LOCUS       V030928                 6156 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V030928
VERSION     V030928
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     primer_bind     166..182
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        343..722
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        723..926
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        952..970
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             1029..2303
                     /label="Kcnj6(NM_010606)"
                     /note="Kcnj6(NM_010606)"
                     /gene="Kcnj6"
     CDS             2322..2351
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             2370..2393
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     primer_bind     complement(2413..2429)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     polyA_signal    2440..3062
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     rep_origin      complement(3211..3799)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     polyA_signal    4128..4175
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     CDS             complement(4407..5201)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     promoter        complement(5236..5593)
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     promoter        complement(5595..5699)
                     /gene="bla"
                     /label="AmpR promoter"
     rep_origin      5726..6154
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gacaatggcc aagttaactg aatccatgac taacgtcttg gaaggcgatt
     1081 ccatggacca ggatgtggaa agcccagtgg ccattcacca gccaaagttg cctaagcagg
     1141 ccagggacga cctgccgaga cacatcagcc gagacaggac caaaaggaaa atccagaggt
     1201 acgtgaggaa ggatgggaag tgcaacgttc accacggcaa tgtgcgggag acgtaccgat
     1261 acctgacgga catcttcacc accctggtgg acctgaagtg gagattcaac ctgttgatct
     1321 ttgtcatggt ctacacagtg acgtggcttt tctttgggat gatctggtgg ctgattgcgt
     1381 acatccgggg agatatggac cacatagagg acccctcgtg gactccttgt gtcaccaacc
     1441 tcaacgggtt tgtctctgct tttttattct ccatagagac agaaaccacc atcggttatg
     1501 gctaccgggt catcacggac aagtgccctg aggggattat tctcctctta atccagtccg
     1561 tgttggggtc cattgtcaac gccttcatgg taggatgtat gtttgtgaaa atatcccaac
     1621 ccaagaagag ggcagagacc ctggtctttt ccacccacgc ggtgatctcc atgcgggatg
     1681 ggaaactgtg cttgatgttc cgggtggggg acttgaggaa ttctcacatt gtggaggcat
     1741 ccatcagagc caagttgatc aagtccaaac agacttcaga gggggagttt attcccctca
     1801 accagactga tatcaacgtg gggtactaca caggggacga ccggctcttt ctggtgtcac
     1861 cattgattat tagccatgaa attaaccaac agagtccctt ctgggagatc tccaaagcgc
     1921 agctgcctaa agaggaactg gagattgtgg tcatcctgga gggaatggtg gaagccacag
     1981 gaatgacgtg ccaagcccga agctcctaca tcaccagtga gatcttgtgg ggttaccggt
     2041 tcacacctgt cctaacgctg gaagacgggt tctacgaagt tgactacaac agcttccatg
     2101 agacctatga gaccagcacc ccgtccctta gtgccaaaga gctagcggag ctggctaacc
     2161 gggcagagct gcctctgagt tggtctgtgt ccagcaaact gaaccaacat gcagaattgg
     2221 agacagaaga ggaagagaag aacccggaag aactgacgga gaggaatggt gacgtggcaa
     2281 acctagagaa tgaatccaaa gtaacgcgta cgcggccgct cgagcagaaa ctcatctcag
     2341 aagaggatct ggcagcaaat gatatcctgg attacaagga tgacgacgat aaggtttaaa
     2401 cggccggccg cggtcatagc tgtttcctga acagatcccg ggtggcatcc ctgtgacccc
     2461 tccccagtgc ctctcctggc cctggaagtt gccactccag tgcccaccag ccttgtccta
     2521 ataaaattaa gttgcatcat tttgtctgac taggtgtcct tctataatat tatggggtgg
     2581 aggggggtgg tatggagcaa ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc
     2641 tattgggaac caagctggag tgcagtggca caatcttggc tcactgcaat ctccgcctcc
     2701 tgggttcaag cgattctcct gcctcagcct cccgagttgt tgggattcca ggcatgcatg
     2761 accaggctca gctaattttt gtttttttgg tagagacggg gtttcaccat attggccagg
     2821 ctggtctcca actcctaatc tcaggtgatc tacccacctt ggcctcccaa attgctggga
     2881 ttacaggcgt gaaccactgc tcccttccct gtccttctga ttttaaaata actataccag
     2941 caggaggacg tccagacaca gcataggcta cctggccatg cccaaccggt gggacatttg
     3001 agttgcttgc ttggcactgt cctctcatgc gttgggtcca ctcagtagat gcctgttgaa
     3061 ttgggtacgc ggccagcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     3121 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     3181 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     3241 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     3301 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     3361 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     3421 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     3481 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     3541 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     3601 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     3661 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     3721 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     3781 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     3841 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     3901 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaacctga
     3961 ggctatggca gggcctgccg ccccgacgtt ggctgcgagc cctgggcctt cacccgaact
     4021 tggggggtgg ggtggggaaa aggaagaaac gcgggcgtat tggccccaat ggggtctcgg
     4081 tggggtatcg acagagtgcc agccctggga ccgaaccccg cgtttatgaa caaacgaccc
     4141 aacaccgtgc gttttattct gtctttttat tgccgtcata gcgcgggttc cttccggtat
     4201 tgtctccttc cgtgtttcag ttagcctccc cctagggtgg gcgaagaact ccagcatgag
     4261 atccccgcgc tggaggatca tccagccggc gtcccggaaa acgattccga agcccaacct
     4321 ttcatagaag gcggcggtgg aatcgaaatc tcgtgatggc aggttgggcg tcgcttggtc
     4381 ggtcatttcg aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggcg
     4441 atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg
     4501 ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata gcgatccgcc
     4561 acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc
     4621 ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat gctcgccttg
     4681 agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga
     4741 tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg
     4801 tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg
     4861 gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc
     4921 aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg
     4981 cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt gcagttcatt cagggcaccg
     5041 gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg
     5101 gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa
     5161 gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga tcctcatcct
     5221 gtctcttgat cgatctttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg
     5281 gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc
     5341 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
     5401 cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
     5461 gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct
     5521 gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc acagctggtt
     5581 ctttccgcct caggactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     5641 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     5701 acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt
     5761 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     5821 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     5881 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     5941 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     6001 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     6061 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     6121 aatgagctga tttaacaaaa atttaacgcg aatttt
//
