LOCUS       V018546                 6947 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V018546
VERSION     V018546
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1004..3109
                     /label="KIF3A(NM_001300792)"
                     /note="KIF3A(NM_001300792)"
                     /gene="KIF3A"
     CDS             3113..3142
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             3161..3184
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(3204..3220)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    3231..3853
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(4002..4590)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4919..4966
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(5198..5992)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(6027..6384)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(6386..6490)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      6517..6945
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccgcc accatgccga tcaataaatc
     1021 agagaagcca gaaagctgcg ataatgtgaa ggttgttgtt aggtgccggc ccctcaatga
     1081 gagagagaaa tcaatgtgct acaaacaggc tgtcagtgtg gatgagatga ggggaactat
     1141 cactgtacat aagactgatt cttccaatga acctccaaag acatttactt ttgatactgt
     1201 ttttggacca gagagtaaac aacttgatgt ttataactta actgcaagac ctattattga
     1261 ttctgtactt gaaggctaca atgggactat ttttgcatat ggacaaaccg gaacaggcaa
     1321 aacttttacc atggaaggtg ttcgagctat tcctgaactt agaggaataa ttcccaattc
     1381 atttgctcac atatttggtc atattgcaaa agcggagggt gatacaagat ttttggttcg
     1441 agtgtcttat ttggaaatat ataatgaaga agttcgtgac cttttgggca aggatcagac
     1501 acaaaggtta gaggttaaag aaagacctga tgtgggagtt tatatcaaag atttatcagc
     1561 ttatgtggta aataatgctg atgatatgga tagaattatg acgctaggcc acaaaaatcg
     1621 ttctgttggt gcaactaata tgaacgaaca tagttcccgt tcccatgcca tctttacaat
     1681 tactatagaa tgcagtgaaa aaggcattga tggtaacatg catgtcagga tggggaagct
     1741 ccatcttgta gatcttgctg gttcagaaag acaggcaaaa actggagcta ctggacagcg
     1801 cctaaaggaa gctacaaaaa tcaatctttc actttccacc cttggtaatg taatttctgc
     1861 cttggttgat ggaaaaagca ctcatgtgcc ttatcgtaac tctaaactga ctcgtcttct
     1921 tcaggattcc ttaggaggaa attcaaaaac catgatgtgt gcaaatattg ggccagcaga
     1981 ttacaattat gatgaaacta tcagtacatt acggtatgcc aatcgtgcta agaatattaa
     2041 aaataaagct agaattaatg aagatccaaa ggatgctttg ctgcgtcagt tccagaaaga
     2101 aatagaagaa ctgaaaaaga agcttgaaga aggagaagaa atatcaggct ctgatatcag
     2161 tgggtcagag gaagatgatg atgaagaggg tgaggttgga gaagatggag agaaaaggaa
     2221 aaaaagaagg gatcaagcag gaaaaaagaa agtctcccca gacaagatga ttgaaatgca
     2281 agcaaaaatt gatgaggaga gaaaagcact tgaaacaaag ctcgacatgg aagaagaaga
     2341 aagaaacaag gctagagctg aattagagaa acgggaaaaa gatcttctta aagcccaaca
     2401 agagcatcag tctttgctgg aaaaattatc tgccctggaa aagaaggtaa ttgttggtgg
     2461 ggttgacttg ttggccaaag ctgaggaaca agagaaactt cttgaagaat ctaacatgga
     2521 actggaagaa aggaggaaaa gagcagagca acttcgcaga gaacttgagg aaaaagagca
     2581 agaacgcttg gatattgaag aaaaatatac cagtttgcaa gaggaagcac agggaaagac
     2641 caagaagtta aagaaagttt ggactatgct gatggctgca aagtcagaga tggctgatct
     2701 ccaacaagaa catcagaggg aaattgaagg cctactggag aacattcggc aacttagccg
     2761 ggagcttcga cttcagatgc ttattattga taactttata cctcgggatt atcaggaaat
     2821 gattgaaaac tatgtccatt ggaatgaaga cataggagaa tggcagctaa aatgtgttgc
     2881 ttatacagga aataacatga ggaagcaaac cccagtacct gataaaaagg agaaagatcc
     2941 ctttgaggtg gacctttctc acgtgtatct tgcctatact gaggagagtc tgcgtcagtc
     3001 tttgatgaaa ctagaaagac cacgaacttc aaaggggaaa gcaaggccaa agacagggag
     3061 aagaaagcgt tctgcaaagc ctgaaactgt aattgactct ttactgcagc tcgagcagaa
     3121 actcatctca gaagaggatc tggcagcaaa tgatatcctg gattacaagg atgacgacga
     3181 taaggtttaa acggccggcc gcggtcatag ctgtttcctg aacagatccc gggtggcatc
     3241 cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca gtgcccacca
     3301 gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc ttctataata
     3361 ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca acctgtaggg
     3421 cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa
     3481 tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg ttgggattcc
     3541 aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg ggtttcacca
     3601 tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct tggcctccca
     3661 aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg attttaaaat
     3721 aactatacca gcaggaggac gtccagacac agcataggct acctggccat gcccaaccgg
     3781 tgggacattt gagttgcttg cttggcactg tcctctcatg cgttgggtcc actcagtaga
     3841 tgcctgttga attgggtacg cggccagcgg cgagcggtat cagctcactc aaaggcggta
     3901 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     3961 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     4021 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     4081 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     4141 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     4201 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     4261 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     4321 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     4381 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     4441 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     4501 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     4561 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     4621 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     4681 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     4741 gagtaacctg aggctatggc agggcctgcc gccccgacgt tggctgcgag ccctgggcct
     4801 tcacccgaac ttggggggtg gggtggggaa aaggaagaaa cgcgggcgta ttggccccaa
     4861 tggggtctcg gtggggtatc gacagagtgc cagccctggg accgaacccc gcgtttatga
     4921 acaaacgacc caacaccgtg cgttttattc tgtcttttta ttgccgtcat agcgcgggtt
     4981 ccttccggta ttgtctcctt ccgtgtttca gttagcctcc ccctagggtg ggcgaagaac
     5041 tccagcatga gatccccgcg ctggaggatc atccagccgg cgtcccggaa aacgattccg
     5101 aagcccaacc tttcatagaa ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc
     5161 gtcgcttggt cggtcatttc gaaccccaga gtcccgctca gaagaactcg tcaagaaggc
     5221 gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt
     5281 cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat
     5341 agcgatccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattttcca
     5401 ccatgatatt cggcaagcag gcatcgccat gggtcacgac gagatcctcg ccgtcgggca
     5461 tgctcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca
     5521 gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt
     5581 tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat
     5641 cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg
     5701 gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg
     5761 cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct tgcagttcat
     5821 tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc
     5881 ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc
     5941 tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg
     6001 atcctcatcc tgtctcttga tcgatctttg caaaagccta ggcctccaaa aaagcctcct
     6061 cactacttct ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa
     6121 attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc
     6181 ggagttaggg gcgggactat ggttgctgac taattgagat gcatgctttg catacttctg
     6241 cctgctgggg agcctgggga ctttccacac ctggttgctg actaattgag atgcatgctt
     6301 tgcatacttc tgcctgctgg ggagcctggg gactttccac accctaactg acacacattc
     6361 cacagctggt tctttccgcc tcaggactct tcctttttca atattattga agcatttatc
     6421 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     6481 gggttccgcg cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa
     6541 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     6601 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     6661 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     6721 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     6781 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     6841 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     6901 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaatttt
//
