LOCUS       V040269                 8083 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V040269
VERSION     V040269
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        50..429
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        430..633
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             706..2742
                     /label="MS4A14(NM_032597)"
                     /note="MS4A14(NM_032597)"
                     /gene="MS4A14"
     CDS             2749..2775
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2779..2805
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2809..2835
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     misc_feature    2859..3447
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    3513..3561
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      3763..4191
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4205..4534
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4601..5395
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5571..5692
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5741..5757)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5765..5781
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5789..5819)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5834..5855
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6143..6731)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6902..7762)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7763..7867)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctggcta gcgtttaaac ttaagcttgg
      661 taccgagctc ggatccacta gtccagtgtg gtggaattcg ccaccatgga gtcaacatcc
      721 caggacagaa gggcaactca cgtcatcact ataaaaccaa acgaaactgt attgactgca
      781 tttccctaca gacctcatag ctctctgctg gattttctga agggagagcc aagagtcttg
      841 ggggctaccc agatcctgct tgctctaatc attgtgggct ttggaactat atttgcactt
      901 aattacatcg gtttctccca aagacttccc cttgttgtcc tcacaggata tccattctgg
      961 ggagcactta tttttattct tacaggatac ctcacagtaa ccgataagaa atcaaaactt
     1021 ctgggtcaag gtgtcacggg catgaatgtt atcagctcct tggttgcgat aactgggatt
     1081 actttcacca ttctcagcta cagacatcaa gacaagtact gccagatgcc atcctttgaa
     1141 gaaatatgtg ttttcagtag aactcttttc attgttctgt ttttcttgcc ttcggatgtt
     1201 actcaaaata gtgaacaacc tgccccagaa gaaaatgatc aattacaatt tgtgcttcaa
     1261 gaagagtttt ccagtgatga ttcaacaaca aatgcacaat ctgttatctt tggaggctat
     1321 gctttcttca agttaacact ctctaggagt cctttagtct cccaaccagg taataaaggt
     1381 agagaatttg tgccagatga acaaaagcaa agtatccttc catctcccaa attttcagag
     1441 gaagaaattg aacctttgcc tcccacacta gagaaaaagc cctcagaaaa tatgtccatt
     1501 cagctagact ctacatttaa acaaatgaaa gatgaagatc tacaatctgc tattgtacaa
     1561 ccttctcaaa tgcaaaccaa gcttctgcag gaccaagctg cgtcactcca agtttttcca
     1621 tcccattctg cactaaaact cgaagatata tcacctgaag acttgccatc ccaagctcta
     1681 ccagtagaag gcctgtcaga acaaaccatg ccatctaagt ctacatcatc ccatgtcaaa
     1741 cagtcttcta atctgacagc taatgacctg ccccctcaag gcatactatc ccaagacaca
     1801 tcatctcaag atatgctgtt tcatgacatg acatcccaag atatgcaatc cctagatatg
     1861 ctatctcaag acacaccatc ccacgccatg ccacctcaag acataccttc ccaagatatg
     1921 ctatcccaag ctctatcagc gcatgccata ttacctgaag cctcaacatc ccatattgtg
     1981 cagttccctg aaatacaaca cctacttcag cagcccccag atcttcaacc agaaaacact
     2041 gaacctcaaa accagcaaat tttacaaatg tcatatcaag atattagatc agaagttatg
     2101 gaagagacca aagaatggaa atctgaggag gaactccata gaagaaaatc ctcaagacgg
     2161 cattccttaa accagcaaac caaagccttg caatacttaa ggagacattc tttagacgtg
     2221 caagccaaag gccagaaatc ctcaaagagg cattccttag atcagcaaag caaaggctgg
     2281 caatctccaa agcagaaatc cttagaccag caaatcaaag actggctatc cccaaagagg
     2341 cactccgtag ataagcaagc tcaacttaat caaactaaag agcaactccc agatcagcaa
     2401 gctgaagatc agcaagccaa aggggaacaa tacccagaag gacaatctaa agatggacaa
     2461 gttaaagacc agcagactga taaggagcaa aactcaaaga agcaaaccca ggatcagcaa
     2521 actgaagacc agccggccca agagaagaaa tccccgaaag gacaattcca aaatgttcaa
     2581 gccgaaggac agcaagctca ggtggagaaa gtgccaaaac tgttatgcca agattcagaa
     2641 tcccaaatac agcaatacca attctggcaa ttccacaaag gcaatctcca ggctggacaa
     2701 cccaggactg tcaatctttt ggccaagaat cccctgactg gactcgagta cccatacgac
     2761 gtacctgatt atgcaactta tccttatgat gtacctgatt atgctagcta tccttatgat
     2821 gtcccagact acgcatagtg agggcccgat atctcgacaa tcaacctctg gattacaaaa
     2881 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     2941 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     3001 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     3061 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     3121 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     3181 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     3241 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
     3301 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     3361 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     3421 ggatctccct ttgggccgcc tccccgcctg gaaacggggg aggctaactg aaacacggaa
     3481 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca
     3541 cgggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg
     3601 ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc
     3661 accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg
     3721 ccatagcaga tctgcgcagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     3781 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     3841 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     3901 gtcaagctct aaatcggggc atccctttag ggttccgatt tagtgcttta cggcacctcg
     3961 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     4021 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     4081 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttggggattt
     4141 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
     4201 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     4261 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     4321 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     4381 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     4441 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     4501 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
     4561 tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
     4621 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     4681 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     4741 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct
     4801 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     4861 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     4921 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     4981 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     5041 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     5101 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac
     5161 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     5221 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     5281 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     5341 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
     5401 actctggggt tcgcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg
     5461 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     5521 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
     5581 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     5641 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     5701 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     5761 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     5821 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     5881 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     5941 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     6001 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     6061 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     6121 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     6181 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     6241 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     6301 ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca
     6361 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     6421 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     6481 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     6541 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     6601 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     6661 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     6721 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     6781 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     6841 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     6901 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     6961 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     7021 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     7081 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     7141 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     7201 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     7261 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     7321 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     7381 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     7441 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     7501 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     7561 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     7621 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     7681 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     7741 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     7801 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     7861 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga
     7921 tcccctatgg tcgactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct
     7981 gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac
     8041 aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agg
//
