861 aaacgagatc ttgctgcagc ccgtgagaaa aatggagtgt atatttctga agaaaatttt
     1921 agagtcatga gtggaaaatt aactgttcaa gaagagcaga ttgtagaatt gattgaaaaa
     1981 attggtgctg ttgaggagga gctgaatagg gttacagagt tgtttatgga taataaaaat
     2041 gaacttgacc agtgtaaatc tgacctgcaa aataaaacac aagaacttga aaccactcaa
     2101 aaacatttgc aagaaactaa attacaactt gttaaagaag aatatatcac atcagctttg
     2161 gaaagtactg aggagaaact tcatgatgct gccagcaagc tgcttaacac agttgaagaa
     2221 actacaaaag atgtatctgg tctccattcc aaactggatc gtaagaaggc agttgaccaa
     2281 cacaatgcag aagctcagga tatttttggc aaaaacctga atagtctgtt taataatatg
     2341 gaagaattaa ttaaggatgg cagctcaaag caaaaggcca tgctagaagt acataagacc
     2401 ttatttggta atctgctgtc ttccagtgtc tctgcattag ataccattac tacagtagca
     2461 cttggatctc tcacatctat tccagaaaat gtgtctactc atgtttctca gatttttaat
     2521 atgatactaa aagaacaatc attagcagca gaaagtaaaa ctgtactaca ggaattgatt
     2581 aatgtactca agactgatct tctaagttca ctggaaatga ttttatcccc aactgtggtg
     2641 tctatactga aaatcaatag tcaactaaag catattttca agacttcatt gacagtggcc
     2701 gataagatag aagatcaaaa aaaggaacta gatggctttc tcagtatact gtgtaacaat
     2761 ctacatgaac tacaagaaaa taccatttgt tccttggttg agtcacaaaa gcaatgtgga
     2821 aacctaactg aagacctgaa gacaataaag cagacccatt cccaggaact ttgcaagtta
     2881 atgaatcttt ggacagagag attctgtgct ttggaggaaa agtgtgaaaa tatacagaaa
     2941 ccacttagta gtgtccagga aaatatacag cagaaatcta aggatatagt caacaaaatg
     3001 acttttcaca gtcaaaaatt ttgtgctgat tctgatggct tctcacagga actcagaaat
     3061 tttaaccaag aaggtacaaa attggttgaa gaatctgtga aacactctga taaactcaat
     3121 ggcaacctgg aaaaaatatc tcaagagact gaacagagat gtgaatctct gaacacaaga
     3181 acagtttatt tttctgaaca gtgggtatct tccttaaatg aaagggaaca ggaacttcac
     3241 aacttattgg aggttgtaag ccaatgttgt gaggcttcaa gttcagacat cactgagaaa
     3301 tcagatggac gtaaggcagc tcatgagaaa cagcataaca tttttcttga tcagatgact
     3361 attgatgaag ataaattgat agcacaaaat ctagaactta atgaaaccat aaaaattggt
     3421 ttgactaagc ttaattgctt tctggaacag gatctgaaac tggatatccc aacaggtacg
     3481 acaccacaga ggaaaagtta tttataccca tcaacactgg taagaactga accacgtgaa
     3541 catctccttg atcagctgaa aaggaaacag cctgagctgt taatgatgct aaactgttca
     3601 gaaaacaaca aagaagagac aattccggat gtggatgtag aagaggcagt tctggggcag
     3661 tatactgaag aacctctaag tcaagagcca tctgtagatg ctggtgtgga ttgttcatca
     3721 attggcgggg ttccattttt ccagcataaa aaatcacatg gaaaagacaa agaaaacaga
     3781 ggcattaaca cactggagag gtctaaagtg gaagaaacta cagagcactt ggttacaaag
     3841 agcagattac ctctgcgagc ccagatcaac cttctcgagt acccatacga cgtacctgat
     3901 tatgcaactt atccttatga tgtacctgat tatgctagct atccttatga tgtcccagac
     3961 tacgcatagt gagggcccga tatctcgaca atcaacctct ggattacaaa atttgtgaaa
     4021 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     4081 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     4141 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     4201 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     4261 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     4321 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     4381 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     4441 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     4501 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     4561 tttgggccgc ctccccgcct ggaaacgggg gaggctaact gaaacacgga aggagacaat
     4621 accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acgggtgttg
     4681 ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac
     4741 cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa
     4801 gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcag
     4861 atctgcgcag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     4921 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     4981 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     5041 taaatcgggg catcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     5101 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     5161 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     5221 tcaaccctat ctcggtctat tcttttgatt tataagggat tttggggatt tcggcctatt
     5281 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     5341 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     5401 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     5461 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     5521 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     5581 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     5641 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     5701 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     5761 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     5821 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     5881 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
     5941 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     6001 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     6061 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     6121 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     6181 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     6241 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     6301 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     6361 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     6421 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     6481 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     6541 ttcgcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     6601 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     6661 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     6721 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     6781 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     6841 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     6901 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     6961 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     7021 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     7081 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     7141 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     7201 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     7261 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7321 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7381 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     7441 tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt
     7501 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     7561 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     7621 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     7681 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     7741 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     7801 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     7861 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     7921 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     7981 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     8041 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     8101 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     8161 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     8221 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     8281 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     8341 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     8401 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     8461 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     8521 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     8581 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     8641 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     8701 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     8761 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     8821 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     8881 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     8941 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     9001 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
     9061 gtcgactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
     9121 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
     9181 cttgaccgac aattgcatga agaatctgct tagg
//
